ISOLATION AND CULTIVATION OF THE VEGETATIVE CELLS OF PORPHYRA HAITANENSIS

This research deals mainly with the use of the vegetative cells of a marine red alga Porphyra hailanensis (laver) as seeds and their culture into young thalli for further cultivation in the sea. The experimental process was as follows. Enzymatically isolated vegetative cells were attached to rope substrates and cultured in the laboratory for a month until they became about 0.2-0.5 cm long sporelings and were then attached (7-15 sporelings per cm of rope) to nets for removal to the open sea for cultivation. A month after culturing, the thalli reached a maximum length of 50 cm (average of 20-30 cm). The thalli grew faster as the water temperature dropped from 21℃ to 17℃. It was proved that the vegetative cells isolated from a small thallus about 5 cm long could grow up into normal thalli after being kept frozen for a year. The results of this study show that vegetative cells can be used as new seeds to simplify the production of laver sporelings. This technique is a significant advance in the field of P     

坛紫菜营养细胞和原生质体培养研究——Ⅱ.直接育苗下海养殖的实验研究

本文报道了王素娟等用坛紫菜(Porphyra haitanensis)营养细胞在苗绳上代替壳抱子采苗,并将幼苗下海养殖的实验。实验中,作者先酶解种藻成单离细胞,使附着于网绳上,在室内培养成O.2—0.5mm的幼苗,然后下海养殖,1个月后,幼苗长成了20—40cm的紫菜,藻体最长者可达58cm。结果表明:冷藏一年的2—5cm的幼苗解离细胞可以长成正常的商品紫菜。坛紫菜营养细胞可用作新苗源,它可以改革传统的生产程序,这在紫菜生产领域内具有重要意义。     

EARLY STAGE DIFFERENTIATION OF THALLUS CELLS OF PORPHYRA HAITANENSIS (RHODOPHYTA)

The early stage differentiation of thallus cells of Porphyra haitanensis T. J. Chang et B. F. Zheng was studied. Protoplasts or single cells were isolated from the blades using enzyme mixture comprising 2% sea snail gut enzyme and 1 % cellulase. The isolated protoplasts or single cells, were incubated in the MES medium. The cell differentiations were examined under the microscope at intervals after incubation. Four types of cell differentiation, namely, normal, abnormal, carposporangial and spermatorangial, and rhizoidal types, were observed. Since normal cell differentiations occur mostly in small thalli 50 mm in length and middle portions of big thalli 200 nun in length, it is essential to select tissues from these two kinds of thalli essential for commercial production.