Formation and early development of tetraspores of Polysiphonia urceolata （Rhodomelaceae, Rhodophyta）

Polysiphonia urceolata is one type of potential commercial red seaweeds used for breeding and cultivation, because of its significant biochemical and biomedical application. However, the information of breeding and seedling incubation for cultivation is limited, especially the early development. In this study, tetrasporohyte and gametophyte of P. urceolata were taken as the study materials in Huiquan Bay, Qingdao, China. The cleaned and sterilized tetrasporophytes and gametophytes were pre-cultured in sterilized seawater, then nurtured at 18°C, 25 μmol photons m⁻² s⁻¹ in 12:12 h (light:dark) photoperiod. Continuous observation under microscope showed that the early development consists of bipolar division stage and seedling stage. In the division stage, tetraspores germinate into bipolar sporelings that further differentiate into a colorless rhizoidal portion and a lightly pigmented upright shoot. The lightly pigmented rhizoidal cell develops to a rhizoid and the larger pigmented cell transforms to an erect axis. In the seedling stage, several quasi-protuberances appear on the erect axis and form juvenile seedlings. The results demonstrate the culture of P. urceolata from tetraspores under laboratory conditions. Supported by National Key Technology Support Program, Development Program of China (No.2006AA09Z21), National Natural Science Foundation of China (No. 40618001 and N_CUHK438/06) and Shandong Agricultural Seed Stock Breeding Project     

Early development of Chondrus ocellatus Holm （Gigartinaceae, Rhodophyta）

1 INTRODUCTION Red algae C. ocellatus lives in temperate zone of coastal area, and inhabits at substrates from high tide to low tide regions. Its life history is composed of three phases: tetrasporophyte, gametophyte and carposporophyte. Chondrus is one o…     

Formation and early development of tetraspores of Polysiphonia urceolata (Rhodomelaceae, Rhodophyta)

Polysiphonia urceolata is one type of potential commercial red seaweeds used for breeding and cultivation, because of its significant biochemical and biomedical application. However, the information of breeding and seedling incubation for cultivation is limited, especially the early development. In this study, tetrasporohyte and gametophyte of P. urceolata were taken as the study materials in Huiquan Bay, Qingdao, China. The cleaned and sterilized tetrasporophytes and gametophytes were pre-cultured in sterilized seawater, then nurtured at 18℃, 25 μmol photons m-2 s-1 in 12:12 h (light:dark) photoperiod. Continuous observation under microscope showed that the early development consists of bipolar division stage and seedling stage. In the division stage, tetraspores germinate into bipolar sporelings that further differentiate into a colorless rhizoidal portion and a lightly pigmented upright shoot. The lightly pigmented rhizoidal cell develops to a rhizoid and the larger pigmented cell transforms to an erect axis. In the seedling stage, several quasi-protuberances appear on the erect axis and form juvenile seedlings. The results demonstrate the culture of P. urceolata from tetraspores under laboratory conditions.     

热带产卡拉胶海藻形态及表皮特征比较

2008年6月至2009年6月,在山东青岛实验室和海南陵水试验平台,以光学显微镜及电子扫描显微镜等技术,对12种原产印度尼西亚和菲律宾热带产胶红藻外观形态,表皮细胞特征进行了比较。结果表明：1）不同株系藻体呈现褐色、绿色、黄绿色、翡翠绿色、橄榄绿色和咖啡色等多样性;2）在表皮细胞特性方面,5号株系细胞最小、排列最致密、...     

汞离子对细基江蓠孢子萌发和生长的影响

研究了4种Hg2+浓度对细基江蓠果孢子和四分孢子附着、萌发和生长的影响。结果表明:与对照组相比,Hg2+抑制了果孢子附着却促进了四分孢子的附着,但0.0025 mg/L浓度与对照组的差异不显著(P>0.05),各浓度间差异显著(P<0.05);Hg2+在实验浓度范围内(0.0025、0.0050、0.0075、0.0100 mg/L)影响了孢子的正常萌发,使孢子不能正常分裂和生长,刺激了孢子脱落,并且使盘状体的形态发生变异;`Hg2+不同浓度之间也存在显著差异(P<0.05),并且Hg2+浓度越高对孢子的萌发和生长影响越大。     

热带产卡拉胶海藻形态及表皮特征比较

2008 年6 月至2009 年6 月, 在山东青岛实验室和海南陵水试验平台, 以光学显微镜及电子扫描显微镜等技术, 对12 种原产印度尼西亚和菲律宾热带产胶红藻外观形态, 表皮细胞特征进行了比较。结果表明:1)不同株系藻体呈现褐色、绿色、黄绿色、翡翠绿色、橄榄绿色和咖啡色等多样性; 2)在表皮细胞特性方面, 5 号株系细胞最小、排列最致密、分布最均匀, 8 号株系细胞最大、胞间距最宽; 3)在亚显微结构方面, 1 号藻呈均一球状突起, 2 和4 号藻为不规则突起, 3、6 和9 号藻表面布满酒窝样凹陷, 5和8 号藻表面比较平整, 7 和11 号藻呈脑纹状褶皱, 12 号藻在凹凸结构外覆盖鳞片状白色胶质; 4)在实验株中, 1、2 和4 号藻未发现四分孢子, 3、5、6、11 和12 号藻表皮细胞上具有结构对称的四分孢子, 且主要分布于较老的主干上; 5)在四分孢子排列上, 3、5、11 和12 号四分孢子间排列紧密, 6 号藻四分孢子间距离较远; 6)不同藻的四分孢子囊大小形态存在明显差异, 6 号藻株四分孢子囊最长, 约110 μm, 5号藻四分孢子囊最短, 约40 μm, 3、11、12 号藻四分孢子囊长约60 μm, 四分孢子囊宽度相对恒定, 除5 号藻较窄约为10 μm 外, 其他株系均约为15 μm。     

2,4-二氯苯氧乙酸对细基江蓠孢子生长和存活的影响

以细基江蓠(Gracilaria tenuistipitata)的果孢子和四分孢子为实验材料,用不同浓度的(0,0.1,0.5,1,2,4,6 mg/L)生长激素2,4-二氯苯氧乙酸(2,4-D)处理刚刚放散的孢子和附着2d孢子,研究其对果孢子和四分孢子附着和生长及存活的影响。与对照组相比,2,4-D使两种孢子的附着量明显降低;2.0~4.0mg/L的2,4-D可以极显著的促进孢子的生长(P<0.01);0.1~2.0mg/L的2,4-D提高孢子的存活率(P<0.01);6.0mg/L的2,4-D使得孢子的生长和存活率降低。综合以上指标,在细基江篱采孢子育苗时,孢子附着2~3d后用2,4-D处理孢子萌发体,以1.0~2.0mg/L为宜。