3种海洋微藻微胶囊培养的初步研究

用12mg/mL羧甲基纤维素钠(CMC)、10mg/mL海藻酸钠(SA)和0.5mmol/mL CaCl2制备亚心形扁藻、绿色杜氏藻和球等鞭金藻8701的中空海藻酸钙微胶囊,3种微藻在微胶囊内生长表现突出,最高细胞密度亚心形扁藻为70.4×106/mL,绿色杜氏藻为100.9×106/mL,球等鞭金藻8701为264.1×106/mL,分别是悬浮培养微藻最高密度的35倍、27倍和84倍,结果说明微胶囊化培养是微藻高密度培养的有效方式。     

硝酸益康唑微囊化技术研究

以明胶与阿拉伯胶配比为囊材,Span60为乳化剂,戊二醛为交联剂,采用复凝聚法制备了硝酸益康唑微胶囊,并研究了制备工艺条件对微胶囊形态、粒度、药物包封率及释放特性的影响。结果表明,随药物与囊材配比增大,形成的微胶囊数目越多、粒径越小,包封率降低,释放速度增大;戊二醛的用量对微胶囊膜的机械强度、微胶囊形状和粒度及分散性有显著影响。     

Drying of Micro-Encapsulated Lactic Acid Bacteria- Effects of Trehalose and Immobilization on Cell Survival and Release Properties

Lactic acid bacteria （LAB） were encapsulated with alginate, gelatin and trehalose additives by the extrusion method and dried at 4 ℃. The microcapsules were generally spherical and had a wrinkled surface with a size of 1.7mm± 0.2mm Trehalose as a carbohydrate source in the culture medium could reduce acid production and performed no function in the positive proliferation of LAB. Using trehalose as a carbohydrate source and protective medittm simultaneously had a benefit in the protection of LAB cells during the storage at 4 ℃. The density of live LAB cells could be 10^7 CFU g^-1 after 8weeks of storage. Cells of LAB could be continuously released from the capsules from the acidic （pH 1.2） to neutral conditions （pH 6.8）. The release amounts and proliferation speeds of LAB cells in neutral medium were much larger and faster than those in acidic conditions. Additionally, nxtmobilization of LAB could improve the survival of cells when they were exposed to acidic medium （pH 1.2） with a survival rate of 76%.     

硝酸益康唑微囊化技术研究

以明胶与阿拉伯胶配比为囊材，Span60为乳化剂，戊二醛为交联剂，采用复凝聚法制备了硝酸益康唑微胶囊．并研究了制备工艺条件对微胶囊形态、粒度、药物包封率及释放特性的影响。结果表明，随药物与囊材配比增大．形成的微胶囊数目越多、粒径越小，包封率降低，释放速度增大；戊二醛的用量对微胶囊膜的机械强度、微胶囊形状和粒度及分散性有显著影响。     

温致变色材料微胶囊化研究

温致变色材料的应用通常受强酸性、强碱性及环境杂质污染等因素的影响，以酸碱指示剂和pH值变化的熔化性化合物为变色心材的微胶囊化实验条件，探讨了微胶囊化机理。这类变色材料的微胶囊化技术将促进其应用研究。     

Drying of Micro-Encapsulated Lactic Acid Bacteria—Effects of Trehalose and Immobilization on Cell Survival and Release Properties

Lactic acid bacteria (LAB) were encapsulated with alginate, gelatin and trehalose additives by the extrusion method and dried at 4 ℃. The microcapsules were generally spherical and had a wrinkled surface with a size of 1.7 mm ± 0.2 mm. Trehalose as a carbohydrate source in the culture medium could reduce acid production and performed no function in the positive proliferation of LAB. Using trehalose as a carbohydrate source and protective medium simultaneously had a benefit in the protection of LAB cells dur...