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1.
运用电脉冲转基因仪对合浦珠母贝卵子进行电脉冲处理以优化电穿孔法转基因的电击参数。实验中调制百分比:100%;脉冲间隔:1.0s:电极距离:2mm;脉冲个数:5个,参数保持不变。用不同的电脉冲参数组合(脉冲电压100-400V、脉冲持续时间0.5~3.0ms、脉冲频率10~50kHz,共28个组合)对卵子电击,然后加入精子进行授精,统计各组受精率和孵化率,以受精率和孵化率达到50%以上为衡量指标。结果表明,合浦珠母贝卵子对低脉冲频率和高电压非常敏感,当频率低于20kHz或脉冲电压高于400V时对卵子损伤较大。脉冲持续时间对其影响不大,适应范围较宽。优化的转基因电击脉冲参教组合为:脉冲电压1.0kV/cm,脉冲持续时间20ms,脉冲频率50kHz,或者脉冲电压1.5kV/cm,脉冲持续时间1.5ms,脉冲频率50kHz。 相似文献
2.
研究水的声吸收所引起的光声脉冲幅度的变化。脉冲声波由激光通过光击穿机制产生。文中给出了光声脉冲峰值声压与传播距离和声吸收系数之间的理论关系式。实验测量结果和理论曲线所描述的变化规律相符合。 相似文献
3.
高空间分辨率遥感影像中地物目标内部光谱信息复杂性的增强,使得传统基于光谱特征值的数据处理方法效果不再显著,影像分割为解决这一问题提供了一种思路,成为当前高空间分辨率遥感影像处理的研究焦点.时刻独立脉冲耦合神经网络具有状态相近、空间相邻神经元相互耦合同步脉冲激发和区域之间神经元脉冲激发时刻独立两大特点,已被应用于非遥感影像分割中,并取得较好效果.本文结合高空间分辨率遥感影像特点,通过对网络参数进行实验和分析,提出一个基于时刻独立脉冲耦合神经网络的高空间分辨率遥感影像分割方法,并利用空间分辨率0.3m的航空影像进行了数据试验,将分割结果进行讨论并与现有时刻独立脉冲耦合神经网络方法和ISODATA方法分割结果进行对比分析.结果表明:时刻独立脉冲耦合神经网络在高空间分辨率遥感影像分割处理中具有很好的应用前景. 相似文献
4.
We have reinvestigated the mid-Cretaceous plume pulse in relation to paleo-oceanic plateaus from accretionary prisms in the circum-Pacific region, and we have correlated the Pacific superplume activity with catastrophic environmental changes since the Neoproterozoic. The Paleo-oceanic plateaus are dated at 75–150 Ma; they were generated in the Pacific superplume region and are preserved in accretionary prisms. The volcanic edifice composed of both modern and paleo-oceanic plateaus is up to 10.7 × 106 km2 in area and 19.1 × 107 km3 in volume. The degassing rate of CO2 (0.82 − 1.1 × 1018 mol/m.y.) suggests a significant impact on Cretaceous global warming. The synchronous occurrence of paleo-oceanic plateaus in accretionary complexes indicates that Pacific superplume pulse activities roughly coincided at the Permo-Triassic boundary and the Vendian–Cambrian boundary interval. The CO2 expelled by the Pacific superplume probably contributed to environmental catastrophes. The initiation of the Pacific superplume contributed to the snowball Earth event near the Vendian–Cambrian boundary; this was one of the most dramatic events in Earth's history. The scale of the Pacific superplume activity roughly corresponds to the scale of drastic environmental change. 相似文献
5.
6.
本文通过对雷电放电分析,研究解决微震仪有线传输的避雷问题,并介绍接收雷电信号控制传输线上电荷对地短路的“DBL-1”避雷仪。 相似文献
7.
本文用长周期763地震仪面波群速度资料反演了中国南北带及邻区的三维速度结构.其中采集238条瑞利波和358条勒夫波混合频散曲线,使用均等显示滤波方法,并以4°×4°为一格将我国境内分为147格.用随机逆反演方法得到了研究区16格的纯路径频散.面波速度结构及演结果表明:1.莫霍界面深度一般在40-50km之间,最深达65km.总趋势是从东到西加深,且在南北带西侧南北两端向中部明显加深,东侧变化小.2.地幔顶部普遍出现很厚的低速层,上界面一般埋深60-80km.上地幔顶盖厚度一般为20-60km,速度为4.30-4.50km/s.3.研究区普遍存在各向异性,而且勒夫波和瑞利波速度的差值(VSV-VSH)的绝对值随深度有增大的特点,在南北带南部和西北部VSV-VSH各向异性现象更为明显. 相似文献
8.
Strength and stability of frictional sliding of gabbro gouge at elevated temperatures 总被引:7,自引:1,他引:7
To investigate the strength of frictional sliding and stability of mafic lower crust, we conducted experiments on oven-dried gabbro gouge of 1 mm thick sandwiched between country rock pieces (with gouge inclined 35° to the sample axis) at slip rates of 1.22 × 10− 3 mm/s and 1.22 × 10− 4 mm/s and elevated temperatures up to 615 °C. Special attention has been paid to whether transition from velocity weakening to velocity strengthening occurs due to the elevation of temperature.Two series of experiments were conducted with normal stresses of 200 MPa and 300 MPa, respectively. For both normal stresses, the friction strengths are comparable at least up to 510 °C, with no significant weakening effect of increasing temperature. Comparison of our results with Byerlee's rule on a strike slip fault with a specific temperature profile in the Zhangbei region of North China shows that the strength given by experiments are around that given by Byerlee's rule and a little greater in the high temperature range.At 200 MPa normal stress, the steady-state rate dependence a − b shows only positive values, probably still in the “run-in” process where velocity strengthening is a common feature. With a normal stress of 300 MPa, the values of steady-state rate dependence decreases systematically with increasing temperature, and stick-slip occurred at 615 °C. Considering the limited displacement, limited normal stress applied and the effect of normal stress for the temperatures above 420 °C, it is inferred here that velocity weakening may be the typical behaviour at higher normal stress for temperature above 420 °C and at least up to 615 °C, which covers most of the temperature range in the lower crust of geologically stable continental interior. For a dry mafic lower crust in cool continental interiors where frictional sliding prevails over plastic flow, unstable slip nucleation may occur to generate earthquakes. 相似文献
9.
How to obtain alert velocity thresholds for large rockslides 总被引:1,自引:0,他引:1
A reliable forecast of the failure stage of large rockslides is difficult, because of non-linear time dependency of displacements and seasonal effects. Aim of this paper is to suggest a practical method to prepare alert thresholds for large rockslides, assessing critical values of velocity for carrying out civil protection actions using monitoring data. Adopted data concern the 20 Mm3 Ruinon rockslide (Valfurva, Central Alps, Italy), still evolving and suitable to originate a fast moving rock avalanche. Multitemporal analysis of aerial photos, LIDAR-ALTM laser topography, field survey and geomechanical analyses allowed to infer the rockslide kinematics and better understand data provided by a monitoring network including distometers, extensometers, GPS benchmarks and inclinometers. The analysis of displacement and rainfall data over five years (1997–2001) allowed to recognise three different evolutionary patterns of displacements, showing a continuously increasing rate since 1997. Data representing large-scale behaviour of the rock mass were fitted by power-law curves, according to the “accelerating creep” model by Voight, in order to evaluate a suitable failure time. This was hampered by the large seasonal deviations, which can significantly delay the occurrence of failure. Data were fitted using the Voight’s equation, expressed in terms of displacement, through non-linear estimation techniques, in order to find values of the controlling parameters (A, α and tf) suitable to represent the mechanical behaviour of the rock mass approaching the failure. This allowed to compute velocity–time theoretical curves and to define different velocity threshold values (pre-alert, alert and emergency) to be used for emergency management. 相似文献
10.