海参花营养成分分析及其提取物对人卵巢癌细胞雌二醇分泌的影响

海参花是雌海参的性腺，在海参加工过程中常被作为下脚料，但它含有丰富的营养成分和多种活性因子，本研究检测了海参花的一般营养物质、元素含量以及氨基酸组成，同时，研究了海参花水提取物和乙醇提取物对人卵巢癌OVCAR 3细胞增殖以及对其雌二醇分泌的影响。结果表明，海参花含水量为86.63%，粗蛋白、粗脂质和粗多糖（干重）占比分别为65.06%、14.82%和1.93%。共检测出17种氨基酸，总氨基酸含量为519.09 mg/kg。其中，必需氨基酸含量为197.04 mg/kg。海参花还含有多种对人体有益的元素。海参花提取物对人卵巢癌OVCAR 3细胞的增殖有明显的抑制作用，海参花水提取物、乙醇提取物均可提高细胞培养液上清液中雌二醇的含量，其中乙醇提取物可显著提高上清液中雌二醇的含量（P<0.01）。以上表明，海参花的营养丰富，其提取物能显著促进人卵巢癌OVCAR 3细胞雌二醇的分泌调节，因此具有很高的开发利用价值。

Nutritional composition analysis of sea cucumber ovum and the effect of extracts on estradiol secretion in ovarian cancer cells

Sea cucumber ovum is the gonad of female sea cucumber ovum, which is often treated as the waste in sea cucumber processing, but it contains rich nutrients and a variety of active factors. In this study, the composition and content of general nutrients, elements and amino acids of sea cucumber ovum were analyzed. At the same time, the effects of sea cucumber ovum water extract and alcohol extract on the proliferation of human ovarian cancer OVCAR-3 cells and its estradiol secretion were studied. Results showed that the water content of sea cucumber ovum was 86.63%, and the contents of crude protein, crude lipid and crude polysaccharide (dry weight) were 65.06%, 14.82% and 1.93%, respectively. A total of 17 amino acids were detected, and the total amino acids content was 519.09 mg/kg. Among them, the content of essential amino acids was 197.04 mg/kg. Sea cucumber ovum contains a variety of elements beneficial to human body. Sea cucumber ovum extract significantly inhibited the proliferation of human ovarian cancer OVCAR-3 cells, and the contents of estradiol in the supernatant of cell culture solution were increased by water extract and alcohol extract in sea cucumber ovum, and the content of estradiol in supernatant can be significantly increased by alcohol extract(P<0.01). The above results show that sea cucumber ovum is rich in nutrition, and its extract has a significant role in promoting the secretion and regulation of estradiol in human ovarian cancer OVCAR-3 cells, so it has high exploitation and utilization values.