The effects of electrochemical oxidation on in-vivo fluorescence and toxin content in Microcystis aeruginosa culture

The increasing occurrence of cyanobacterial blooms in water bodies is a serious threat to the environment. Efficient in-lake treatment methods for the control of cyanobacteria proliferation are needed, their in-vivo detection to obtain a real-time response to their presence, as well as the information about their physiological state after the applied treatment. In-vivo fluorescence measurements of photosynthetic pigments have proved to be effective for quantitative and qualitative detection of phytoplankton in a water environment. In the experiment, chlorophyll and phycocyanin fluorescence sensors were used concurrently to detect stress caused by electrochemical oxidation applying an electrolytic cell equipped with borondoped diamond electrodes on a laboratory culture of cyanobacteria Microcystis aeruginosa PCC 7806. The inflicted injuries were reflected in a clear transient increase in the phycocyanin fluorescence signal(for 104 %? 43%) 24 h after the treatment, which was not the case for the chlorophyll fluorescence signal. In the next 72 h of observation, the fluorescence signals decreased(on 40% of the starting signal) indicating a reduction of cell number, which was confirmed by cell count(24% reduction of the starting concentration) and analysis of extracted chlorophyll and phycocyanin pigment. These results demonstrate the viability of the combined application of two sensors as a useful tool for in-vivo detection of induced stress, providing real-time information needed for the evaluation of the efficiency of the in-lake treatment and decision upon the necessity of its repetition. The electrochemical treatment also resulted in a lower free microcystins concentration compared to control.     

Design of Buoys for Mounting Wind Turbines at Exposed Sites

In this study, two designs for a buoy capable of supporting a 10 kW wind turbine and its tower were developed to operate at the University of New Hampshire’s Center of Ocean Renewable Energy testing site located off the Isles of Shoals, New Hampshire. The buoys are to be moored by a catenary chain system. To evaluate wave response, two Froude-scaled models were constructed, tested, and compared at the Ocean Engineering wave tank at the University of New Hampshire. These buoys have been implemented and compared with wave tank measurements of the spar displacement at a reference elevation 2.44 m above the mean water level.     

Foreword

Ocean circulation in the Yellow and East China Seas is very rich in mesoscale phenomena, such as eddies, fronts, upwellings, river plumes, etc. Among others, having drawn tremendous attention from oceanographic community in the past for nearly 50 years are mainly the Yellow Sea Cold Water Mass (YSCWM) and its related circulation, the Yellow Sea Warm Current (YSWC), the East China Sea     

Influence of the Choice of Core-Envelope Transition Point on the Binary Merger of Two Main-sequence Components

We have studied the influence of different choices of core-envelope transition point on the final merger of contact binaries with two main-sequence components. A binary of 1.00 + 0.90M⊙ with an initial orbital period of 0.35d is examined. The mass fraction of the primary mixed with the matter of the secondary, qmix, determined by the chosen core-envelope transition point, ranges from 0.04 to 1.00 in our analysis. If as qmix< 0.8, none of the     

Geochemical survey of thermal system in Northeast China and their geothermometric characters

The chemical composition of several thermal springs around Changbaishan area has been investigated. Cenozoic basaltic rocks are widely distributed in Northeast China and geothermal characteristics have been described. About one hundred hot springs exist around Changhaishan Volcano at the border between China and D.P.R.Korea with high temperature about 82~C. The pH values of the spring water range from 6.9 to 7.1 and the total flow rate is about 4.8 L/sec. The chemical composition of the thermal springs is sodium carbonate; the high-mineral contents of thermal water are believed to have medicinal properties. Bathhouses are already built along the hot springs to take the advantage of the supposed healing properties. The high quality of those hot springs is believed to be utilitized for mineral water. The chemical equilibrium temperatures were estimated at about 160℃ based on the Na-K-Ca geochemical thermometer.     

严厉打击违法行为促进依法合理用地——省国土资源厅公开曝光11起土地违法案件

今年6月25日是<土地管理法>颁布二十周年纪念日,也是第16个"全国土地日".今年土地日的宣传主题是"依法合理用地,促进科学发展".为了加大土地违法案件查处力度,严厉打击土地违法行为,促进依法合理用地,6月22日上午,浙江省国土资源厅在杭州举行新闻发布会,公开曝光11起土地违法案件.新华社浙江分社、人民日报、中央人民广播电台、农民日报、经济日报浙江记者站,浙江日报、浙江卫视、浙江人民广播电台等17家新闻单位参加了新闻发布会.     

Size-Flux Relation in Solar Active Regions

We present a study of the relationship between integral area and corresponding total magnetic flux for solar active regions. It is shown that some of these relationships are satisfied to simple power laws. Fractal examination showed that some of these power laws can not be justified inside the simple models of stationary magnetic flux tube aggregation. All magnetic fluxes and corresponding areas were calculated using the data measured with the Solar Magnetic Field Telescope of the Huairou Solar Observing Station in Beijing.     

Isolation of Protoplasts from Undaria pinnatifida by Alginate Lyase Digestion

1　Introduction　Protoplastsserveasabasicandversatiletoolforge neticengineeringandbiochemicalresearchforseveralreasons :theymayregeneratetheirwallsandprovideamodelsystemforstudyingwallbiogenesis ,theirlysisprovidesagentlemeansofpreparingsubcellularor ganelles ,andthemembraneexpositionallowsgeneticmanipulationsinvolvingfusionoruptakeofnucleicacidstobepossible .Theseadvantagesarelessachiev ablewhenusingintactcells (Liciaetal.,1999) .Incomparisonwithlandplantsandunicellularalgae ,on lylimitedrepo…     

不同景观条件下机械剥蚀与化学剥蚀间之比

机械剥蚀和化学剥蚀的数量是地形形成变化的主要指标,早在上一世纪末就已开始对其进行定量评定。通常,评定剥蚀的基础是离子径流和悬移泥沙径流。当认为这种方法是最客观和最精确时,还必须指出其极大局限性,这种局限性首先是由于机械剥蚀物和化学剥蚀物的形成与搬运的不同机制的缘故。     

坚定不移地加强党风廉政建设和反腐败工作——广西国土资源系统纪检监察工作会议综述

“要把党风廉政建设和反腐败工作作为国土资源管理工作的重要内容来抓,要以高度的政治责任感和坚持不懈的努力,与时俱进,开拓创新,力求在新思路、新突破、新举措上下功夫,努力开创我区国土资源管理和