Isozyme analysis of spotted halibut Verasper variegatus temminck et schlegel |
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| Authors: | Liu Manhong Gao Tianxiang Zhang Xiumei and Chen Siqing |
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| Institution: | (1) The Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, 266003 Qingdao, P.R.China;(2) Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, 266071 Qingdao, P.R.China |
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| Abstract: | To investigate the tissue-specificities of isozymes and the genetic structure of wild spotted halibut (Verasper variegates) population, horizontal starch gel electrophoresis was performed on 45 individuals collected in part of the Yellow Sea. The
performances of 17 isozymes in 8 kinds of tissues or organs were screened preliminarily in a TC-7.0 buffer system. The results
showed that the screened isozymes displayed remarkable tissue-specificities. Finally, 14 enzymes (AAT, ADH, EST, GPI, G3PDH,
IDHP, LAP, LDH, MDH, MPI, PGDH, PGM, SDH and SOD) and 4 kinds of tissues (eye, skeleton muscle, liver and heart) were selected
for genetic analysis. Fourteen isozymes are encoded by 20 loci, and 9 of them are polymorphic. The polymorphic loci are AAT-1*.
GPI-2* G3PDH*, IDHP-1*, LDH*, MPI*, PGM-1*. PGM-2* and SDH*, and the proportion of polymorphic loci is 0.4500 (P0.99). The mean values of observed and expected heterozygosities are 0.027 8 and 0.026 5, respectively and the average effective
number of alleles is 1.067 5. |
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| Keywords: | Verasper variegatus isozyme tissue-specificity genetic structure |
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