Fascaplysin sensitizes cells to TRAIL-induced apoptosis through upregulating DR5 expression |
| |
Authors: | WANG Feng CHEN Haimin YAN Xiaojun ZHENG Yanling |
| |
Institution: | 1. Department of Clinical Laboratory, Lihuili Hospital of Ningbo Medical Center, Ningbo 315041, China 2. Ningbo University, Key Laboratory of Applied Marine Biotechnology, Ministry of Education, Ningbo 315211, China |
| |
Abstract: | This study investigated the molecular mechanism of anti-tumor effect of fascaplysin, a nitrogenous red pigment firstly isolated from a marine sponge. Microarray analysis show that the TNF and TNF receptor superfamily in human umbilical vein endothelial cells (HUVEC) and human hepatocarcinoma cells (BEL-7402) were significantly regulated by fascaplysin. Western Blot results reveal that fascaplysin increased the expression of cleaved caspase-9, active caspase-3, and decreased the level of procaspase-8 and Bid. Flow cytometry and cytotoxicity tests indicate that fascaplysin sensitized cells to tumor necrosis-related apoptosisinducing ligand-(TRAIL) induced apoptosis, which was markedly blocked by TRAIL R2/Fc chimera, a dominant negative form of TRAIL receptor DR5. Therefore, our results demonstrate that fascaplysin promotes apoptosis through the activation of TRAIL signaling pathway by upregulating DR5 expression. |
| |
Keywords: | fascaplysin apoptosis DR5 TRAIL microarray |
本文献已被 CNKI 万方数据 SpringerLink 等数据库收录! |
|