| Efficient expression of green fluorescent protein （GFP） mediated by a chimeric promoter in Chlamydomonas reinhardtii |
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| 引用本文： | 吴锦霞,胡章立,王潮岗,黎双飞,雷安平.Efficient expression of green fluorescent protein （GFP） mediated by a chimeric promoter in Chlamydomonas reinhardtii[J].中国海洋与湖沼学报,2008,26(3). |
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| 作者姓名： | 吴锦霞 胡章立 王潮岗 黎双飞 雷安平 |
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| 作者单位： | Institute of Ecology and Environment，College of Life Sciences，Shenzhen University，Shenzhen，518060，China |
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| 基金项目： | 国家高技术研究发展计划(863计划)，广东省自然科学基金，广东省深圳市科技攻关项目 |
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| 摘 要： | To improve the expression efficiency of exogenous genes in Chlamydomonas reinhardtii,a high efficient expression vector was constructed.Green fluorescent protein(GFP) was expressed in C.reinhardtii under the control of promoters:RBCS2 and HSP70A-RBCS2.Efficiency of transformation and expression were compared between two transgenic algae:RBCS2 mediated strain Tran-Ⅰ and HSP70A-RBCS2 mediated strain Tran-Ⅱ.Results show that HSP70A-RBCS2 could improve greatly the transformation efficiency by approximately eightfold of RBCS2,and the expression efficiency of GFP in Tran-Ⅱ was at least double of that in Tran-Ⅰ.In addition,a threefold increase of GFP in Tran-Ⅱwas induced by heat shock at 40°C.All of the results demonstrated that HSP70A-RBCS2 was more efficient than RBCS2 in expressing exogenous gene in C.reinhardtii.
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| 关 键 词： | 表达效率 绿色荧光性蛋白质 HSP70A-RBCS2 RBCS2 转换效率 |
Efficient expression of green fluorescent protein (GFP) mediated by a chimeric promoter in Chlamydomonas reinhardtii |
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| WU Jinxia,HU Zhangli,WANG Chaogang,LI Shuangfei,LEI Anping Institute of Ecology and Environment,College of Life Sciences,Shenzhen University,Shenzhen,China.Efficient expression of green fluorescent protein (GFP) mediated by a chimeric promoter in Chlamydomonas reinhardtii[J].Chinese Journal of Oceanology and Limnology,2008,26(3). |
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| Authors: | WU Jinxia HU Zhangli WANG Chaogang LI Shuangfei LEI Anping Institute of Ecology and Environment College of Life Sciences Shenzhen University Shenzhen China |
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| Institution: | Institute of Ecology and Environment, College of Life Sciences, Shenzhen University, Shenzhen, 518060, China |
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| Abstract: | To improve the expression efficiency of exogenous genes in Chlamydomonas reinhardtii, a high efficient expression vector was constructed. Green fluorescent protein (GFP) was expressed in C. Reinhardtii under the control of promoters: RBCS2 and HSP70A-RBCS2. Efficiency of transformation and expression were compared between two transgenic algae: RBCS2 mediated strain Tran-I and HSP70A-RBCS2 mediated strain Tran-II. Results show that HSP70A-RBCS2 could improve greatly the transformation efficiency by approximately eightfold of RBCS2, and the expression efficiency of GFP in Tran-II was at least double of that in Tran-I. In addition, a threefold increase of GFP in Tran-II was induced by heat shock at 40°C. All of the results demonstrated that HSP70A-RBCS2 was more efficient than RBCS2 in expressing exogenous gene in C. Reinhardtii. |
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| Keywords: | expression efficiency green fluorescent protein (GFP) HSP70A-RBCS2 RBCS2 transformation efficiency |
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