海洋来源白浅灰链霉菌中环二肽类化合物的分离与结构鉴定

为了寻找新的抗肿瘤活性化合物，采用硅胶、Sephadex LH-20柱色谱和高效液相色谱，从白浅灰链霉菌Streptomyces albogriseolus(A2002)中分离得到3个环二肽；利用理化及波谱学方法鉴定其结构分别为环(丙氨酸-缬氨酸)[cyclo-(Ala-Val)，1]，环(丙氨酸-脯氨酸)[cyclo-(Pro-Ala)，2]和环(脯氨酸-酪氨酸)[cyclo-(Pro-Tyr)，3]。

Isolation and Identification of Cyclic Dipeptides from a Marine-Derived Streptomyces albogriseolus

In the course of our screening for new anti-tumor compounds from marine-derived microbial strains, it was found that the acetonic extract from the fermentation of Streptomyces albogriseolus A2002, a marine-derived actinomycete strain isolated from a Jiaozhou Bay sediment sample, significantly inhibited the cell cycle of tsFT210 cells at the G2/M phase and induced apoptosis in tsFT210 cells. Thus, the chemical examination was undertaken on the constituents of bioactive fractions obtained by bioassay-guided fractionation from the fermentation of producing strains. Compounds 1-3 were isolated by repeated column chromatography over Sephadex LH-20 and silica gel, followed by the prepared HPLC separation. Their structures were identified as cyclic dipeptides, cyclo-(Ala-Val) (1), cyclo-(Ala-Pro) (2) and cyclo-(Pro-Tyr) (3), respectively, by the spectroscopic method.