The effects of electrochemical oxidation on in-vivo fluorescence and toxin content in Microcystis aeruginosa culture

The increasing occurrence of cyanobacterial blooms in water bodies is a serious threat to the environment. Efficient in-lake treatment methods for the control of cyanobacteria proliferation are needed, their in-vivo detection to obtain a real-time response to their presence, as well as the information about their physiological state after the applied treatment. In-vivo fluorescence measurements of photosynthetic pigments have proved to be effective for quantitative and qualitative detection of phytoplankton in a water environment. In the experiment, chlorophyll and phycocyanin fluorescence sensors were used concurrently to detect stress caused by electrochemical oxidation applying an electrolytic cell equipped with borondoped diamond electrodes on a laboratory culture of cyanobacteria Microcystis aeruginosa PCC 7806. The inflicted injuries were reflected in a clear transient increase in the phycocyanin fluorescence signal(for 104 %? 43%) 24 h after the treatment, which was not the case for the chlorophyll fluorescence signal. In the next 72 h of observation, the fluorescence signals decreased(on 40% of the starting signal) indicating a reduction of cell number, which was confirmed by cell count(24% reduction of the starting concentration) and analysis of extracted chlorophyll and phycocyanin pigment. These results demonstrate the viability of the combined application of two sensors as a useful tool for in-vivo detection of induced stress, providing real-time information needed for the evaluation of the efficiency of the in-lake treatment and decision upon the necessity of its repetition. The electrochemical treatment also resulted in a lower free microcystins concentration compared to control.     

广义物质平衡计算法在沙南凹陷BZ油田滚动勘探开发中的应用

渤海湾盆地沙南凹陷BZ油田沙一段生屑云岩主力含油储层投产后,发现实际生产特征与初始地质认识吻合度差,部分区块可能具有增储上产的潜力。通过利用油田生产动态资料,主要包括压力、产量及PVT实验等数据,采用广义物质平衡计算法识别出A4井区滚动勘探潜力最大。在此基础上,精细刻画生屑云岩沉积期古地貌和古生态环境特征并结合生屑云岩发育规律,预测了A4井区生屑云岩储层新的空间展布范围。实际钻探结果证实了储层预测的准确性,新增探明储量近400万t,保障了BZ油田的可持续发展和投资效益。该套方案对国内海上相似地质条件油田的滚动勘探开发具有借鉴意义。     

EFFECTS OF FISH OIL,DHA OIL AND LECITIHIN IN MICROPARTICULATE DIETS ON STRESS TOLERANCE OF LARVAL GILTHEAD SEABREAM（SPARUS AURATA）

The effects of natural fish oil,DHA oil and soybean lecithin in microparticulate diets on stress tolerance of larval gilthead seabream(Sparus aurata)were investigated after 15 days feeding trials.The tolerance of larval gilthead seabream to various stress factors such as exposure to air(lack of dissolved oxygen),changes in water temperature(low)and salinity(high) were determined.This study showed that microparticulate diet with natural fish oil and soybean lecithin was the most effective for in-creasing the tolerance of larval gilthead seabream to various stresses,and that microparticulate diet with natural fish oil and palmitic acid(16:0)was more effective than microparticulate diet with DHA oil and soybean lecithin.     

Geochemical survey of thermal system in Northeast China and their geothermometric characters

The chemical composition of several thermal springs around Changbaishan area has been investigated. Cenozoic basaltic rocks are widely distributed in Northeast China and geothermal characteristics have been described. About one hundred hot springs exist around Changhaishan Volcano at the border between China and D.P.R.Korea with high temperature about 82~C. The pH values of the spring water range from 6.9 to 7.1 and the total flow rate is about 4.8 L/sec. The chemical composition of the thermal springs is sodium carbonate; the high-mineral contents of thermal water are believed to have medicinal properties. Bathhouses are already built along the hot springs to take the advantage of the supposed healing properties. The high quality of those hot springs is believed to be utilitized for mineral water. The chemical equilibrium temperatures were estimated at about 160℃ based on the Na-K-Ca geochemical thermometer.     

Does sediment grain size affect diatom grazing by harpacticoid copepods?

Estuarine soft sediments support a diverse group of eukaryotic and prokaryotic organisms though the role of the sediment per se for the functioning of these organisms remains largely unknown. The present study aimed to test the effect of sediment grain size on the grazing activities of harpacticoid copepods. In controlled experiments, two common intertidal harpacticoid species (Paramphiascella fulvofasciata and Nitokra spinipes) were each offered a mix of two benthic diatom species (Navicula phyllepta and Seminavis robusta) in different sedimentary conditions. Several microcosms were created using a variety of sediment types, including fine silt (<63 microm), coarser grained sands (125-250, 250-450, 100-300 microm), artificial 'sediments' of glass beads (250-500, 2000 microm) and even the absence of sediment was tested. The diatoms were enriched in the stable carbon (13)C to facilitate tracing in the harpacticoids. Both copepod species were able to graze on the diatoms with highest uptake when sediment was absent. In contrast, both harpacticoid species showed no uptake in silty conditions. In general, grazing was favoured when mean sediment grain size increased. The strong negative effect of fine grains on the grazer's efficiency can be explained by the resulting differences in the structure (and accessibility) of the diatom biofilm on the one hand and the mobility of the grazer on the other hand. In view of the subtle equilibrium between primary producers and grazers, these results might have important implications for the effect of siltation of tidal flats due to, e.g., human activities.     

5个新发现的X选BL Lac天体

利用ＲＯＳＡＴＶＬＡ方法筛选，从ＲＯＳＡＴＸ射线源中选出了一批新的ＢＬＬａｃ天体和类星体的候选体．１９９６年１２月８日至１７日，利用北京天文台２．１６ｍ望远镜和新从美国引进的ＯＭＲ摄谱仪，对这批新候选体进行了光谱认证．经ＳＵＮ工作站处理，又发现了５个新的Ｘ选ＢＬＬａｃ天体．此外，还对去年作者在ＯＭＲ引进之前发现的ＢＬＬａｃ天体进行了检验，结果发现，去年发现的７个ＢＬＬａｃ天体中，２３２２＋３４３的ＣａＩＩＨ＆Ｋ“Ｂｒｅａｋｓｔｒｅｎｇｔｈ”为２６．９％，稍大于判据２５％，因此，２３２２＋３４３到底是ＢＬＬａｃ天体还是射电星系还有待于进一步测光及偏振观测的验证．     

地理现象的可分割性质

在过去几年里,分割的概念引起了人们的极大关注。其主要思想起源于地图数据研究,而且它的很多应用继续牵涉到空间现象。在对比例尺的答复、自相似和空间循环细分三个标题下,我们回顾了地理学与分割的关联。分割度大小为表现制图综合效果特性和评价以空间样本为预告对象所推出的数据表示提供了手段。分割表面自相似特性,使得它如同在地貌过程研究中开始的或不存在的假设景观一样有用。空间现象的广泛变化,据统计,在许多规模上被证明是自相似,使人想起了像地理范围那样的比例尺依存关系的重要性。在文章三分之一的篇幅里,循环细分被证明是导致用数字形式表示空间数据的新奇和有效方法,并成为空间组织常见模型的特征。我们断定,分割被作为考虑空间形式传统方法重要变化,同时,与其说是提供了空间现象新的和重要的规范与标准,倒不如说提供了以经验为根据的可证实模型。