蓝非鲫肠道上皮组织的扫描电镜观察

消消化化道道黏黏膜膜上上皮皮组组织织是是吸吸收收和和同同化化营营养养物物质质的的 主主要要器器官官 , ,在在鱼鱼类类生生长长和和发发育育的的过过程程中中发发挥挥着着极极其其重重 要要的的作作用用 , ,研研究究鱼鱼类类消消化化系系统统有有利利于于深深入入探探讨讨其其食食性性 及及消消化化生生理理学学特特征征 , ,对对于于提提高高经经济济鱼鱼类类的的养养殖殖产产量量和和 营营养养附附加加值值 , ,促促进进养养殖殖环环境境开开发发具具有有重重要要意意义义。。近近年年 来来 , ,有有关关鱼鱼类类消消化化…     

Metallothionein in intestine of red mullet, Mullus barbatus as a biomarker of copper exposure in the coastal marine areas

Dietary metal exposure in fish is studied in native red mullet, Mullus barbatus, involving metallothionein (MT) response in the intestine, a primary site for metal uptake. Intestinal MTs (microg mg(-1) proteins), total proteins (mgml(-1) S50), Cu, Zn, Fe, Mn and Cd (microg ml(-1) S50) are considered as indicators of metal exposure in specimens from near-shore and off-shore locations of the Eastern Adriatic Sea. Metallothionein and Cu are statistically significantly higher in 68 specimens from the Kastela Bay, a near-shore area (30.0+/-6.71microg MT mg(-1) proteins; 0.30+/-0.08microg Cu ml(-1) S50), in comparison to 51 specimens from off-shore area, Solta Island (25.9+/-4.21microg MT mg(-1) proteins; 0.26+/-0.09microg Cu ml(-1) S50). Cytosolic Cu, a MT inducer, is significantly correlated (p<0.05, r=0.44) with MT in specimens from the Kastela Bay, independent on fish biometry. Our study indicates that cytosolic intestinal Cu and MT represent a measurable biochemical response in red mullet specimens, dwelling above sediments contaminated with Cu.     

泥螺消化道内的细菌学分析

泥螺(Ｂｕｌｌａｃｔａｅｘａｒａｔａ)隶属于软体动物门,腹足纲 ,后鳃亚纲,头盾目 ,阿地螺科。其味道鲜美,具有较高的营养价值和经济价值 ,是浙江沿海重要的滩涂养殖品种,至２０００年全省养殖面积已达约１１３００ｈａ。泥螺的生长是以不断吞食周围滩涂中的涂泥和底栖藻类等为营养基础 ,已知滩涂沉积物及动植物碎屑中所含有的微生物从事着大量的转化和分解工作［４］,它们在泥螺体内又会怎样呢 ?为探索泥螺的消化生理功能及与养殖、病害和防治的关系,本文从泥螺消化道内的细菌菌群组成与功能研究入手作初步分析。关于贝类体内的细菌学调查…     

刺参肠道与养殖池塘环境中异养细菌和弧菌数量周年变化

2008年9月~2009年10月,对刺参肠道与养殖环境中异养细菌和弧菌周年变化进行了初步研究。结果表明:刺参肠道与养殖环境中(水体、底泥、附着基)异养细菌数(HPC)、弧菌数(VBC)均呈现出明显的季节变化,肠道中HPC、VBC分别为1.85×105~2.17×109CFU/g、4.1×104~2.2×108CFU/g,水体中HPC、VBC分别为90~4.67×105CFU/mL、0~5.3×103CFU/mL,底泥中HPC、VBC分别为9.80×104~6.72×106CFU/g、1.01×104~5.75×105CFU/g,附着基中HPC、VBC分别为2.78×105~2.57×107CFU/g、4.6×104~1.31×107CFU/g,夏、秋两季细菌总数较高,冬、春低温季节细菌数量显著下降。通过VBC与HPC的比值可以看出,在冬、春季细菌总数较低的季节,弧菌与异养细菌数量比例较高,最高值达到:43.8%、43.2%,通过对优势菌的分离鉴定,冬、春两季灿烂弧菌(Vibrio splendidus)均占优势,这可能是造成冬、春两季刺参发病的诱因之一。本文通过研究刺参肠道与养殖环境中异养细菌和弧菌的变化规律,以期为刺参的健康养殖提供有益参考。