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1.
催化光度法测定痕量锇 总被引:3,自引:0,他引:3
在05mol/L的H2SO4介质中,As(Ⅲ)存在下,Os(Ⅷ)催化KClO3氧化邻苯二酚紫褪色反应。反应受温度、反应时间影响,冷却及加入抗坏血酸可终止反应。据此,建立了一个测定痕量Os(Ⅷ)的新方法。方法的灵敏度为257×10-10g/LOs(Ⅷ),测定范围为0~0091μg/L,用地质管理样中锇的测定验证,结果与原结果相符 相似文献
2.
Molecularly imprinted polymers(MIPs) were prepared by the bulk polymerization using crystal violet as the template molecule, and the methacrylic acid and ethylene glycol dimetheacrylate as functional monomer and cross-linker, respectively. Systematic investigations of synthetic conditions were conducted. The surface morphology and recognition mechanism of the obtained polymers were studied using scanning electron microscope and spectrophotometric analysis. MIPs showed high affinity to template molecule and were successfully applied as special solid-phase extraction sorbent for selective extraction of crystal violet from natural seawater. An off-line molecularly imprinted solid-phase extraction(MISPE) method followed by high-performance liquid chromatography with diodearray detection for the analysis of crystal violet was also established. MISPE columns have good recoveries for crystal violet standard solutions and good linearity was obtained over the concentration range of 0-200 μg L-1(R2 0.99). Finally, two natural seawater samples were investigated. The recoveries of spiked seawater on the MISPE columns were from 44.47% to 62.34%, the relative standard deviation(n=3) being in the range of 2.89%-5.96%. 相似文献
3.
Priyanka Lal Vishnu Agarwal Parul Pruthi Swaranjit Singh Cameotra Vikas Pruthi 《洁净——土壤、空气、水》2008,36(12):963-968
The microbiological assessment of biofilm formation from paper mill effluent discharged through a pipeline revealed a maximum microbial count for Pseudomonas (5·106 cfu/mL) followed by Staphylococcus (4·106 cfu/mL), Bacillus (8.2·105 cfu/mL), Burkholderia (7.2·105 cfu/mL), Enterobacter (5.3·104 cfu/mL), Acinetobacter (4.1·103 cfu/mL), Alcaligenes (1.2·102 cfu/mL) and Klebsiella (0.8·102 cfu/mL) species. Among these species, the maximum biofilm formation was observed after 24 h of incubation by Pseudomonas sp. using a crystal violet (CV) assay. This isolate was later identified by 16S rRNA amplification to be Pseudomonas aeruginosa PME1. Extracellular polymeric substances (EPS) of P. aeruginosa PME1 in the biofilm showed a reduction in total carbohydrate content (42%) with increased protein (9.0%), hexosamine (3.0%) and uronic acid (1.7%) content as compared to its planktonic form. Antimicrobial susceptibility testing revealed that P. aeruginosa PME1 biofilms were 17, 24, 27, 30, and 32 times more resistant to cefotaxime, imipenem, ceftazidime, tazobactam and piperacillin, respectively, than their free flowing counterparts. 相似文献
4.
液相色谱法测定鱼肌肉中孔雀石绿、结晶紫及其代谢物 总被引:11,自引:0,他引:11
孔雀石绿(MG)和结晶紫(GV)曾作为渔药使用,在鱼体内可分别代谢为无色孔雀石绿(LMG)及无色结晶紫(LGV),具有潜在的致癌性,已被许多国家禁用。本文研究了同时检测孔雀石绿、结晶紫及其代谢物无色孔雀石绿和无色结晶紫的液相色谱方法,建立了检测前处理方法及色谱条件。经过液液萃取和丙基磺酸阳离子树脂纯化,采用C18分离柱后串联二氧化铅柱氧化衍生的方法,同时检测母体药物及其代谢物,回收率>70%,重复性的相对标准偏差<15%,定量限分别为2μg/kg及1μg/kg。经样品实测,在一份鲈鱼样品中检测到无色孔雀石绿残留97μg/kg。 相似文献
5.
An optical chemical sensor (optode) is proposed for the kinetic determination of nitrite ion. The optode was fabricated by the immobilization of methyl violet on a triacetylcellulose polymeric membrane. Methyl violet is covalently bonded to a transparent triacetylcellulose film. By immersion of the sensor into an acidic nitrite solution, the absorbance of the sensor at 596 nm decreases with time that is due to the reaction of nitrite with the immobilized methyl violet. A fixed time method of 15 min was used to monitor the reaction. The linear range for the determination of nitrite was 0.20–8.00 µg mL?1 and the limit of detection was 0.08 µg mL?1. The optodes were one‐shot, they had durability more than 2 months and were easily prepared. The optode was successfully applied to the determination of nitrite ion in spring water and sewage samples. 相似文献
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7.
Gopalakrishnan Saranya Panneerselvam Saravanan Mahendradas Dharmendira Kumar Sahadevan Renganathan 《洁净——土壤、空气、水》2011,39(3):283-288
The percentage removal and uptake capacity of Basic Violet 14 using Hydrilla verticillata with living biomass was studied under batch conditions. The survival of H. verticillata was studied using the chlorophyll content in the living biomass. Bioaccumulation of Basic Violet 14 using H. verticillata was tested by varying the wet sorbent dosage (0.5–2.5 g), initial pH (3–8), and initial dye concentrations (5–25 mg L?1). The results show that the plant was effectively accumulating the Basic Violet 14 dye. The uptake capacity of Basic Violet 14 dye was observed as 5.9 and 21.3 mg g?1 at the initial dye concentration of 5 and 25 mg L?1, respectively, for a biomass of 5 g L?1 (wet weight) at pH 7.0 for 144 h. In general, the plant growth was found to be normal at lower concentrations and showed higher removal efficiency. It was also observed that removal efficiency of H. verticillata was found to decrease with increase in initial dye concentration. The biomass sample surface was analyzed using SEM imaging and functional groups present in the biomass were analyzed using FTIR. The equilibrium uptake capacity was analyzed by Langmuir and Freundlich isotherms. The equilibrium data was found to be fit well to both Langmuir and Freundlich isotherm models with higher coefficient of determination. 相似文献
8.
铊—硫氰酸盐—乙基紫高灵敏显色反应及应用研究 总被引:2,自引:0,他引:2
在聚乙烯醇(PVA)存在下,Tl(SCN)_4~-与乙基紫形成离子缔合物。适宜酸度为0.12—0.36mol/L H_2SO_4,λ_(max)为560 nm,ε为1.12×10~6L·mol~(-1)·cm~(-1),Tl在0—10μg/25 ml范围服从比耳定律。考察了40多种共存物质影响,大多数元素不干扰。方法简单、快速,已用于某些岩矿中Tl的测定。 相似文献
9.
钼—邻苯二酚紫多元配合物协同增敏显色的研究 总被引:1,自引:0,他引:1
研究了在pH4~6的弱酸性介质中,羟胺(HY)和溴化十二烷基吡啶(DPB)在钼、邻苯二酚紫(PV)配合物中的增敏作用和显色条件。有色配合物的最大吸收峰为718nm,表观摩尔吸光系数达1.2×106L·mol-1·cm-1,钼的检出限为0.6μg/L,反应在室温下瞬间完成,方法的线性范围在0.8~100μg/LMo,对w(Mo)在10-3水平的试样测定4次,RSD为4.6%~6.2%。对企业标准钢样的测定结果与推荐值相符。 相似文献
10.
废水中阴离子表面活性剂十二烷基磺酸钠的测定 总被引:5,自引:0,他引:5
应用物质的吸收光谱特性,研究了不同的条件下结晶紫与阴离子表面活性剂十二烷基磺酸钠形成的紫色离子缔合物的光学特性。发现室温条件下,在pH7.00的缓冲溶液时,此紫色离子缔合物在580nm处有最大吸收值,十二烷基磺酸钠在0~2.0mg/L范围内回收率为96%~103%,且RSD<3.7%。据此建立了分光光度法测定阴离子表面活性剂的方法,此法已用于水体样品中阴离子表面活性剂的测定,获得满意的结果。 相似文献