大豆酶解蛋白对凡纳滨对虾幼虾生长性能、血清生化指标、非特异性免疫力和抗病力的影响

【目的】研究大豆酶解蛋白对幼虾生长性能、血清生化指标、非特异性免疫力和抗病力的影响。【方法】凡纳滨对虾(Litopenaeus vannamei)基础饲料添加质量分数0(对照)、1.0%、1.5%、2.0%、2.5%、3.0%、3.5%和4.0%大豆酶解蛋白,配制8种等氮等脂饲料,饲喂凡纳滨对虾幼虾56 d。【结果】1.5%组的增重率和特定生长率最高,显著高于2.0%—4.0%添加组(P0.05);1.0%和1.5%组全虾粗蛋白质含量高于对照组(P0.05),对照、1.0%和1.5%组蛋白质沉积率高于其他各组(P0.05);饲料中添加1.5%~4.0%大豆酶解蛋白可显著提高肌肉磷含量(P0.05),3.0%组最大;1.0%、1.5%和2.0%组血清胆固醇含量低于对照组(P0.05);饲料中添加1.5%~4.0%大豆酶解蛋白可显著提高血清中溶菌酶活性(P0.05),添加1.0%~3.0%大豆酶解蛋白可显著提高血清中酚氧化物酶活性(P0.05),2.5%和3.0%组血清中超氧化物歧化酶活性与1.0%和1.5%组血清中酸性磷酸酶活性高于对照组(P0.05),添加大豆酶解蛋白可显著提高血清中碱性磷酸酶活性(P0.05),2.5%组最大;哈维弧菌(Vibrio harveyi)攻毒7 d后,1.0%组累积死亡率最低。饲料中添加大豆酶解蛋白未提高凡纳滨对虾的生长性能,添加剂量高于3.5%可导致生长性能下降,添加质量分数1.0%大豆酶解蛋白可明显提高凡纳滨对虾的抗病力。【结论】饲料中添加大豆酶解蛋白不能提高凡纳滨对虾的生长性能,高于3.5%添加组的生长性能下降;添加质量分数1.0%大豆酶解蛋白可明显提高凡纳滨对虾的抗病力。     

不同产地浒苔对幼刺参生长、消化和非特异性免疫的影响

为探究不同产地浒苔型饵料对幼刺参生长、消化和非特异性免疫的影响,本实验将青岛浒苔与宁波浒苔的干粉与海泥分别按一定质量比例混合,开展刺参饲喂实验,并与刺参天然饵料马尾藻进行对比。结果表明,青岛浒苔饵料和马尾藻饵料喂养的刺参的粗蛋白含量分别为14.31%±0.10%和15.43%±1.41%,显著高于宁波浒苔饵料(11.17%±0.63%),粗脂肪和灰分含量无显著差异;青岛浒苔组、宁波浒苔组和马尾藻组的增重率分别为22.65%±5.68%、3.03%±1.17%和20.47%±2.01%,特定生长率分别为(1.44±0.33)、(0.21±0.08)、(1.33±0.12)%/d,青岛浒苔组和马尾藻组刺参的增重率和特定生长率显著高于宁波浒苔组;青岛浒苔组、宁波浒苔组和马尾藻组刺参肠道淀粉酶、胃蛋白酶、胰蛋白酶和纤维素酶活力无显著差异;马尾藻组刺参体腔液碱性磷酸酶活力为(17.57±4.56)金氏单位/100mL,显著高于青岛浒苔组[(5.56±1.32)金氏单位/100mL]和宁波浒苔组[(2.83±0.75)金氏单位/100mL],超氧化物歧化酶和酸性磷酸酶活力无显著差异。由此可见,绿潮暴发时,通过打捞浒苔用以配制刺参饵料,既有助于缓解绿潮的生态灾害,又能够补充刺参饵料来源,具有广阔的生态效益和市场前景,但是其营养成分影响因素较为复杂,配制饵料时应充分考虑不同品种、采集时间和生长地点的差异,并通过一些前处理手段充分发挥浒苔的饵料价值。     

玉米素和水杨酸对雨生红球藻(Haematococcus pluvialis)生长及虾青素积累的影响

虾青素是一种具有强抗氧化活性的类胡萝卜素,而雨生红球藻是天然虾青素的主要来源。本文以雨生红球藻(Haematococcus pluvialis)为材料,研究了植物激素玉米素和水杨酸对雨生红球藻的生长、虾青素含量及相关基因表达的影响。分别添加5种浓度的玉米素或水杨酸,结果发现0.05mg/L玉米素或25mg/L水杨酸处理5d后雨生红球藻虾青素积累最多。该浓度玉米素或水杨酸可显著提高光胁迫下藻细胞密度,最高分别达到3.4×10~5cell/mL和3.0×10~5cell/mL;同时玉米素与水杨酸组中虾青素含量显著上升,分别为1.7%和1.6%,比对照组分别增加29.2%和25.6%。玉米素缓解了高光逆境条件下光合作用基因——Rubisco大亚基(rbcL)及其活化酶(rca)、碳酸酐酶(ca)的下调表达,但对虾青素合成途径β-胡萝卜素酮化酶基因(bkt)的表达量没有显著影响;而水杨酸则相反,在胁迫后期不能缓解光合作用相关基因的下调表达,但可使bkt基因显著上调,最高可达对照组的2.5倍。本研究首次比较了玉米素和水杨酸对雨生红球藻生长和虾青素积累的影响,发现玉米素比水杨酸具有更好的促进雨生红球藻中虾青素积累的效果。     

一种新型浅地层剖面仪试验平台的设计与应用

为探讨浅地层剖面声学影像形成原理,探索不同类型沉积物声学影像特征,厘清不同沉积物厚度的识别算法,采用模块化设计理念研发一套新型浅地层剖面仪室内试验平台。综合室内定位技术、浅剖试验平台尺寸合理性分析技术、直线轨道与换能器之间的固定连接技术和海底声学参数反演技术,形成一个试验高效的新型平台。通过铺设沉积物和布设障碍物,利用浅地层剖面仪进行走航测试,精确识别了沉积物的厚度和障碍物的位置。该平台可为开展理想环境下沉积物厚度和障碍物识别提供较好的测试环境,为声学海洋设备性能检测提供测试平台,也为高校学生和技术人员提供设备使用培训场所。     

台湾地层检讨(续)

所谓‘大寮层’者,以页岩与砂岩之交互层为主,厚约350公尺,在其上部,砂岩发育较佳,而在山下部则反是。砂岩往住带石灰质并含有化石,尤其在上部有富含Ditrupa sp之灰质的砂岩发现,所谓Ditrupa化石带是也。此外尚有下列之化石发现:     

The Geologic Time Scale and the Italian stratigraphic record

The construction of the Geologic Time Scale (GTS) is a titanic scientific challenge that has been under way for two centuries and will require much dedicated effort in the future. Italy preserves a paramount stratigraphic record of Mesozoic and Cenozoic marine sediments that have been significant in the development of the modern GTS. The Italian stratigraphic record has been histori-cally important in introducing and defining the standard Chronostratigraphic Units (CUs) of the Neogene and Quaternary. Pelagic successions from Northern Apen-nines and Southern Alps have been used in the seventies for integrating the late Cretaceous-Paleogene Geomag-netic Polarity Time Scale (GPTS) with planktonic microfossil biostratieraohv and standard CUs. This was a major contribution to the construction of a new gener-ation of GTS based on integrated magnetobiochronol-ogy. The middle Miocene to early Pleistocene marinerecord from Sicily and southern Italy has been funda-mental for establishing the recently developed Astro-nomical Time Scale (ATS). In prospect, there are many potentials still to be exploited in the Italian marine stratigraphic record for implementing the GTS by defin-ing GSSPs of various CUs, improving magneto-biochronology and extending downwards the ATS.     

蜕壳素对凡纳滨对虾生长及表观消化率的影响

研究了外源性蜕皮激素对凡纳滨对虾生长和表观消化率的影响 ,实验时在基础饲料中分别添加 0 .1%、0 .2 %、0 .4 %的两种市售蜕壳素产品 ,制成 6种外源性脱壳素配合饲料 ,不添加的基础饲料作为对照组 ,试验在 2 6 0L玻璃钢循环水养殖系统中 ,每个剂量组设 3个重复 ,8周生长试验结果表明 :市售蜕壳素产品能有效地促进对虾生长 ,试验结束时 ,试验组虾体增重分别较对照组增加 16 .8%、7.5 2 %、5 .9%、11.4 %、2 9.0 7%、16 .5 3% ,但是凡纳滨对虾对饲料粗蛋白的表观消化率没有明显地提高 ,凡纳滨对虾对脂肪的表观消化率较低。推算凡纳滨对虾饲料中最佳蜕皮酮添加量为 6 0mg/kg。     

STUDY ON THE INTERACTION BETWEEN NDVI PROFILE AND THE GROWING STATUS OF CROPS

Daily and ten-day Normalized Difference Vegetation Index(NDVI) of crops were retrieved from meteorological statellite NOAA AVHRR images ,The temporal variations of the NDVI were analyzed during the whole growing season,and thus the principle of the interaction between NDIV profile and the growing status of crops was discussed,As a case in point,the relationship between integral NDVI and winter wheat yield of Henan Province in 1999 had been analyzed.By putting integral NDVI values of 60 sample counties into the winter wheat yield-integral NDVI coordination,scattering map was plotted. It demonstrated that integral NDVI had a close relation with winter wheat yield.These relation could be described with linear,cubic polynomial ,and exponential regression,and the cubic polynomial regression was the best way,In general ,NDVI reflects growing status of green vegetation ,so crop monitoring and crop yield estimation could be realized by using remote sensing technique on the basis of time serial NDVI data together with agriculture calendars.