Enhanced a novel β-agarase production in recombinant Escherichia coli BL21(DE3) through induction mode optimization and glycerol feeding strategy

Agarases are hydrolytic enzymes that act on the hydrolysis of agar and have a broad range of applications in food,cosmetics and pharmaceutical industries. In this study, a glycerol feeding strategy based on induction mode optimization for high cell density and β-agarase production was established, which could effectively control acetate yield. First, exponential feeding strategy of glycerol with different overall specific growth rates(μ) was applied in the pre-induction phase. The results showed that the low μ(μ=0.2) was suggested to be the optimal for cell growth and β-agarase production. Second, the effects of induction temperature and the inducer concentration on cell growth and β-agarase production were investigated in the post-induction phase. When induced by isopropyl-β-d-thiogalactoside(IPTG), the strategy of 0.8 mmol/L IPTG induction at 20℃ was found to be optimal for β-agarase production. When cultivation was induced by continuous lactose feeding strategy of 1.0 g/(L·h), the β-agarase activity reached 112.5 U/mL, which represented the highest β-agarase production to date.Furthermore, the β-agarase was capable of degrading G. lemaneiformis powder directly to produce neoagarooligosaccharide, and the hydrolysates were neoagarotetraose(NA4) and neoagarohexaose(NA6). The overall research may be useful for the industrial production and application of β-agarase.     

城区水文循环机制与改善策略分析

在城区化过程中,由于人口快速成长且集中,随之而来的建筑物、道路等不透水面密度增加,导致地下水补给减少及破坏水文循环。为了解土地利用方式改变对长期水文量影响,建立一个适宜的城区水循环模式是非常重要的。选定台湾台北的六馆抽水站集水区进行分析,模式参数经过敏感度分析、率定与验证。采用改变不同透水铺面设置比例的方案,评估其对径流量、入渗量、蒸发散量的改变。并分析设置不同比例屋顶雨水贮集系统对地表径流量及供水量的改变。所建立的城区水循环模式是评估土地利用改变造成水文量的改变的一个有效工具,并可提供决策者做为城区土地利用决策的参考。     

中国中西部地区地震的重新定位和三维地壳速度结构

采用中国中西部地区(21~36N， 98~112E)193个地震台在1992～1999年间记录到的9 988次地震的Pg和Sg震相走时的读数资料，用Roecker的SPHYPIT90程序，反演了该地区三维地壳P波速度结构，并用SPHREL3D90程序进行了地震的重新定位．反演结果揭示了中国中西部地区地震P波速度结构明显的横向不均匀性，这些不同深度上波速的横向变化多以该地区的活动断裂为分界线．可以看出活动断裂两侧存在明显的速度反差．通过重新定位，得到了6 459次地震的震源参数，这些精确定位的地震震中明显沿该区活动断裂呈现条带状分布，其范围和尺度清晰地表示了这一地区地震活动与活动断裂的紧密关系．其中，82％重新精确定位的事件的震源深度在20 km以内. 这一结果与笔者用双差地震定位法得到的重新定位的震源深度分布相一致．      

Earthquake relocation and 3-dimensional crustal structure of P-wave velocity in cen-tral-western China

IntroductionUnderstandingthemechanismofcontinentalearthquakesisveryimportantforseismichaz-ardpreventionandearthquakeprediction.Themodernseismotectonictheoryandtheideaofearthquakepredictionaredevelopedmainlyfromthestudiesoninterplateearthquakes,whicharedifficulttoexplainthephenomenaofintraplateearthquakes,suchasthecontinentalearthquakesoccurredinChinesemainland.Whiletheinterplateearthquakesoccurredalongtheplatebounda-ries,theintraplateearthquakesdistributediffuselyintheinterioroftheplates.Thus…     

Vertical structures of PM10 and PM 2.5 and their dynamical character in low atmosphere in Beijing urban areas

The vertical structures and their dynamical character of PM2.5 and PM10 over Beijing urban areas are revealed using the 1 min mean continuous mass concentration data of PM2.5 and PM10 at 8, 100, and 320 m heights of the meteorological observation tower of 325 m at Institute of Atmospheric Physics, Chinese Academy of Sciences (IAP CAS tower hereafter) on 10―26 August, 2003, as well as the daily mean mass concentration data of PM2.5 and PM10 and the continuous data of CO and NO2 at 8, 100 (low layer), 200 (middle layer), and 320 m (high layer) heights, in combination with the same period meteorological field observation data of the meteorological tower. The vertical distributions of aerosols observed on IAP CAS tower in Beijing can be roughly divided into two patterns: gradually and rapidly decreasing patterns, I.e. The vertical distribution of aerosols in calm weather or on pollution day belongs to the gradually decreasing pattern, while one on clean day or weak cold air day belongs to the rapidly decreasing pattern. The vertical distributive characters of aerosols were closely related with the dynamical/thermal structure and turbulence character of the atmosphere boundary layer. On the clean day, the low layer PM2.5 and PM10 concentrations were close to those at 8 m height, while the concentrations rapidly decreased at the high layer, and their values were only one half of those at 8 m, especially, the concentration of PM2.5 dropped even more. On the clean day, there existed stronger turbulence below 150 m, aerosols were well mixed, but blocked by the more stronger inversion layer aloft, and meanwhile, at various heights, especially in the high layer, the horizontal wind speed was larger, resulting in the rapid decrease of aerosol concentration, I.e. Resulting in the obvious vertical difference of aerosol concentrations between the low and high layers. On the pollution day, the concentrations of PM2.5 and PM10 at the low, middle, and high layers dropped successively by, on average, about 10% for each layer in comparison with those at 8 m height. On pollution days, in company with the low wind speed, there existed two shallow inversion layers in the boundary layer, but aerosols might be, to some extent, mixed below the inversion layer, therefore, on the pollution day the concentrations of PM2.5 and PM10 dropped with height slowly; and the observational results also show that the concentrations at 320 m height were obviously high under SW and SE winds, but at other heights, the concentrations were not correlated with wind directions. The computational results of footprint analysis suggest that this was due to the fact that the 320 m height was impacted by the pollutants transfer of southerly flow from the southern peripheral heavier polluted areas, such as Baoding, and Shijiazhuang of Hebei Province, Tianjin, and Shandong Province, etc., while the low layer was only affected by Beijing's local pollution source. The computational results of power spectra and periods preliminarily reveal that under the condition of calm weather, the periods of PM10 concentration at various heights of the tower were on the order of minutes, while in cases of larger wind speed, the concentrations of PM2.5 and PM10 at 320 m height not only had the short periods of minute-order, but also the longer periods of hour order. Consistent with the conclusion previously drawn by Ding et al., that air pollutants at different heights and at different sites in Beijing had the character of "in-phase" variation, was also observed for the diurnal variation and mean diurnal variation of PM2.5 and PM10 at various heights of the tower in this experiment, again confirming the "in-phase" temporal/spatial distributive character of air pollutants in the urban canopy of Beijing. The gentle double-peak character of the mean diurnal variation of PM2.5 and PM10 was closely related with the evident/similar diurnal variation of turbulent momentum fluxes, sensible heat fluxes, and turbulent kinetic energy at various heights in the urban canopy. Besides, under the condition of calm weather, the concentration of PM2.5 and PM10 declined with height slowly, it was 90% of 8 m concentration at the low layer, a little lesser than 90% at the middle layer, and 80% at the high layer, respectively. Under the condition of weak cold air weather, the concentration remarkably dropped with height, it was 70% of 8 m concentration at the low layer, and 20%―30% at the middle and high layers, especially the concentration of PM2.5 was even lower.     

Assessing the vegetation canopy influences on wind flow using wind tunnel experiments with artificial plants

Wind erosion causes serious problems and considerable threat in most regions of the world. Vegetation on the ground has an important role in controlling wind erosion by covering soil surface and absorbing wind momentum. A set of wind tunnel experiments was performed to quantitatively examine the effect of canopy structure on wind movement. Artificial plastic vegetations with different porosity and canopy shape were introduced as the model canopy. Normalized roughness length (Z ₀/H) and shear velocity ratio (R) were analyzed as a function of roughness density (λ). Experiments showed that Z ₀/H increases and R decreases as λ reaches a maximum value, λ _max, while the values of Z ₀/H and R showed little change with λ value beyond as λ _max.     

香港世界地质公园晚侏罗世荔枝庄组岩石组合及其成岩地质过程解析

中国东南大陆晚侏罗世地层普遍缺失,仅零星见于个别地区,香港新界东北的荔枝庄组即为其一。荔枝庄组出露于香港世界地质公园沉积岩园区的荔枝庄地区,自下往上由火山岩—沉积岩—火山岩组合而成,沉积岩中发育大型包卷层理和滑塌构造等典型沉积构造,是香港地区最具代表性的晚侏罗世火山—沉积岩系。通过实测地层剖面研究,确定其成岩过程大体上可划分为早期普林尼式火山爆发、中期破火山口湖相沉积和晚期普林尼式火山爆发三个阶段,以湖相沉积作用为主、火山喷发作用为辅;受晚期火山岩浆活动的影响,沉积岩层普遍发生硅化或炭化。荔枝庄组独特的岩石组合与形成的古地理环境,为探讨中国东南大陆中生代火山活动—沉积作用方式与成岩过程,提供了难得的研究实例。     

朝鲜半岛古元古代摩天岭群的碎屑锆石U-Pb年龄及其地质意义

克拉通内部活动带变质沉积建造蕴藏了早期超大陆形成中地体聚-散动力学过程的重要信息,碎屑锆石年代学则是揭示这种信息的最佳工具之一。针对华北(中-朝)克拉通最具代表性的古元古代活动带(辽-吉-朝活动带)中巨量沉积建造精细表征方面的空白区域,本文开展了朝鲜半岛检德地区摩天岭群的碎屑锆石年代学研究。SIMS U-Pb定年结果显示,北大川组变火山碎屑岩样品的锆石年龄谱记录了约2.53Ga的主要年龄峰值和约2.19Ga的最年轻峰值,说明北大川组开始沉积的时代应该晚于2.2Ga。南大川组变沉积岩样品碎屑锆石记录的最年轻峰值年龄为约2.02Ga,说明南大川组的沉积时代应该不早于2.02Ga;其锆石年龄谱记录了一个主要年龄峰值区域(2.02~2.18Ga)、一个次要年龄峰值(约2.46Ga)和一些2.78~3.30 Ga的古老年龄,指示其物质源区主要为同区古元古代地质体和邻近地体上的太古宙变质基底。区域对比表明,摩天岭群与中国境内辽吉活动带中的辽河群及集安群/老岭群具有基本一致的碎屑锆石年龄谱,说明它们不仅是同一时代的产物,而且分享类似的物质源区。这些时代一致、物源相近的沉积建造可以为甄别古元古代辽-吉-朝活动带的大地构造属性提供重要参考。与沉积年龄相近的碎屑锆石占据主导的碎屑锆石年龄谱反映了汇聚板块边缘各类盆地的典型特征,而相悖于大部分碎屑锆石年龄远大于地层沉积年龄的裂谷型盆地。     

朝鲜~19亿年侵入岩的岩石类型与构造背景初探

朝鲜半岛北部广泛发育~19亿年侵入岩,这些岩浆岩可以分为三个系列,分别为似斑状花岗岩系列(多为I型,以妙香山岩体为代表,年龄1900~1840Ma)、S型花岗岩系列(以嘉山岩体为代表,本文获得1862±5Ma锆石U-Pb年龄)和正长岩系列(以龙浦岩体为代表,本文获得1857±2Ma锆石U-Pb年龄)。似斑状花岗岩系列(I型花岗岩)大致对应朝鲜地质学家定义的Myohyangsan(妙香山)杂岩,以发育钾长石巨斑为特征,与麻粒岩相变质的火山岩-沉积岩系关系密切;S型花岗岩系列大致对应Ryonhwasan(莲花山)杂岩,以发育石榴石和堇青石等矿物为特征,与麻粒岩相变质的副变质岩共生:这说明前者可能与变质火山岩系相关,而后者可能和副变质岩相关。正长岩系列,朝鲜地质学家称为Sakju(朔州)杂岩,分布较为局限,仅见于朔州以及博川-定州之间,也见于邻区辽东,多为岩株。这三个系列岩浆岩稍早或者同期于角闪岩相-麻粒岩相变质(本文获得甑山"群"变质锆石U-Pb年龄1844±2Ma)。邻区辽东-吉南及胶东地区也发育这三个系列岩浆岩,但这两个地区~21亿年岩浆作用更为广泛。朝鲜半岛南部发育大量同期岩浆岩,但岩石类型以似斑状花岗岩为主,并发育紫苏花岗岩和斜长岩。我们推测,朝鲜半岛南北两侧基底属性存在差异,可能对应不同的古陆(华北与华南古陆)。综合分析表明,朝鲜~19亿年前广泛发育I型和S型花岗岩,并有幔源岩浆作用,同时发育正长岩类,并且这些岩浆活动与区域高级变质作用时代接近。考虑到本区存在太古宙基底,我们推测本区在古元古代可能处于类似现今活动大陆边缘弧背景。