Induction of vitellogenin in vivo and in vitro in the model teleost medaka (Oryzias latipes): comparison of gene expression and protein levels

Quantification of the egg yolk precursor vitellogenin (VTG) in fish has become a standard technique to detect estrogenic effects of known chemicals and environmental samples. In the present study, we have analysed VTG induction by estradiol, ethynylestradiol and genistein exposure in the model teleost medaka (Oryzias latipes) and demonstrate that the medaka is a suitable model system to analyse estrogenic effects. By comparing VTG gene expression and protein levels we show that in principal both techniques can be used to study VTG induction in vivo (juvenile and adult males) and in vitro (primary cultures of male liver cells). If a short term in vivo or in vitro exposure is performed, detection of mRNA might be sufficient. For long term studies with the need to detect weak estrogenic chemicals and a precise quantification, immuno-chemical detection may be favoured.     

A simple mass estimate for central black holes in cuspy galaxies

We argue that the temperature of the stellar phase is likely a monotically decreasing function of radius in the central regions of elliptical galaxies with cusps. We show that it requires the presence of a central mass concentration. An estimate of its minimum mass based on few parameters is given. This lower bound is consistent with current mass values of the central objects in galaxies derived from detailed models. This revised version was published online in July 2006 with corrections to the Cover Date.     

Analysis of uncertainties in the hydrological response of a model‐based climate change impact assessment in a subcatchment of the Spree River,Germany

Climate change impact assessments form the basis for the development of suitable climate change adaptation strategies. For this purpose, ensembles consisting of stepwise coupled models are generally used [emission scenario → global circulation model → downscaling approach (DA) → bias correction → impact model (hydrological model)], in which every item is affected by considerable uncertainty. The aim of the current study is (1) to analyse the uncertainty related to the choice of the DA as well as the hydrological model and its parameterization and (2) to evaluate the vulnerability of the studied catchment, a subcatchment of the highly anthropogenically impacted Spree River catchment, to hydrological change. Four different DAs are used to drive four different model configurations of two conceptually different hydrological models (Water Balance Simulation Model developed at ETH Zürich and HBV‐light). In total, 452 simulations are carried out. The results show that all simulations compute an increase in air temperature and potential evapotranspiration. For precipitation, runoff and actual evapotranspiration, opposing trends are computed depending on the DA used to drive the hydrological models. Overall, the largest source of uncertainty can be attributed to the choice of the DA, especially regarding whether it is statistical or dynamical. The choice of the hydrological model and its parameterization is of less importance when long‐term mean annual changes are compared. The large bandwidth at the end of the modelling chain may exacerbate the formulation of suitable climate change adaption strategies on the regional scale. Copyright © 2013 John Wiley & Sons, Ltd.     

Radiative transfer in disc galaxies – I. A comparison of four methods to solve the transfer equation in plane-parallel geometry

Accurate photometric and kinematic modelling of disc galaxies requires the inclusion of radiative transfer models. Because of the complexity of the radiative transfer equation (RTE), sophisticated techniques are required. Various techniques have been employed for the attenuation in disc galaxies, but a quantitative comparison of them is difficult, because of the differing assumptions, approximations and accuracy requirements that are adopted in the literature. In this paper, we present an unbiased comparison of four methods to solve the RTE, in terms of accuracy, efficiency and flexibility. We apply them all to one problem that can serve as a first approximation of large portions of disc galaxies: a one-dimensional plane-parallel geometry, with both absorption and multiple scattering taken into account, with arbitrary vertical distributions of stars and dust and an arbitrary angular redistribution of the scattering. We find that the spherical harmonics method is by far the most efficient way to solve the RTE, whereas both Monte Carlo simulations and the iteration method, which are straightforward to extend to more complex geometries, have a cost that is about 170 times larger.     

Exact solution for energy analysis of Schwarzschild black-hole fed by CMBR

The exact closed-form mathematical solution for Schwarzschild black-hole fed by the Cosmic Micro-wave Background Radiance (CMBR) is obtained analytically. This unique solution holds for both growth (evolution) and decay, which are bifurcated by the critical initial mass, which depends on the temperature of CMBR. Results are presented to illustrate this solution in terms of decay/growth of black-hole, and the variation of rate of growth/decay with CMBR.