不同盐度对文蛤(Meretrix meretrix)呼吸代谢及体内酶活性的影响

为研究不同盐度对文蛤呼吸代谢的影响,本实验设置5个盐度(‰)梯度(11、18、25、32、39),检测不同盐度对文蛤(Meretrixmeretrix)耗氧和排氨的影响,以及文蛤的外套膜、鳃、肝胰腺三种组织中乳酸脱氢酶和Na+/K+-ATP酶活性的变化。结果表明:随着盐度的不断升高,文蛤耗氧率先升后降再升,在盐度18时达到最大值;排氨率先升后降,在盐度32时达到最大值。随着盐度不断升高和胁迫时间延长,文蛤的肝胰腺中乳酸脱氢酶活力总体呈先升高后下降再升高的趋势(P0.05),酶活力在盐度39时为最高;随着盐度不断升高和胁迫时间延长,文蛤的外套膜中Na+/K+-ATP酶活力总体呈先下降再升高后下降的趋势(P0.05),在盐度32时为最高;文蛤的外套膜和鳃中乳酸脱氢酶活力以及鳃和肝胰腺中Na+/K+-ATP酶活力受盐度影响不显著(P0.05),酶活力变化也多呈现"W"形的变化趋势。研究结果为文蛤的人工养殖提供参考。     

Ephemeral rollover points and clinothem evolution in the modern Po Delta based on repeated bathymetric surveys

Reconstructions of ancient delta systems rely typically on a two-dimensional (2D) view of prograding clinothems but may miss their three-dimensional (3D) stratigraphic complexity which can, instead, be best documented on modern delta systems by integrating high-resolution geophysical data, historical cartography, core data and geomorphological reconstructions offshore. We quantitatively compare three precisely positioned, high-resolution multi-beam bathymetry maps in the delta front and pro delta sectors (0.3 to 10 m water depth) of Po di Pila, the most active of the modern Po Delta five branches. By investigating the detailed morphology of the prograding modern Po Delta, we shed new light on the mechanisms that control the topset to foreset transition in clinothems and show the temporal and spatial complexity of a delta and its pro delta slope, under the impact of oceanographic processes. This study documents the ephemeral nature of the rollover point at the transition between sandy topset (fluvial, delta plain to mouth-bar) and muddy seaward-dipping foreset deposits advancing, in this case, in >20 m of water depth. Three multibeam surveys, acquired between 2013 and 2016, document the complexity in space and time of the topset and foreset regions and their related morphology, a diagnostic feature that could not be appreciated using solely 2D, even very high-resolution, seismic profiles. In addition, the comparison of bathymetric surveys gathered with one-year lapses shows the migration of subaqueous sand dunes on the clinothem topset, the formation of ephemeral cut-and-fill features at the rollover point (few m below mean sea level), the presence of collapse depressions derived by sagging of sediments and fluid expulsion (possibly induced by storm waves) on the foreset, and splays of sand likely reflecting gravity flows on the lower foreset. Though the modern Po Delta is anthropogenic in many respects, its subaqueous clinothem can be studied as a scale model for ancient clinothems that are less resolved geometrically and far less constrained chronologically.     

Variations in glacier volume and snow cover and their impact on lake storage in the Paiku Co Basin,in the Central Himalayas

Glaciers and snow cover are important constituents of the surface of the Tibetan Plateau. The responses of these phenomena to global environmental changes are sensitive, rapid and intensive due to the high altitudes and arid cold climate of the Tibetan Plateau. Based on multisource remote sensing data, including Landsat images, MOD10A2 snow product, ICESat, Cryosat-2 altimetry data and long-term ground climate observations, we analysed the dynamic changes of glaciers, snow melting and lake in the Paiku Co basin using extraction methods for glaciers and lake, the degree-day model and the ice and lake volume method. The interaction among the climate, ice-snow and the hydrological elements in Paiku Co is revealed. From 2000 to 2018, the basin tended to be drier, and rainfall decreased at a rate of −3.07 mm/a. The seasonal temperature difference in the basin increased, the maximum temperature increased at a rate of 0.02°C/a and the minimum temperature decreased at a rate of −0.06°C/a, which accelerated the melting from glaciers and snow at rates of 0.55 × 10⁷ m³/a and 0.29 × 10⁷ m³/a, respectively. The rate of contribution to the lake from rainfall, snow and glacier melted water was 55.6, 27.7 and 16.7%, respectively. In the past 18 years, the warmer and drier climate has caused the lake to shrink. The water level of the lake continued to decline at a rate of −0.02 m/a, and the lake water volume decreased by 4.85 × 10⁸ m³ at a rate of −0.27 × 10⁸ m³/a from 2000 to 2018. This evaluation is important for understanding how the snow and ice melting in the central Himalayas affect the regional water cycle.     

Subsurface flow measurements using passive flux meters in variably-saturated cold-regions landscapes

To date, passive flux meters have predominantly been applied in temperate environments for tracking the movement of contaminants in groundwater. This study applies these instruments to reduce uncertainty in (typically instantaneous) flux measurements made in a low-gradient, wetland dominated, discontinuous permafrost environment. This method supports improved estimation of unsaturated and over-winter subsurface flows which are very difficult to quantify using hydraulic gradient-based approaches. Improved subsurface flow estimates can play a key role in understanding the water budget of this landscape.     

栉孔扇贝foxl2基因在卵子发生中的必要性

采用RNAi技术研究了栉孔扇贝(Chlamysfarreri)foxl2基因在卵子发生和卵巢功能维持中的作用。使用体外合成的栉孔扇贝foxl2双链RNA(dsRNA),以每只50μg/次的剂量注射进闭壳肌中,连续注射2次(第一次注射后7天,再进行第二次注射);以注射相同体积PBS(相同注射方法)的扇贝作为阴性对照组,以不注射扇贝作为空白对照组。qRT-PCR检测干扰组扇贝卵巢中的foxl2 mRNA表达量较空白对照组下降了62%, Western blotting检测该蛋白在卵巢中的含量也明显下降,显示靶基因的表达水平被有效地敲降。组织学观察发现,栉孔扇贝foxl2干扰后的卵巢中卵母细胞形态异常、细胞核固缩,卵子发生明显受阻。表明该基因在栉孔扇贝卵子发生中起重要作用。     

基于高斯两步移动搜寻法的村镇避难场所可达性研究

避难场所可达性是指避难场所与受灾居民之间的通达性,即灾害发生时,避难人员通过避难疏散通道到达避难场所的难易程度,是评价避难场所布局合理性的重要指标。本研究结合高斯两步移动搜寻法和网络分析法,从供需双向出发对村镇地区避难场所进行可达性分析。2种方法的结合,既充分考虑了需求点与设施点的相互作用,又充分考虑了设施点的吸引力随距离的衰减关系,并基于实际道路,以步行疏散时间作为搜索半径,降低了传统研究中因忽略供需间相互作用及实际距离而引起的可达性结果的误差。最后,以神农架松柏镇区为例,验证该方法的实际应用价值。结果表明,该方法能够有效测定村镇地区避难场所的空间可达性,同时,基于GIS软件可以直观地揭示研究区域内避难场所可达性空间分布差异。该方法可为制定科学的村镇防灾减灾规划提供方法支持。     

Properties of geogrid-reinforced marine slope due to the groundwater level changes

Evaluation of slope stability, especially in the absence of a proper bed such as marine soils, is one of the most important issues in geotechnical engineering. Using geogrid layers to enhance the strength and stability of embankments is regarded as a commendable stabilization method. On the other hand, groundwater level erratically fluctuates in coastal areas. Therefore, the aim of this research is to study the effects of groundwater level changes on stability of a geogrid-reinforced slope on loose marine soils in Qeshm Island, Iran. At first, geotechnical properties of the site were obtained by comprehensive series of geotechnical laboratory and in situ tests. Then, by simultaneous changes of groundwater level and several parameters such as embankment slope, loading, geogrid length, geogrid number, and tensile strength of geogrid, different characteristics such as embankment safety factor (SF), vertical and horizontal displacements at embankment top and embankment base were studied. It was observed that groundwater level had significant effects on behavior of the embankment. For most of the observations, by decreasing the groundwater level, the displacements decreased and consequently safety factor increased. Increasing the length, number, and tensile strength of geogrid led to the reduction of displacements and an increase in the safety factor.     

基于2 m格网点云数据的高精度DEM制作第三次国土调查坡度图

地形地貌随着时间的推移时刻发生变化，以高精度数字高程模型（EDE）数据制作坡度图，进而计算田坎系数，更精准地进行耕地面积计算及统计。以2 m格网点云数据生成的DEM数据为基础，按照第三次全国国土调查的相关技术要求，根据耕地坡度分级要求进行分级，生成坡度分级栅格数据图。对坡度分级栅格数据图进行矢量化，生成坡度分级矢量化数据。对矢量化数据进行图斑综合、界线平滑、拓扑重建、数据裁切等处理，制作完成调查区域坡度图及相关属性数据制作。并对生产的坡度图成果进行分析，找出不同尺度格网生产的坡度图的技术差异，并对高精度坡度的应用进行了展望。     

河外H2O超脉泽源及与其相关的分子谱线的观测和研究

自从在活动星系核NGC4945的视线方向上发现第一个河外H2O超脉泽源以来，迄今为止已发现了19个河外H2O超脉泽源，对与活动星系核成协和河外H2O超脉泽源及分子谱线的观测和研究是探测和研究活动星系核核区中央源，拱核气体和尘埃环性质的非常有效的工具，主要评述对河外H2O超脉泽源及与其相关分子谱线的搜索，观测和理论研究现状。