| DABS-Cl柱前衍生高效液相色谱法测定对虾肌肉中18种游离氨基酸 |
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| 引用本文： | 吴成福,林黑着,李刘冬,江世贵.DABS-Cl柱前衍生高效液相色谱法测定对虾肌肉中18种游离氨基酸[J].广东海洋大学学报,2007,27(6):62-66. |
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| 作者姓名： | 吴成福 林黑着 李刘冬 江世贵 |
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| 作者单位： | 1. 广东海洋大学水产学院,广东,湛江,524088;中国水产科学研究院南海水产研究所,广东,广州,510300
2. 中国水产科学研究院南海水产研究所,广东,广州,510300 |
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| 基金项目： | 国家高技术研究发展计划(863计划);农业结构调整项目 |
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| 摘 要： | 建立一种以DABS-Cl为柱前衍生剂,利用反相高效液相色谱测定对虾肌肉中18种游离氨基酸的方法。结果表明,在50 min内18种氨基酸得到完全分离,各氨基酸相关系数均大于0.997,氨基酸的回收率在86.60%~107.27%之间(胱氨酸除外),方法的检出限为0.18~0.66 mg/L,相对标准偏差为3.53%~8.51%(天冬氨酸除外)。
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| 关 键 词： | 对虾肌肉 游离氨基酸 高效液相色谱法 柱前衍生剂 DABS-Cl |
| 文章编号： | 1673-9159(2007)06-0062-05 |
| 修稿时间： | 2007年5月29日 |
Determination of 18 Free Amino Acids in the Muscle of Shrimp by Reversed-Phase High Performance Liquid Chromatography with DABS-Cl Precolumn Derivatization |
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| WU Cheng-fu,LIN Hei-zhao,LI Liu-dong,JIANG Shi-gui.Determination of 18 Free Amino Acids in the Muscle of Shrimp by Reversed-Phase High Performance Liquid Chromatography with DABS-Cl Precolumn Derivatization[J].Journal of Zhanjiang Ocean University,2007,27(6):62-66. |
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| Authors: | WU Cheng-fu LIN Hei-zhao LI Liu-dong JIANG Shi-gui |
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| Abstract: | By a reversed-phase high performance liquid chromatographic method,simultaneous determination of 18 free amino acids in the muscle of shrimp was carried out.The samples were derivatized with DABS-Cl.The results showed that 18 free amino acids were easily separated and determined at one run within 50 min.The amino acid amounts were linearly related with the peak areas and the correlated coefficient were above 0.997.The recoveries of amino acids added were 86.60%-107.27%,except cystine.The detection limit of amino acids ranged from 0.18 to 0.66 mg/L.There were 3.53%-8.51% relative standard deviations in these amino acids,except aspartie. |
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| Keywords: | DABS-Cl |
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