鳗弧菌感染对斑节对虾免疫相关指标的影响

为了研究鳗弧菌(Vibrio anguillarum)对斑节对虾(Penaeus monodon)免疫相关指标的影响,分别对斑节对虾注射生理盐水和鳗弧菌,测定了不同时间点肝胰腺和鳃中总抗氧化能力(T-AOC)、溶菌酶(LSZ)和诱导型一氧化氮合酶(i NOS)活性以及一氧化氮(NO)含量的变化。结果显示,与对照组相比,感染鳗弧菌后,肝胰腺和鳃中T-AOC活性分别于6和12 h达到最大值(P0.05),随后逐渐降低;肝胰腺和鳃中LSZ活性分别于6和12 h升高至最大值,随后均于24 h显著低于对照组(P0.05);肝胰腺和鳃中NO含量分别于6和3 h达到最大值,随后肝胰腺中NO含量于24 h降低至最小值,而鳃中NO含量于6和24 h虽有降低,但仍显著高于对照组;肝胰腺中i NOS对感染反应较为敏感,活性于3 h达到最大值,而鳃中i NOS活性于6 h开始显著上升,并于12 h达到最大值(P0.05)。研究表明,长时间的鳗弧菌感染对斑节对虾免疫相关指标有显著影响;T-AOC、LSZ、NO和i NOS对鳗弧菌感染均反应敏感,可以作为弧菌病诱发的监测指标。

The non-specific immune related indexes response of Penaeus monodon to Vibrio anguillarum challenge

To investigate the effect of Vibrio anguillarum challenge on the non-specific immune related indexes of Penaeus monodon, healthy P. monodon were injected with sterile 0.86% normal saline and V. anguillarum, respectively. Total antioxidant capacity (T-AOC), lysozyme (LSZ), and nitrio oxide synthase (iNOS) activities and nitrio oxide (NO) content in hepatopancreas and gills of P. monodon were measured at different sampling time point. The results showed that: compared with the control group, after challenged with V. anguillarum, T-AOC activities in hepatopancreas and gills both increased to the highest at 6 and 12 h (P<0.05), respectively, and then decrease gradually. LSZ activities in hepatopancreas and gills increased to the peak value at 6 and 12 h (P<0.05), respectively, and then decreased gradually to a lower level than the control at 24 h (P<0.05). NO content increased significantly to the highest at 6 and 3 h, respectively, then decreased to the lowest at 24 h. Although the NO content in gills showed a downward tendency, it was still significantly higher than the control group (P<0.05). iNOS in hepatopancreas was sensitive to V. anguillarum challenge, and the activity increased to the highest at 3 h, but iNOS activities in gills began to increase significantly at 6 h and reach the highest at 12 h (P<0.05). The results indicated that V. anguillarum challenge had significant impact on the immune enzyme activities of P. monodon. T-AOC, LSZ, NO and iNOS were all sensitive to V. anguillarum challenge, and could be regarded as monitor indexes for Vibrio diseases.