The black-footed abalone Haliotis iris is an economically important shellfish species in New Zealand. We successfully amplified, sequenced and analysed the complete nuclear ribosomal DNA (nrDNA) of H. iris. The length of the nrDNA was determined to be around 9.6?kb and included, in order, small subunit ribosomal RNA (nrSSU, 1858bp), internal transcribed spacer (ITS, 749?bp), large subunit ribosomal RNA (nrLSU, 3412bp) and an intergenic spacer (IGS, 3560–3662?bp). The nrLSU genes were identical in two individuals, whereas the nrSSU and ITS regions existed at three and four base differences, respectively. The IGS was more variable than the other nrDNA regions. A phylogenetic tree was constructed based on the ITS sequence datasets, which revealed that Haliotidae has two major subclades, mainly distributed in the North Pacific, Europe and Australia. The complete nrDNA sequence will be useful for the classification, phylogeny and breeding of this shellfish. 相似文献
The transfer and evolution of stress among rock blocks directly change the void ratios of crushed rock masses and affect the flow of methane in coal mine gobs. In this study, a Lagrange framework and a discrete element method, along with the soft-sphere model and EDEM numerical software, were used. The compaction processes of rock blocks with diameters of 0.6, 0.8, and 1.0 m were simulated with the degrees of compression set at 0%, 5%, 10%, 15%, 20%, and 25%. This study examines the influence of stress on void ratios of compacted crushed rock masses in coal mine gobs. The results showed that stress was mainly transmitted downward through strong force chains. As the degree of compression increased, the strong force chains extended downward, which resulted in the stress at the upper rock mass to become significantly higher than that at the lower rock mass. It was determined that under different degrees of compression, the rock mass of coal mine gobs could be divided, from the bottom to the top, into a lower insufficient compression zone (ICZ) and an upper sufficient compression zone (SCZ). From bottom to top, the void ratios in the ICZ sharply decreased and those in the SCZ slowly decreased. Void ratios in the ICZ were 1.2–1.7 times higher than those in the SCZ.
Microwave Type III bursts with positive frequency drifting rate were found by Stahli and Benz (1987) for first time. Type III events are especially interesting because they are well-known to be signatures of electron beams in coronal plasma, and they are effective means for diagnosting of source plasma. A microwave burst consisting of some microwave type III burst groups was registered at Beijing Astronomical Observatory with the 2545–2645–2840 MHz synchronous observing system. The distributions of frequency drifting rate, half power duration, and intensity for each impulse in the groups have been statistically analysed. From this analysis, some important parameters for the dynamic process in the flare are deduced and discussed. 相似文献