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Genetic discrimination for three gynogenetic clones of silver carp Hypophthalmichthys molitrix, based on restriction endonuclease analysis of Nd5-Nd6 region of mitochondrial DNA
作者姓名:周建峰  叶玉珍  吴清江
作者单位:[1]State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China
基金项目:Project supported by the National Natural Science Foundation of China No. 39830300).
摘    要:1 INTRODUCTION Silver carp (Hypophthalmichthys molitrix) is an important species in freshwater fisheries and ranks the first in world fish production (Li, 1993). China has a long history of aquaculture of silver carp that is very difficult for selective breeding because it has a long life cycle. However, development of cytoge-netics and modern molecular genetics built up a strong impact to the research on selective breeding. A rapid, efficient approach for establishment of a pure line of…

关 键 词:鲤鱼  克隆技术  遗传起源  雌性  DNA
收稿时间:2003-07-20
修稿时间:2003-12-29

Genetic discrimination for three gynogenetic clones of silver carp Hypophthalmichthys molitrix, based on restriction endonuclease analysis of Nd5-Nd6 region of mitochondrial DNA
Zhou JianFeng;Xie YuZhe;Wu QingJiang.Genetic discrimination for three gynogenetic clones of silver carp Hypophthalmichthys molitrix, based on restriction endonuclease analysis of Nd5-Nd6 region of mitochondrial DNA[J].Chinese Journal of Oceanology and Limnology,2005,23(1):126-128.
Authors:Zhou JianFeng;Xie YuZhe;Wu QingJiang
Abstract:Three artificial gynogenetic clones of silver carp were produced for the analysis of restriction enzyme digestion patterns of ND5-ND6 region from mtDNA of the clones. It is revealed that all intraclonal individuals shared completely the same digestion patterns but among interclonal individuals did not. The three clones were mixed and cultured in a pond together for two years, and restriction endonuclease digestion patterns of ND5-ND6 were used as genetic markers to assess the growth performance of each clone.
Keywords:mtDNA  molecular markers  gynogenesis  silver carp
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