贵阳市"两湖一库"不同季节硫酸盐还原菌分布变化

基于MPN法对贵阳市"两湖一库"——阿哈水库、红枫湖、百花湖不同季节沉积物中硫酸盐还原菌(SRB)分布规律及其影响因素进行了研究.结果表明,"两湖一库"SRB含量及分布季节差异显著,冬季SRB含量明显低于夏、秋季节.冬季,SRB含量峰值主要集中在沉积物中部,而在夏、秋季节,SRB含量峰值主要集中在沉积物上部.在受到酸性矿山废水污染的阿哈水库,硫酸根含量远高于红枫湖和百花湖,但沉积物中的SRB含量整体上和其它2个未受硫酸盐影响的湖泊差异不大,仅在夏、秋季节表层沉积物中明显升高,表明"两湖一库"丰富的有机质及适宜的pH为硫酸盐还原菌提供了良好的生长环境,硫酸根含量没有成为SRB含量的主要限制因素.

Variations of sulfate reducing bacteria's distribution in sediments of Aha Reservoir, Lake Hongfeng and Lake Baihua, Guiyang City in different seasons

In the present study, we investigated spatial and temporal distributions of sulfate reducing bacteria (SRB) and controlling factors in sediment of Aha Reservoir, Lake Hongfeng and Lake Baihua, Guiyang City based on the most probable number (MPN) method. The results indicate that there were discernible seasonal variations in the SRB content distribution. SRB contents of sediments in winter were much less than those in summer and fall. The peaks of SRB contents occurred in the middle of sediment cores in winter, while SRB generally increased towards the sediment-water interface in June and September. The contents of sulfate in the sediments in Aha Reservoir which has been polluted by acid mine drainage were much higher than those in the other two reservoirs:Lake Hongfeng and Lake Baihua. SRB contents, however, have no discernible spatial variations in the three lakes(reservoirs), except for elevated values only in the surface of sediment in Lake Aha in summer and autumn. This indicates that higher organic matter contents and optimum pH in these reservoirs favor the growth of SRB, and sulfate content was not the main significant factor of SRB growth.