栉孔扇贝foxl2基因在卵子发生中的必要性

采用RNAi技术研究了栉孔扇贝(Chlamysfarreri)foxl2基因在卵子发生和卵巢功能维持中的作用。使用体外合成的栉孔扇贝foxl2双链RNA(dsRNA),以每只50μg/次的剂量注射进闭壳肌中,连续注射2次(第一次注射后7天,再进行第二次注射);以注射相同体积PBS(相同注射方法)的扇贝作为阴性对照组,以不注射扇贝作为空白对照组。qRT-PCR检测干扰组扇贝卵巢中的foxl2 mRNA表达量较空白对照组下降了62%, Western blotting检测该蛋白在卵巢中的含量也明显下降,显示靶基因的表达水平被有效地敲降。组织学观察发现,栉孔扇贝foxl2干扰后的卵巢中卵母细胞形态异常、细胞核固缩,卵子发生明显受阻。表明该基因在栉孔扇贝卵子发生中起重要作用。     

三角帆蚌鳃的组织学研究

对非繁殖期与繁殖前期的雌性三角帆蚌鳃瓣作组织切片,观察其组织形态结构。结果表明,三角帆蚌内、外鳃的鳃丝在组成上是相同的,从外到内,其结构可以分为:上皮、基膜、结缔组织,鳃丝上密布纤毛,鳃丝结缔组织中含吞噬细胞、透明细胞等。外鳃具特有结构——瓣间隔和育儿囊,且在瓣间隔两侧,有发达的小水管系统。由于这种结构的存在,使得内外鳃瓣在鳃瓣厚度、鳃小瓣厚度、鳃丝宽度上存在显著性差异。繁殖前期,内鳃基本无变化,而外鳃发生明显的变化,鳃瓣增厚,鳃腔扩大形成孵化室,为胚胎发育提供一个良好的发育环境。     

苯并[a]芘对雄性褐菖鲉精巢成熟的影响

本实验采用雄性褐菖鲉作为实验对象,腹腔注射0.5×10-6、1×10-6、5×10-6、10×10-6(m/m)苯并[a]芘,7d后苯并[a]芘处理组精巢睾酮水平和睾酮与17β-雌二醇的比值都有上升,但都未表现出显著性差异(P>0.05).腹腔注射50×10-6(m/m)苯并[a]芘,11d后取出精巢,常规石蜡切片,结果显示雄性褐菖鲉的精巢,精小叶腔和输出管中精子密集程度明显增加,次级精母细胞减少,精巢壁厚度变薄.结果提示,苯并[a]芘对褐菖鲉具有类雄激素效应.     

卵形鲳鲹早期卵子发生显微及超微结构的研究

本文研究了卵形鲳鲹早期卵子发生的显微与超微结构特点。研究结果表明在1～2龄鱼卵巢中卵原细胞进入首次成熟分裂前期的结构特点，3～4龄鱼才开始进入小生长期，文中讨论了此鱼性腺成熟的年龄以及如何加速性腺发育，为人工繁殖提供科学依据。     

缢蛏消化道组织学与组织化学的研究

运用组织学和组织化学染色方法对缢蛏消化道的组织结构和功能进行了研究。结果表明 ,缢蛏的消化道由唇瓣、口、食道、胃、晶杆囊、肠、直肠和肛门组成。除唇瓣外的消化管壁由内向外可分为 4层 :粘膜层、粘膜下层、肌层和外膜。粘膜层包括粘膜上皮和粘膜肌层。口腔上皮为假复层纤毛柱状上皮 ,其它部位的粘膜上皮都为单层纤毛柱状上皮。胃壁的局部和肠壁缺乏肌层。除口的腹面、直肠、肛门等处的外膜为浆膜外 ,其余皆为纤维膜。肠上皮、直肠上皮和唇瓣上皮有较强的碱性磷酸酶活性 ,胃上皮和肠上皮有较强的酸性磷酸酶活性。直肠的粘膜及粘膜下层中含有丰富的钙和铁     

南海北部斑节对虾卵巢发育的形态及组织学观察

研究了南海北部斑节对虾Penaeus monodon卵巢发育的形态结构及组织学。以卵巢的组织学、性腺成熟指数(GSI)、卵巢的颜色和大小、卵细胞的直径、卵核的大小、形状、核仁的形态分布、不同发育阶段卵细胞数量比例等为参数,对南海北部斑节对虾卵巢发育的6个时期(卵原细胞期、染色质核仁期、周边核仁期、卵黄囊期、成熟期和恢复期)的组织结构特征进行了研究。并与菲律宾野生斑节对虾种群的卵巢组织结构特征进行了比较,发现2个地理种群的斑节对虾繁殖力存在一定差异。     

褐菖鲉仔、稚鱼消化系统发育的组织学观察

为探究卵胎生鱼类褐菖鲉(Sebastiscus marmoratus)的仔、稚鱼消化系统组织学特点, 采用常规组织切片技术, 并用HE染色和组织化学染色, 比较分析了产出后0~50d的褐菖鲉仔、稚鱼消化系统发育的组织学变化。结果显示: 褐菖鲉初产仔鱼已分化出口咽腔, 并具有初始的食道、胃、肠、肝脏和胰脏; 2日仔鱼肛门与外界相通, 食道扩大, 开始摄食, 进入混合性营养期; 3~4日仔鱼幽门盲囊出现, 食道内壁出现黏膜皱褶5~6个, 胃黏膜皱褶5个, 肝细胞团区域扩大; 5~6日仔鱼卵黄囊和油球耗尽, 进入外源性营养期, 食道黏膜上皮出现杯状细胞, 肠道弯曲, 可区分前中肠和后肠两部分; 10~14日仔鱼食道环肌层明显, 黏膜皱褶增加到7~12个, 胃壁四层结构基本形成, 肠道黏膜皱褶加深, 纹状缘清晰可见, 肝细胞分裂迅速, 数量显著增加, 体积增大, 出现肝血窦和胰岛; 28~30日稚鱼出现胃腺和胃小凹, 已具功能性胃, 标志着稚鱼期的开始, 此时胰岛细胞数量和酶原颗粒增多, 消化能力显著提高; 47~50日稚鱼已基本具有成鱼胃的特征, 肠道纹状缘发达, 肝细胞呈多边形, 细胞内含大量脂肪颗粒, 消化系统从结构和功能上已趋于完善。由此可见, 褐菖鲉仔鱼和稚鱼消化系统的发育具有卵胎生鱼类发育较早的特点, 与其消化功能的完善密切相关。     

A new titanosaur from the Lower Cretaceous of Brazil

Here we describe a new record of a sauropod dinosaur from the Lower Cretaceous (Hauterivian–Barremian) Rio Piranhas Formation, Sousa Basin, NE Brazil. Dinosaur fossil bones from this deposit were unknown until now. Thus, the discovery of a sauropod fibula from this locality is highly significant. Our discovery represents an indeterminate titanosaur and the earliest stratigraphic occurrence of this group in central Gondwana. When compared to chronocorrelate titanosaur trackmakers of this geological unit, this fossil specimen appears substantially smaller. Histological analysis of the fibula suggests that this is a relatively young individual (approximately 40–50% adult body size) that had passed its most rapid phase of early juvenile growth, but had not yet attained somatic maturity. Thus, the fibula recovered is from a young individual rather than from a small-bodied adult titanosaur.     

Aspects of reproduction of the brown mussel Perna perna at the Iture rocky beach near Cape Coast,Ghana

Aspects of the reproductive biology of the brown mussel Perna perna at the Iture rocky beach near Cape Coast, Ghana, were studied from September 2014 to August 2015. The current study was aimed at providing information useful for managing the mussel fishery in this locality and also that would form the basis for designing appropriate culture methods for the species. Microscopic examination of fresh smears of gonadal material, as well as histological preparations of the gonad, were used to study the sexuality and breeding pattern of the species. Monthly gonadal and condition indices were also determined. Perna perna exhibited gonochoristic sexuality with a sex ratio of approximately 1:1 throughout the study period. Sexes were identifiable at shell lengths of 15.0–19.9 mm. Five stages of gonadal development were identified in both sexes. Gametogenic activity was continuous throughout the year, with two major spawning activities, from April to June and from August to December. These periods coincided with the major and minor rainy seasons, respectively, as well as the major upwelling period in August. Condition indices suggest that the mussels were in better condition for harvesting in March and August prior to the major spawning events.     

Histological and Histochemical Analyses of the Cuttlebone Sac of the Golden Cuttlefish Sepia esculenta

The secretion function of mantle is closely related to shell formation in some bivalves and gastropods. Up to now, few researches have been reported for cuttlebone formation in the class Cephalopoda. In this study, the structure and secretion function of cuttlebone sac of the golden cuttlefish Sepia esculenta was analyzed using the histological and histochemical methods. The results showed that high and columnar cells located in sac epithelium, and flat cells existed near the base membrane. A lot of fibroblasts were found in the lateral mantle collective tissue. Some mucus, mucopolysaccharide and alkaline phosphatase (ALP) were found in the sac. The ultrastructural characteristics of Quasi-connective-tissue-calcium cells (QCTCC) were observed using a transmission electron microscope (TEM). The relationship between cuttlebone sac secretion function and shell formation was discussed.