Evaluating the effects of catch-and-release angling on Cape stumpnose Rhabdosargus holubi in a South African estuary

Fisheries managers are increasingly promoting catch-and-release (C&R) to manage recreationally angled fish stocks. Despite this, there is a scarcity of information on the effects of C&R on estuarine-dependent species. Cape stumpnose Rhabdosargus holubi dominates the recreational fisheries catch and provides an important source of food for subsistence fishers in some temperate South African estuaries. The health and survival of R. holubi exposed to a C&R event was investigated by examining their physiological stress response (blood glucose and lactate), reflex impairment (reflex action mortality predictors [RAMP]) and short-term (12-hour) survival. Fish were captured and exposed to one of three air-exposure treatments: 0 s, 30 s or 90 s. Stress and health were measured either immediately (immediate) or one hour after (delayed) the C&R event. There was no significant difference in blood glucose between air-exposure treatments, but there was a significant difference between the mean immediate and delayed glucose levels within each treatment (F_(2,143) = 81.8, p < 0.01). In contrast, blood lactate level was significantly higher in the 90-s treatment (p < 0.05). Immediate blood lactate levels were significantly lower than the delayed samples for each treatment (F = 4.29, p = 0.02; n = 169). Although all fish exhibited at least one reflex impairment, the RAMP score was significantly higher in the 90-s air-exposure treatment (H_(2,86) = 9.73, p = 0.007). Also, RAMP scores were significantly lower in the delayed samples (p < 0.01). Although short-term mortality was relatively low (2.3%) for this species, it was highest in the 90-s treatment (7%). These results suggest that physiological stress is higher when R. holubi are exposed to longer periods of air exposure and that the physiological stress of fish subject to a C&R event is best measured after a delay.     

广盐性鱼类渗透压适应性与生理可塑性机制研究

拥有强大的渗透压调节能力对广盐性鱼类的生存至关重要。目前,关于鱼类渗透压调节机制已有不少研究,但均存在较大的局限性。本文从广盐性鱼类渗透压信号转导机制、渗透胁迫的细胞调控机制、渗透调控的内分泌调节机制和无机离子通道和转运蛋白介导的渗透调控等方面对广盐性鱼类的渗透压适应性和生理可塑性机制进行分析,以期从分子、细胞、通路和生理等层次初步探索广盐性鱼类盐度胁迫后的可塑性表型变化和不同盐度条件下的应答机制,为广盐性鱼类的渗透压调节机制的深入研究奠定基础。对广盐性鱼类渗透压适应性与生理可塑性机制研究,有助于研究其环境适应机理,促进野生鱼种的人工化养殖以及新品种的育种从而提高经济效益、社会效益和生态效益,对促进水产养殖学进步以及养殖业的发展具有重要意义,同时,为研究广盐性鱼类的渗透压调节机制开辟新的研究方向。     

解郁安神法联合体外反搏对PCI术后气滞血瘀型胸痹患者的临床疗效观察

目的：观察解郁安神法联合体外反搏（EECP）对经皮冠状动脉介入治疗（PCI）术后气滞血瘀型胸痹患者的临床疗效。方法：将80例冠心病PCI术后气滞血瘀型胸痹患者随机分为治疗组和对照组,每组各40例。对照组采用PCI术后规范化治疗,同时联合EECP治疗;治疗组在对照组基础上加服解郁安神方治疗,随访观察7周,比较2组临床证候疗效、Zung焦虑自评量表（SAS）评分、抑郁自评量表（SDS）评分、西雅图心绞痛调查量表（SAQ）评分。结果：总有效率治疗组为82.5%（33/40）,高于对照组的60.0%（24/40）,2组比较,差异有统计学意义（P<0.05）;2组SAS、SDS、SAQ评分治疗前后组内比较及治疗后组间比较,差异均有统计学意义（P<0.05）。结论：解郁安神法联合EECP治疗可明显改善PCI术后气滞血瘀型胸痹患者的临床症状,缓解焦虑抑郁程度,提高患者生活质量,值得临床推广应用。     

MORPHOLOGICAL AND FUNCTIONAL STUDIES ON THE EPIDERMAL CELLS OF AMPHIOXUS (BRANCHIOSTOMA BELCHERI TSINGTAUENSE) AT DIFFERENT DEVELOPMENTAL STAGES

ImODUcrroNTheVertchrateepidennisisinintirnatecontaCtwiththeenvironmentandre~environmentaladaptions.TheepidermisofterreStrialanbolsiskeratinedwhilethatofamPhibiousandaquaticfonnsisrelataltomucus-produCti0n.Theepidennisadsasafi-nalbatherbetweentheorganismanditsenvironmentandoneofitSrnainfunCtionsistoproteCttheorganismagainstextemalpathogens.lnadditiontoIneCanicalproedonofthebody,theepidertnisofamPhibiansandfishescansededinsandotherantibacterialpeptidesthathelpinthebonedefenSeoftheanhal(Su…     

Readout Techniques for Drift and Low Frequency Noise Rejection in Infrared Arrays

Three different methods are presented to subtract thermal drifts and low-frequency noise from the signal of infrared array. The first is dead pixels with open Indium bumps, the second is reference output as implemented on the Hawaii2 multiplexer, and the third is dark pixels to emulate reference cells having a capacity connected to the gate of the unit cell field-effect transistor (FET). The third method is the most effective and yields a reduction in readout noise from15.4–9.4 erms. A novel method will be described to extend this readout technique to the Aladdin 1 K × 1 K InSb array. This revised version was published online in July 2006 with corrections to the Cover Date.     

CHARACTERIZATION OF LACTATE DEHYDROGENASE ISOZYME PATTERN AND MORPHOLOGY OF THREE MARINE FISH CELL LINES

Three continuous marine fish cell lines of FG (i. e., Hounder Gill) from flounder (Paralichthys olivaceus) gill, SPH (i. e. , Sea Perch Heart) from sea perch (Lateolabrax japonicus) heart and RSBF (i.e., Red Sea Bream Fin) from red sea bream (Pagrosomus major) fin, were characterized by lactate dehydrogenase (LDH) isozyme and morphological analysis. The LDH isozyme patterns of these three cell lines and their corresponding tissues of origin were investigated and compared. The results showed: (1) No difference was found in the LDH isozyme patterns of FG and flounder gill tissue. However, the LDH isozyme patterns of SPH and RSBF were significantly different from their corresponding tissues of origin; (2) LDH isozyme patterns of FG, SPH and RSBF were markedly different from each other and could serve as genetic markers for species identification and detection of cross contamination. Morphological change analysis of these three cell lines in comparison to their original tissues indicated that FG cells still appeared epithelioid without morphological transformation. However, morphological changes were found in SPH and RSBF compared to their original tissues. Therefore, the cellular morphology was still plastic in the relatively stable culture conditions, and it was possible that change of LDH patterns wasrelated to morphological changes of fish cells in vitro.     

中国对虾血细胞酶细胞化学的初步研究

运用电镜酶细胞化学技术 ,对中国对虾 (Penaeus chinensis)的血细胞和血清中的酚氧化酶、酸性磷酸酶、碱性磷酸酶和过氧化物酶的酶活性部位进行标记。实验结果显示 :酚氧化酶主要以酶原形式存在于大颗粒细胞的颗粒体中 ,酸性磷酸酶和碱性磷酸酶以酶原形式存在于小颗粒细胞的颗粒体中。健康虾和患病虾血细胞中酶活性的表现形式不尽相同 ,在病理情况下 ,颗粒细胞能以细胞解体的方式 ,排出细胞内的颗粒 ,转化成有活性的酶 ,以此提高血淋巴中这几种酶的活性。透明细胞可以吞噬排放颗粒后的颗粒细胞 ,透明细胞中由高尔基体合成的酸性磷酸酶等溶酶体酶主要用于透明细胞的细胞内消化作用。研究认为 :3种血细胞因含有不同的免疫酶以及酶的作用方式不同而具有不同的免疫功能     

基于生物信息学探讨骨质疏松症与2型糖尿病的关系

目的：基于生物信息学探讨骨质疏松症与 2 型糖尿病的关系。方法：通过基因表达汇编（GEO）数据库检索骨质疏松症和2型糖尿病的相关数据集,并筛选骨质疏松症-2型糖尿病共同差异表达基因,将数据导入STRING数据库找寻关键基因,以一个单细胞测序数据集作为检验集验证关键基因细胞层面的表达水平,对筛选结果进行基因本体（GO）功能及京都基因与基因组百科全书（KEGG）通路富集分析,并对关键靶点、相关中药进行频数统计及功效分类。结果：骨质疏松症与 2 型糖尿病相关通路集中在 ErbB信号通路、丝活化蛋白激酶（MAPK）信号通路、神经营养素信号通路、NOD样受体信号通路。频数统计结果显示,与骨质疏松症-2型糖尿病关键基因相关的中药有51味,以功效分类,可分为清热解毒药、止痛药、祛风药、补虚药、止呕药。结论：骨质疏松症和2型糖尿病都是复杂的代谢性疾病,涉及众多基因表达网络和信号通路。尽管两种疾病存在复杂的基因网络背景,但是分析结果表明两种疾病之间仍存在高度重合的差异基因表达和信号通路。这表明两种疾病的分子机制密切相关,可能存在共同调控的靶点,因此这些靶点可能成为药物同时干预两种疾病的治疗靶点。     

顶空气相色谱法测定海水二甲基硫和浮游植物细胞二甲基硫丙酸的研究

建立了顶空ＧＣ／ＦＰＤ测定海水中二甲基硫（ＤＭＳ）和浮游植物细胞中二甲基硫丙酸（ＤＭＳＰ）的方法，并研究盐度、温度、气液相比ＤＭＳ诸因素对ＤＭＳ顶空灵敏度的影响。该法对ＤＭＳ测定的相对标准偏差均小于６％，平均回收率为１０６％，最低检出限为２０ｎｇ／Ｌ。细胞ＤＭＳＰ先经碱作用转化为ＤＭＳ，在５０℃下作用时间不少于６ｈ，峰高与浓度的双对数线性相关系数大于０．９９。对１９９４年冬、１９９５年夏采自胶州湾     

文昌鱼哈氏窝匀浆使蟾蜍足细胞释精的超微结构研究

本文报道了文昌鱼哈氏窝匀浆刺激蟾蜍足细胞释放精子过程所发生的超微结构变化,进一步证实这种匀浆含有类似脊椎动物促黄体生成素活性物质。