海水油荧光测量方法实验研究

根据荧光测量方法的原理，采和双光路，双通道及单片微机控制，数据自动采集的技术，于１９９７年研制成功用于海洋现场探测的单波段水中油荧光计。应用该水中油荧光计进行了海水中微量油份的测量实验研究。

EXPERIMENTAL STUDY ON FLUORIMETRY FOR MEASURING OIL IN SEAWATER

The portable oil measuring instrument, using modern fluorimetry, double channel and single-chip micro-computer control technique, is a new marine optical electronic instrument used for measuring trace-oil in seawater. The instrument can detect fluorescence signals produced by oil in seawater, adopting xanon lamp with low consumption, low repeat frequency and pulse detecting techniques. It works well from sea surface to 25m depth under any light condition. In September 1997, experimental measurement of the mixture consisting of filtered Qingdao seawater and oil was performed in a laboratory tank, using the oil in-situ fluorometer (OISF). Various oil concentrations were made by addition of known amounts of oil insolution to a known volume of water, the fluorescence was measured for each concentration and the calibration curves were acquired. The experimental results show that under the same experimental condition, correlation between the OISF data and laboratory fluorometer Rf-540 data is very well with r?0.995. Assuming no fluorescence contaminants (e.g. yellow, inorganic and planktonic substances) its measuring range for oil in the water is from 1×10^-9g/ml to 1×10^-5g/ml. In practical marine measurements, the influence of natural fluorescence substance on measuremental results cannot be eliminated fully, due to the limit of excitation and emission filters of the oil in-situ fluorometer. Therefore, the effect of fluorescence contaminants must be taken into account when in-water oil is measured by the fluorometer. While in-situ measurement is performed, surface water samples should be collected and analyzed by the fluorometer and GC/MC instruments. Finally, the acquired sample concentration and analytic results of the yellow substance, plankton et al. should be used for correcting and validating calibration curves of the fluorometer in laboratory.