The effects of electrochemical oxidation on in-vivo fluorescence and toxin content in Microcystis aeruginosa culture

The increasing occurrence of cyanobacterial blooms in water bodies is a serious threat to the environment. Efficient in-lake treatment methods for the control of cyanobacteria proliferation are needed, their in-vivo detection to obtain a real-time response to their presence, as well as the information about their physiological state after the applied treatment. In-vivo fluorescence measurements of photosynthetic pigments have proved to be effective for quantitative and qualitative detection of phytoplankton in a water environment. In the experiment, chlorophyll and phycocyanin fluorescence sensors were used concurrently to detect stress caused by electrochemical oxidation applying an electrolytic cell equipped with borondoped diamond electrodes on a laboratory culture of cyanobacteria Microcystis aeruginosa PCC 7806. The inflicted injuries were reflected in a clear transient increase in the phycocyanin fluorescence signal(for 104 %? 43%) 24 h after the treatment, which was not the case for the chlorophyll fluorescence signal. In the next 72 h of observation, the fluorescence signals decreased(on 40% of the starting signal) indicating a reduction of cell number, which was confirmed by cell count(24% reduction of the starting concentration) and analysis of extracted chlorophyll and phycocyanin pigment. These results demonstrate the viability of the combined application of two sensors as a useful tool for in-vivo detection of induced stress, providing real-time information needed for the evaluation of the efficiency of the in-lake treatment and decision upon the necessity of its repetition. The electrochemical treatment also resulted in a lower free microcystins concentration compared to control.     

Foreword

Ocean circulation in the Yellow and East China Seas is very rich in mesoscale phenomena, such as eddies, fronts, upwellings, river plumes, etc. Among others, having drawn tremendous attention from oceanographic community in the past for nearly 50 years are mainly the Yellow Sea Cold Water Mass (YSCWM) and its related circulation, the Yellow Sea Warm Current (YSWC), the East China Sea     

Influence of the Choice of Core-Envelope Transition Point on the Binary Merger of Two Main-sequence Components

We have studied the influence of different choices of core-envelope transition point on the final merger of contact binaries with two main-sequence components. A binary of 1.00 + 0.90M⊙ with an initial orbital period of 0.35d is examined. The mass fraction of the primary mixed with the matter of the secondary, qmix, determined by the chosen core-envelope transition point, ranges from 0.04 to 1.00 in our analysis. If as qmix< 0.8, none of the     

Geochemical survey of thermal system in Northeast China and their geothermometric characters

The chemical composition of several thermal springs around Changbaishan area has been investigated. Cenozoic basaltic rocks are widely distributed in Northeast China and geothermal characteristics have been described. About one hundred hot springs exist around Changhaishan Volcano at the border between China and D.P.R.Korea with high temperature about 82~C. The pH values of the spring water range from 6.9 to 7.1 and the total flow rate is about 4.8 L/sec. The chemical composition of the thermal springs is sodium carbonate; the high-mineral contents of thermal water are believed to have medicinal properties. Bathhouses are already built along the hot springs to take the advantage of the supposed healing properties. The high quality of those hot springs is believed to be utilitized for mineral water. The chemical equilibrium temperatures were estimated at about 160℃ based on the Na-K-Ca geochemical thermometer.     

GPS测量技术在海阳市城区控制中的应用研究

通过海阳市城区D级GPS控制网的布设，探讨了GPS控制网的布网方法和要求，阐述了GPS外业观测的质量控制措施，基线处理方法及其精度检验，讨论了已知控制点的选用方法，以及平差中应注意的问题等。     

Size-Flux Relation in Solar Active Regions

We present a study of the relationship between integral area and corresponding total magnetic flux for solar active regions. It is shown that some of these relationships are satisfied to simple power laws. Fractal examination showed that some of these power laws can not be justified inside the simple models of stationary magnetic flux tube aggregation. All magnetic fluxes and corresponding areas were calculated using the data measured with the Solar Magnetic Field Telescope of the Huairou Solar Observing Station in Beijing.     

Isolation of Protoplasts from Undaria pinnatifida by Alginate Lyase Digestion

1　Introduction　Protoplastsserveasabasicandversatiletoolforge neticengineeringandbiochemicalresearchforseveralreasons :theymayregeneratetheirwallsandprovideamodelsystemforstudyingwallbiogenesis ,theirlysisprovidesagentlemeansofpreparingsubcellularor ganelles ,andthemembraneexpositionallowsgeneticmanipulationsinvolvingfusionoruptakeofnucleicacidstobepossible .Theseadvantagesarelessachiev ablewhenusingintactcells (Liciaetal.,1999) .Incomparisonwithlandplantsandunicellularalgae ,on lylimitedrepo…     

不同景观条件下机械剥蚀与化学剥蚀间之比

机械剥蚀和化学剥蚀的数量是地形形成变化的主要指标,早在上一世纪末就已开始对其进行定量评定。通常,评定剥蚀的基础是离子径流和悬移泥沙径流。当认为这种方法是最客观和最精确时,还必须指出其极大局限性,这种局限性首先是由于机械剥蚀物和化学剥蚀物的形成与搬运的不同机制的缘故。     

苏联经济区方案定量研究的经验

通常,经济区划的任务包括以下几个主要阶段:1) 确定作为基础区的地域;2)按照基础区选择区划指标系统及其定量评价;3) 选择区域系统模式;4) 阐明课题建立算法,直接把基础区进行分类计算;5)解释已获得的结果,选出最好的区划方案和有关进一步改善工作方法的结论等等。基础区的范围视所要解决的任务性质而定。     

ANALYSIS OF DRIVING FORCES ON THE CHANGE OF HISTORICAL FARMLAND USE IN SHANDONG PROVINCE DURING THE QING DYNASTY (1644-1911)

1INTRODUCTIONTheIGBP-IHDP(InternationalGeosphere-BiosphereProgramme,andInternationalHumanDimensionPro-gramme)jointproject—LUCC(landuse/coverchange)isaninterdisciplinaryprogramdesignedtoimprovetheunderstandingofthedynamicsofland-useandland-coverchangeasinputstoandconsequencesofglobalenvironmentalchangeandsustainabledevelopment(CHARLOTTE,2002).Inparticularthedifferentbio-geochemicalmodeling(e.g.theglobalcarboncycle)ac-tivitiesandcomparativestudies,suchasthoseconduct-edbyIGBP-G…