| 凡纳滨对虾IHHNV群体遗传多样性的SSR分析 |
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| 引用本文： | 陈秀荔,牛生洋,赵永贞,彭敏,杨春玲,崔亮,蒋伟明,李咏梅,陈晓汉.凡纳滨对虾IHHNV群体遗传多样性的SSR分析[J].广东海洋大学学报,2012(3):29-34. |
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| 作者姓名： | 陈秀荔 牛生洋 赵永贞 彭敏 杨春玲 崔亮 蒋伟明 李咏梅 陈晓汉 |
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| 作者单位： | 1. 广西壮族自治区水产研究所,广西水产遗传育种与健康养殖重点实验室,广西南宁530021
2. 河南科技学院,河南新乡453003 |
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| 基金项目： | 广西科学研究与技术开发计划项目(桂科攻11107012-1-3);广西农业科技成果转化资金项目(桂科转10100017-6);广西区水产畜牧兽医局科研计划项目(桂渔牧科09254-04);广西水产遗传育种与健康养殖重点实验室开放课题(GXKL-AQUA-2011-04) |
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| 摘 要： | 利用SSR标记分析广西凡纳滨对虾育种中心选育的凡纳滨对虾品种中抗传染性皮下及造血组织坏死病毒(IHHNV)家系的遗传多样性,为凡纳滨对虾抗IHHNV育种亲本材料的选择提供参考。通过体外攻毒试验,从育种中心构建的凡纳滨对虾家系中筛选出抗IHHNV的家系,然后利用SSR标记对筛选出的抗IHHNV品种的遗传多样性进行分析。结果表明,6对引物在上述抗病毒品种中共检测到162个等位变异,每对引物平均为3个,引物的多态信息含量(PIC)在0.372 8～0.479 7之间,平均为0.434 1;抗病品种间的遗传相似系数平均为0.894 2,表明筛选出的抗病品种遗传多样性较高。
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| 关 键 词： | 凡纳滨对虾 IHHNV SSR 遗传多样性 |
Analysis of Genetic Diversity of Litopenaeus vannamei Varieties Resistant to IHHNV Based on SSR Markers |
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| CHEN Xiu-li,ZHAO Yong-zhen,PENG Min,YANG Chun-ling,CUI Liang,JIANG Wei-ming,LI-Yong-mei,CHEN Xiao-han.Analysis of Genetic Diversity of Litopenaeus vannamei Varieties Resistant to IHHNV Based on SSR Markers[J].Journal of Zhanjiang Ocean University,2012(3):29-34. |
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| Authors: | CHEN Xiu-li ZHAO Yong-zhen PENG Min YANG Chun-ling CUI Liang JIANG Wei-ming LI-Yong-mei CHEN Xiao-han |
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| Institution: | 1(1 Guangxi Key Laboratory of Aquatic Genetic Breeding and Healthy Aquaculture,Guangxi Institute of Fisheries,Nanning 530021,China;2.Henan Institute of Science and Technology,Xinxiang 453003,China) |
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| Abstract: | Litopenaeus vannamei from Shrimp Breeding Center of Guangxi were infected by injecting infectious hypodermal and hematopoietic necrosis virus(IHHNV) in vitro.PCR and real-time PCR were used to screen of the IHHNV-resistant strains,and the genetic diversity of anti-IHHNV strains was analyzed based on SSR markers.162 alleles were obtained among the anti-IHHNV strains by using six primers,three on average,and polymorphic information content(PIC) was 0.372 8-0.479 7,and the average was 0.434 1.The average genetic similarity coefficient between the resistant varieties was 0.894 2.The result suggested that the genetic diversity of disease-resistant race were higher. |
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| Keywords: | Litopenaeus vannamei IHHNV SSR marker genetic diversity |
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