Optimization of conjugated linoleic acid triglycerides via enzymatic esterification in no-solvent system |
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| Authors: | Yi Dan Sun Xiuqin Li Guangyou Liu Fayi Lin Xuezheng Shen Jihong |
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| Institution: | [1]College of Life Sciences. Ocean University of China, Qingdao 266003, China [2]Key Laboratory of Marine Bio-active Substance, First Institute of Oceanography, State Oceanic Administration, Qingdao 266061. China |
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| Abstract: | We compared four esterifiable enzymes.The lipase Novozym 435 possessed the highest activity for the conjugated linoleic acid esterification during the synthesis of triglycerides.The triglycerides were synthesized by esterification of glycerol and conjugated linoleic acid(CLA) in a no-solvent system using lipase catalysis.We investigated the effects of temperature,enzyme concentration,water content,and time on esterification.Enzyme and water concentrations of up to 1% of the total reaction volume and a syste... |
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| Keywords: | conjugated linoleic acid glycerol lipase esterification gyrating sieve |
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