广盐性鱼类渗透压适应性与生理可塑性机制研究

拥有强大的渗透压调节能力对广盐性鱼类的生存至关重要。目前,关于鱼类渗透压调节机制已有不少研究,但均存在较大的局限性。本文从广盐性鱼类渗透压信号转导机制、渗透胁迫的细胞调控机制、渗透调控的内分泌调节机制和无机离子通道和转运蛋白介导的渗透调控等方面对广盐性鱼类的渗透压适应性和生理可塑性机制进行分析,以期从分子、细胞、通路和生理等层次初步探索广盐性鱼类盐度胁迫后的可塑性表型变化和不同盐度条件下的应答机制,为广盐性鱼类的渗透压调节机制的深入研究奠定基础。对广盐性鱼类渗透压适应性与生理可塑性机制研究,有助于研究其环境适应机理,促进野生鱼种的人工化养殖以及新品种的育种从而提高经济效益、社会效益和生态效益,对促进水产养殖学进步以及养殖业的发展具有重要意义,同时,为研究广盐性鱼类的渗透压调节机制开辟新的研究方向。     

Revisiting the existence of an effective stress for wet granular soils with micromechanics

A possible effective stress variable for wet granular materials is numerically investigated based on an adapted discrete element method (DEM) model for an ideal three‐phase system. The DEM simulations consider granular materials made of nearly monodisperse spherical particles, in the pendular regime with the pore fluid mixture consisting of distinct water menisci bridging particle pairs. The contact force‐related stress contribution to the total stresses is isolated and tested as the effective stress candidate for dense or loose systems. It is first recalled that this contact stress tensor is indeed an adequate effective stress that describes stress limit states of wet samples with the same Mohr‐Coulomb criterion associated with their dry counterparts. As for constitutive relationships, it is demonstrated that the contact stress tensor used in conjunction with dry constitutive relations does describe the strains of wet samples during an initial strain regime but not beyond. Outside this so‐called quasi‐static strain regime, whose extent is much greater for dense than loose materials, dramatic changes in the contact network prevent macroscale contact stress‐strain relationships to apply in the same manner to dry and unsaturated conditions. The presented numerical results also reveal unexpected constitutive bifurcations for the loose material, related to stick‐slip macrobehavior.     

A multiscale work-analysis approach for geotechnical structures

This paper presents a second-order work analysis in application to geotechnical problems by using a novel effective multiscale approach. To abandon complicated equations involved in conventional phenomenological models, this multiscale approach employs a micromechanically-based formulation, in which only four parameters are involved. The multiscale approach makes it possible a coupling of the finite element method (FEM) and the micromechanically-based model. The FEM is used to solve the boundary value problem (BVP) while the micromechanically-based model is utilized at the Gauss point of the FEM. Then, the multiscale approach is used to simulate a three-dimensional triaxial test and a plain-strain footing. On the basis of the simulations, material instabilities are analyzed at both mesoscale and global scale. The second-order work criterion is then used to analyze the numerical results. It opens a road to interpret and understand the micromechanisms hiding behind the occurrence of failure in geotechnical issues.     

A continuum‐discrete hydromechanical analysis of granular deposit liquefaction

A coupled continuum‐discrete hydromechanical model was employed to analyse the liquefaction of a saturated loose deposit of cohesionless particles when subjected to a dynamic base excitation. The pore fluid flow was idealized using averaged Navier–Stokes equations and the discrete element method was employed to model the solid phase particles. A well established semi‐empirical relationship was utilized to quantify the fluid–particle interactions. The conducted simulations revealed a number of salient micro‐mechanical mechanisms and response patterns associated with the deposit liquefaction. Space and time variation of porosity was a major factor which affected the coupled response of the solid and fluid phases. Pore fluid flow was within Darcy's regime. The predicted response exhibited macroscopic patterns consistent with experimental results and case histories of the liquefaction of granular soil deposits. Copyright © 2004 John Wiley & Sons, Ltd.     

Reliability assessment of granular filters in embankment dams

Empirical criteria have been used successfully to design filters of most embankment large dam projects throughout the world. However, these empirical rules are only applicable to a particular range of soils tested in laboratory and do not take into account the variability of the base material and filter particle sizes. In addition, it is widely accepted that the safety of fill dams is mainly dependent on the reliability of their filter performance. The work herein presented consists in a new general method for assessing the probability of fulfilling any empirical filter design criteria accounting for base and filter heterogeneity by means of first‐order reliability methods (FORM), so that reliability indexes and probabilities of fulfilling any particular criteria are obtained. This method will allow engineers to estimate the safety of existing filters in terms of probability of fulfilling their design criteria and might also be used as a decision tool on sampling needs and material size tolerances during construction. In addition, sensitivity analysis makes possible to analyse how reliabilities are influenced by different sources of input data. Finally, in case of a portfolio risk assessment, this method will allow engineers to compare the safety of several existing dams in order to prioritize safety investments and it is expected to be a very useful tool to evaluate probabilities of failure due to internal erosion. Copyright © 2006 John Wiley & Sons, Ltd.     

MORPHOLOGICAL AND FUNCTIONAL STUDIES ON THE EPIDERMAL CELLS OF AMPHIOXUS (BRANCHIOSTOMA BELCHERI TSINGTAUENSE) AT DIFFERENT DEVELOPMENTAL STAGES

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Readout Techniques for Drift and Low Frequency Noise Rejection in Infrared Arrays

Three different methods are presented to subtract thermal drifts and low-frequency noise from the signal of infrared array. The first is dead pixels with open Indium bumps, the second is reference output as implemented on the Hawaii2 multiplexer, and the third is dark pixels to emulate reference cells having a capacity connected to the gate of the unit cell field-effect transistor (FET). The third method is the most effective and yields a reduction in readout noise from15.4–9.4 erms. A novel method will be described to extend this readout technique to the Aladdin 1 K × 1 K InSb array. This revised version was published online in July 2006 with corrections to the Cover Date.     

CHARACTERIZATION OF LACTATE DEHYDROGENASE ISOZYME PATTERN AND MORPHOLOGY OF THREE MARINE FISH CELL LINES

Three continuous marine fish cell lines of FG (i. e., Hounder Gill) from flounder (Paralichthys olivaceus) gill, SPH (i. e. , Sea Perch Heart) from sea perch (Lateolabrax japonicus) heart and RSBF (i.e., Red Sea Bream Fin) from red sea bream (Pagrosomus major) fin, were characterized by lactate dehydrogenase (LDH) isozyme and morphological analysis. The LDH isozyme patterns of these three cell lines and their corresponding tissues of origin were investigated and compared. The results showed: (1) No difference was found in the LDH isozyme patterns of FG and flounder gill tissue. However, the LDH isozyme patterns of SPH and RSBF were significantly different from their corresponding tissues of origin; (2) LDH isozyme patterns of FG, SPH and RSBF were markedly different from each other and could serve as genetic markers for species identification and detection of cross contamination. Morphological change analysis of these three cell lines in comparison to their original tissues indicated that FG cells still appeared epithelioid without morphological transformation. However, morphological changes were found in SPH and RSBF compared to their original tissues. Therefore, the cellular morphology was still plastic in the relatively stable culture conditions, and it was possible that change of LDH patterns wasrelated to morphological changes of fish cells in vitro.     

Biaxial test simulations using a packing of polygonal particles

The mechanical response of cohesionless granular materials under monotonic loading is studied by performing molecular dynamic simulations. The diversity of shapes of soil grains is modelled by using randomly generated convex polygons as granular particles. Results of the biaxial test obtained for dense and loose media show that samples achieve the same void ratio at large strains independent of their initial density state. This limit state resembles the so‐called critical state of soil mechanics, except for some stress fluctuations, which remain for large deformations. These fluctuations are studied at the micro‐mechanical level, by following the evolution of the co‐ordination number, force chains and the fraction of the sliding contacts of the sample. Copyright © 2007 John Wiley & Sons, Ltd.