不同盐度对文蛤(Meretrix meretrix)呼吸代谢及体内酶活性的影响

为研究不同盐度对文蛤呼吸代谢的影响,本实验设置5个盐度(‰)梯度(11、18、25、32、39),检测不同盐度对文蛤(Meretrixmeretrix)耗氧和排氨的影响,以及文蛤的外套膜、鳃、肝胰腺三种组织中乳酸脱氢酶和Na+/K+-ATP酶活性的变化。结果表明:随着盐度的不断升高,文蛤耗氧率先升后降再升,在盐度18时达到最大值;排氨率先升后降,在盐度32时达到最大值。随着盐度不断升高和胁迫时间延长,文蛤的肝胰腺中乳酸脱氢酶活力总体呈先升高后下降再升高的趋势(P0.05),酶活力在盐度39时为最高;随着盐度不断升高和胁迫时间延长,文蛤的外套膜中Na+/K+-ATP酶活力总体呈先下降再升高后下降的趋势(P0.05),在盐度32时为最高;文蛤的外套膜和鳃中乳酸脱氢酶活力以及鳃和肝胰腺中Na+/K+-ATP酶活力受盐度影响不显著(P0.05),酶活力变化也多呈现"W"形的变化趋势。研究结果为文蛤的人工养殖提供参考。     

Modelling the effect of changing precipitation inputs on deep soil water utilization

Forests in the Southeastern United States are predicted to experience future changes in seasonal patterns of precipitation inputs as well as more variable precipitation events. These climate change‐induced alterations could increase drought and lower soil water availability. Drought could alter rooting patterns and increase the importance of deep roots that access subsurface water resources. To address plant response to drought in both deep rooting and soil water utilization as well as soil drainage, we utilize a throughfall reduction experiment in a loblolly pine plantation of the Southeastern United States to calibrate and validate a hydrological model. The model was accurately calibrated against field measured soil moisture data under ambient rainfall and validated using 30% throughfall reduction data. Using this model, we then tested these scenarios: (a) evenly reduced precipitation; (b) less precipitation in summer, more in winter; (c) same total amount of precipitation with less frequent but heavier storms; and (d) shallower rooting depth under the above 3 scenarios. When less precipitation was received, drainage decreased proportionally much faster than evapotranspiration implying plants will acquire water first to the detriment of drainage. When precipitation was reduced by more than 30%, plants relied on stored soil water to satisfy evapotranspiration suggesting 30% may be a threshold that if sustained over the long term would deplete plant available soil water. Under the third scenario, evapotranspiration and drainage decreased, whereas surface run‐off increased. Changes in root biomass measured before and 4 years after the throughfall reduction experiment were not detected among treatments. Model simulations, however, indicated gains in evapotranspiration with deeper roots under evenly reduced precipitation and seasonal precipitation redistribution scenarios but not when precipitation frequency was adjusted. Deep soil and deep rooting can provide an important buffer capacity when precipitation alone cannot satisfy the evapotranspirational demand of forests. How this buffering capacity will persist in the face of changing precipitation inputs, however, will depend less on seasonal redistribution than on the magnitude of reductions and changes in rainfall frequency.     

A-FPN算法及其在遥感图像船舶检测中的应用

光学遥感图像船舶检测主要面临两个挑战:光学遥感图像背景复杂,船舶检测易受海浪、云雾及陆地建筑等多方面干扰;遥感图像分辨率低,船舶目标小,对于其分类与定位带来很大困难;针对上述问题,在FPN的基础上,提出一种融入显著性特征的卷积神经网络模型A-FPN (Attention-Based Feature Pyramid Networks)。首先,利用卷积提取图像特征金字塔;然后,利用顶层金字塔逐级构建显著特征层,抑制背景信息,通过金字塔顶层的细粒度特征提高浅层特征的表达能力,构建自上而下的多级显著特征映射结构;最后利用Softmax分类器进行多层级船舶检测。A-FPN模型利用显著性机制引导不同感受下的特征进行融合,提高了模型的分辨能力,对遥感图像处理领域具有重要应用价值。实验阶段,利用公开的遥感目标检测数据集NWPU VHR-10中的船舶样本进行测试,准确率为92.8%,表明A-FPN模型适用于遥感图像船舶检测。     

复杂地形景区三维导览图的设计与实现

为满足复杂地形景区对三维地图导览的需求,构建更为清晰的三维地图模型,提供良好的三维导览地图设计方案显得尤为重要。为了更好地与卫星影像进行贴合,DEM数据的采样密度要与卫星影像的分辨率一致。本文通过对比当下常用的四种空间插值方法的适用范围与运用特点,设置相关阈值及权重,直观比较了四种插值方法产生的插值结果,选择更适合复杂景区DEM插值的插值方法并对其进行精度提升,以此得到符合精度要求的DEM数据。最后利用相应地区的卫星影像进行地图投影及影像贴图,两种数据结合构建复杂景区的三维地图模型,给游客带来更为精确直观的定位信息和空间要素信息。     

连续台风时期香港区域的水汽变化分析

利用香港卫星定位参考站网GNSS观测数据,提取强热带风暴"塔拉斯"与热带风暴"洛克"影响期间各测站天顶方向对流层延迟,反演香港区域大气可降水量;根据香港区域49个天文台气象站提供的实测降雨量数据,分析大气可降水量与实际降雨量的相关性,以及两次台风对香港区域水汽时空分布的不同影响。结果表明,大气可降水量在台风影响前期均上升,在大量降雨后回落,但在连续台风的间歇期间,仍高于台风来临前的水平;水汽累积是大量降雨的前提条件,当水汽累积量相近时,水汽累积时长与累积降雨量呈正相关;台风期间大气可降水量值超过65 mm的区域面积与台风等级相关,台风路径对局部水汽分布有一定的影响。     

Variations in glacier volume and snow cover and their impact on lake storage in the Paiku Co Basin,in the Central Himalayas

Glaciers and snow cover are important constituents of the surface of the Tibetan Plateau. The responses of these phenomena to global environmental changes are sensitive, rapid and intensive due to the high altitudes and arid cold climate of the Tibetan Plateau. Based on multisource remote sensing data, including Landsat images, MOD10A2 snow product, ICESat, Cryosat-2 altimetry data and long-term ground climate observations, we analysed the dynamic changes of glaciers, snow melting and lake in the Paiku Co basin using extraction methods for glaciers and lake, the degree-day model and the ice and lake volume method. The interaction among the climate, ice-snow and the hydrological elements in Paiku Co is revealed. From 2000 to 2018, the basin tended to be drier, and rainfall decreased at a rate of −3.07 mm/a. The seasonal temperature difference in the basin increased, the maximum temperature increased at a rate of 0.02°C/a and the minimum temperature decreased at a rate of −0.06°C/a, which accelerated the melting from glaciers and snow at rates of 0.55 × 10⁷ m³/a and 0.29 × 10⁷ m³/a, respectively. The rate of contribution to the lake from rainfall, snow and glacier melted water was 55.6, 27.7 and 16.7%, respectively. In the past 18 years, the warmer and drier climate has caused the lake to shrink. The water level of the lake continued to decline at a rate of −0.02 m/a, and the lake water volume decreased by 4.85 × 10⁸ m³ at a rate of −0.27 × 10⁸ m³/a from 2000 to 2018. This evaluation is important for understanding how the snow and ice melting in the central Himalayas affect the regional water cycle.     

栉孔扇贝foxl2基因在卵子发生中的必要性

采用RNAi技术研究了栉孔扇贝(Chlamysfarreri)foxl2基因在卵子发生和卵巢功能维持中的作用。使用体外合成的栉孔扇贝foxl2双链RNA(dsRNA),以每只50μg/次的剂量注射进闭壳肌中,连续注射2次(第一次注射后7天,再进行第二次注射);以注射相同体积PBS(相同注射方法)的扇贝作为阴性对照组,以不注射扇贝作为空白对照组。qRT-PCR检测干扰组扇贝卵巢中的foxl2 mRNA表达量较空白对照组下降了62%, Western blotting检测该蛋白在卵巢中的含量也明显下降,显示靶基因的表达水平被有效地敲降。组织学观察发现,栉孔扇贝foxl2干扰后的卵巢中卵母细胞形态异常、细胞核固缩,卵子发生明显受阻。表明该基因在栉孔扇贝卵子发生中起重要作用。     

Three-dimensional finite element simulation of fracture propagation in rock specimens with pre-existing fissure(s) under compression and their strength analysis

A FORTRAN program, consistent with the commercially available finite element (FE) code ABAQUS, is developed based on a three-dimensional (3D) linear elastic brittle damage constitutive model with two damage criteria. To consider the heterogeneity of rock, the developed FORTRAN program is used to set the stiffness and strength properties of each element of the FE model following a Weibull distribution function. The reliability of the program is assessed against available experimental results for granite cylindrical specimens with a throughgoing, flat and inclined fissure. The calibration procedure of the material parameters is explained in detail, and it is shown that the compressive to tensile strength ratio can have a substantial influence on the failure response of the specimens. Numerical simulations are conducted for models with different levels of heterogeneity. The results show a smaller load bearing capacity for models with less homogeneity, representing gradual coalescence of fully damaged elements forming throughout the models during loading. The maximum load bearing capacity is studied for various combinations of inclination angles of two centrally aligned, throughgoing and flat fissures of equal length embedded in cylindrical models under uniaxial and multiaxial loading conditions. The key role of the compressive to tensile strength ratio is highlighted by repeating certain simulations with a lower compressive to tensile strength ratio. It is proven that the peak loads of the rock models with sufficiently small compressive to tensile strength ratios containing two throughgoing fissures of equal length are similar, provided that the minimum inclination angles of the models are the same. The results are presented and discussed with respect to the existing experimental findings in the literature, suggesting that the numerical model applied in this study can provide useful insight into the failure behaviour of rock-like materials.     

Wetland hydroperiod classification in the western prairies using multitemporal synthetic aperture radar

Wetlands represent one of the world's most biodiverse and threatened ecosystem types and were diminished globally by about two‐thirds in the 20th century. There is continuing decline in wetland quantity and function due to infilling and other human activities. In addition, with climate change, warmer temperatures and changes in precipitation and evapotranspiration are reducing wetland surface and groundwater supplies, further altering wetland hydrology and vegetation. There is a need to automate inventory and monitoring of wetlands, and as a study system, we investigated the Shepard Slough wetlands complex, which includes numerous wetlands in urban, suburban, and agricultural zones in the prairie pothole region of southern Alberta, Canada. Here, wetlands are generally confined to depressions in the undulating terrain, challenging wetlands inventory and monitoring. This study applied threshold and frequency analysis routines for high‐resolution, single‐polarization (HH) RADARSAT‐2, synthetic aperture radar mapping. This enabled a growing season surface water extent hyroperiod‐based wetland classification, which can support water and wetland resource monitoring. This 3‐year study demonstrated synthetic aperture radar‐derived multitemporal open‐water masks provided an effective index of wetland permanence class, with overall accuracies of 89% to 95% compared with optical validation data, and RMSE between 0.2 and 0.7 m between model and field validation data. This allowed for characterizing the distribution and dynamics of 4 marsh wetlands hydroperiod classes, temporary, seasonal, semipermanent, and permanent, and mapping of the sequential vegetation bands that included emergent, obligate wetland, facultative wetland, and upland plant communities. Hydroperiod variation and surface water extent were found to be influenced by short‐term rainfall events in both wet and dry years. Seasonal hydroperiods in wetlands were particularly variable if there was a decrease in the temporary or semipermanent hydroperiod classes. In years with extreme rain events, the temporary wetlands especially increased relative to longer lasting wetlands (84% in 2015 with significant rainfall events, compared with 42% otherwise).