Application of the first internal transcribed spacer(ITS-1) of ribosomal DNA as a molecular marker to population analysis in farrer''s scallop Chlamys farreri

Sequence variation of the first internal transcribed spacer of ribosomal DNA （ ITS - 1 ） was examined and its application to the study of genetic variation was explored in four populations of farter＇ s scallop Chlamys farreri. ITS - 1 fragments, with a length of about 300 bp,of 78 individuals collected from Dalian, Qingdao, Yantai in China and Korea respectively were amplified via PCR, cloned and sequenced. Intra-genomic variation was examined by sequencing several clones of single individuals. Alignment and polymorphism analysis detected 44 haplotypes and 50 polymorphic sites which consist of 30 substitutions and 20 indels, indicating a high level of polymorphisms. Sequence analysis also showed a very low level of intra-individual variation. All these features validated the feasibility of application of ITS - 1 fragment to population analysis. Polymorphism analysis showed that the Korea sample has the richest genetic variation, followed by Yantai and Qingdao samples. AMOVA （analysis of molecular variance） showed that the majority （96.26%） of genetic variation was distributed within populations and 3.74% resulted from among populations, but with P 〈 0.05 （ = 0.042）, indicating that the populations in this study have significant divergence. This output was basically concordant with the result arising from RAPD data and different from that from mitochondrial 16S rDNA sequence data. Discussion on this inconsistency was made accordingly.     

精品课程教学基本建设与教学改革研究——以《遥感概论》省级精品课程建设为例

以《遥感概论》省级精品课程建设为例,探讨精品课程教学基本建设与教学改革。认为高素质的教师队伍是精品课建设的基础,课程体系和教学内容的设计是精品课建设的关键,课程网站是精品课程建设的必备条件,学生综合能力提高是精品课建设的目标,并结合课程教学实践,提出了相应的建设措施。     

基于AutoCAD的管线调查系统的建设

现有管线调查常用的记录方式有直接用笔记录于调查表格中和使用PDA作为管线外业调查的手簿两种方式,这两种方式都需要大量的内业数据处理。为提高管线调查的效率,进行了基于AutoCAD的管线调查系统的建设。本文介绍了该系统的开发方式,系统功能,作业流程等。着重介绍了管线调查信息的存储、管线信息调查、自动生成调查井位、自动生成高程表和调查表等功能以及操作命令的制作等关键技术。该系统已进行了试点应用,实践证明大大提高了作业效率,取得了良好的效果。     

青藏高原腹地湖泊沉积对第四纪晚期古季风变化的响应

通过对青藏高原腹地的综合科学考察和对中心钻孔岩芯剖面的最新研究,用层序地层学与年代地层学和气候地层学相结合的方法,分辨出可可西里地区湖泊沉积记录(孔深7.25 m)的第四纪晚期距今3万余年以来的古气候变化,沉积物磁化率等因子综合表征的高原古季风变化是波动发展的,发生在仙女木期地质环境事件中的季风活动具有强烈暴发的特点,是高原季风发展中的突变事件,而且地表热点效应对其起到了激发作用。综合分析的研究成果表明,它的变化频谱与激变因子及其运行机制是伴随着青藏高原地质效应的演变而发展的,为研究第四纪冰消期以来的气候变化提供了新的信息。     

Homogenization of the Daily Land Surface Temperature over the Mainland of China from 1960 through 2017

Land surface temperature(LST) is one of the most important factors in the land-atmosphere interaction process. Raw measured LSTs may contain biases due to instrument replacement, changes in recording procedures, and other non-climatic factors. This study attempts to reduce the above biases in raw daily measurements and achieves a homogenized daily LST dataset over China using 2360 stations from 1960 through 2017. The high-quality land surface air temperature(LSAT)dataset is used to correct the LST warming biases especially evident during cold months in regions north of 40°N due to the replacement of observation instruments around 2004. Subsequently, the Multiple Analysis of Series for Homogenization(MASH) method is adopted to detect and then adjust the daily observed LST records. In total, 3.68 × 103 effective breakpoints in 1.65 × 106 monthly records(about 20%) are detected. A large number of these effective breakpoints are located over large parts of the Sichuan Basin and southern China. After the MASH procedure, LSTs at more than 80% of the breakpoints are adjusted within +/– 0.5°C, and of the remaining breakpoints, only 10% are adjusted over 1.5°C.Compared to the raw LST dataset over the whole domain, the homogenization significantly reduces the mean LST magnitude and its interannual variability as well as its linear trend at most stations. Finally, we perform preliminary analysis upon the homogenized LST and find that the annual mean LST averaged across China shows a significant warming trend [0.22°C(10 yr)–1]. The homogenized LST dataset can be further adapted for a variety of applications(e.g.,model evaluation and extreme event characterization).     

云南个旧卡房铜多金属矿床流体包裹体特征及其地质意义

卡房铜多金属矿床位于云南省个旧锡多金属矿集区, 作为该区南段典型的铜多金属矿床, 其深入的成矿流体性质及成矿物质来源研究尚为薄弱, 限制了对该矿床成因的深入认识。本文对产于玄武岩中的似层状铜多金属矿体(主要成矿类型)开展了精细的野外岩相学、矿物学、C-H-O同位素及流体包裹体原位成分分析。结果显示, 流体包裹体主要为气液两相水溶液包裹体, 石英流体包裹体均一温度为 264.5~330.5 ℃、盐度w(NaCleq)为16.24%~18.20%、密度为0.853~0.924 g/cm3; 萤石流体包裹体均一温度为199.8~339.1 ℃、盐度w(NaCleq)为8.81%~18.88%、密度为0.822~0.955 g/cm3; 方解石流体包裹体均一温度为112.1~199.4 ℃、盐度w(NaCleq)为4.96%~10.86%、密度为0.910~1.011 g/cm3, 表明卡房铜多金属矿床成矿流体具有中低温、中低盐度、低密度特征, 且早阶段到晚阶段, 成矿温度和流体盐度明显降低。与Cu矿共生的石英δDV-SMOW值为–83.9‰ ~ –78.9‰, δ18OH2O值为4.63‰~12.14‰, 方解石δ13CV-PDB值为–7.11‰~ 0.64‰; 同时, 石英单个流体包裹体原位分析结果显示流体包裹体富集大离子亲石元素, 如Na、K、Rb、Cs、Sr等。这些特征暗示了流体可能来源于与花岗质岩浆有关的热液流体, 热液流体上升过程中与围岩碳酸盐岩发生强烈的水-岩反应; 该过程可能萃取了玄武岩层中的Cu物质, 诱发了Cu的富集并最终成矿。     

云南金沙厂铅锌矿床地球化学特征及其指示意义

位于扬子地块西南缘的“川滇黔铅锌矿集区”是我国西南大面积低温成矿域的重要组成部分, 金沙厂铅锌矿床是其中典型矿床之一, 前人针对金沙厂矿床的成矿物质来源和成矿流体特征开展了大量研究, 但围岩对成矿的贡献和矿床成因仍不明确。针对这一问题, 本文系统分析了金沙厂闪锌矿及围岩的微量元素组成, 结合围岩和萤石的稀土元素特征, 探讨了围岩对成矿的贡献, 厘定了矿床成因。研究表明, 闪锌矿以富集Cd、Ge、Cu、Ga, 贫Fe、Mn、In、Co、Ni为特征, 这些元素多以类质同象的形式赋存在闪锌矿中, 个别元素如Cu、Ge等含量变化范围较大, 可能与低温流体有关。矿床中闪锌矿存在多种颜色的原因可能是Cu、Ge等多种元素共同作用的结果。近矿端的围岩明显富集Zn、Pb、As、Cd等元素, 而闪锌矿中同样富集这些元素, 说明围岩为成矿提供了部分金属元素。萤石继承了围岩的Eu、Ce负异常, 并受到了具有较高Ce负异常的成矿流体影响。萤石较围岩具有较高的Y/Ho比值, 说明萤石中的部分Y来源于围岩, 从而导致萤石具有较高的Y/Ho值。总体上, 该矿床中闪锌矿微量元素组成与MVT矿床基本一致, 明显有别于喷流沉积型、岩浆热液型和远端夕卡岩型矿床, 其成矿温度属于低温范围, 结合其矿床地质地球化学特征, 本文认为金沙厂铅锌矿床属于MVT铅锌矿床。     

RP-HPLC法同时测定翅碱蓬中四种黄酮化合物的含量

建立RP-HPLC(反相高效液相色谱法)同时测定翅碱蓬中四种黄酮化合物-芸香苷、槲皮素3-β-D-葡萄糖苷、水仙苷和槲皮素的含量。采用Kromasil C18色谱柱(4.6 mm×250 mm, 5μm),检测波长为361nm,柱温为30℃,流动相为甲醇-0.4%冰乙酸(55︰45),流速1.0mL/min,四种黄酮类化合物得到很好的分离。芸香苷、槲皮素3-β-D-葡萄糖苷、水仙苷和槲皮素浓度均为0.001～0.01 mg/mL范围内呈现良好线性关系,相关系数R2在0.999 0～0.999 6,加标回收率(n=3)范围为82.39%～99.03%。该方法简单、快速、高效,可以用于翅碱蓬中四种黄酮化合物的含量分析。     

姑婆山复式岩体的基本特征及其与成矿作用的关系

姑婆山复式岩体由晚侏罗世里松岩体、姑婆山岩体及早白垩世新路岩体组成。复式岩体为壳幔混合源成因。据岩石地球化学判断和分析,姑婆山岩体和新路岩体为含锡岩体;整个姑婆山复式岩体为含钨岩体,里松岩体和姑婆山岩体为含稀有、稀土岩体。上述矿产的形成与岩石中挥发份F、B及放射性U、T h等元素的高含量有关。     

利用扫描电镜、压汞法、氮气吸附法评价近海黏土孔隙特征

近海地区黏土的微观结构单元--黏土畴中含有大量的粒内孔隙,其孔径小至纳米级,这部分孔隙对黏土的物理指标、水稳性、膨胀性、收缩性等具有重要影响,一般方法很难准确地描述其孔隙特征。试验利用扫描电镜、压汞法、氮气吸附法对湛江黏土的微观孔隙特征及其孔隙发育的控制因素分析,建立微观结构与物理指标、力学行为的相互关系与灵敏性分析。结果表明,湛江黏土具有不良物理性质和良好力学特性指标的异常组合,是一种高灵敏性的强胶结结构性黏性土,其机制主要是：湛江黏土的微观结构为带有胶质联结特性的、定向性无序的开放式絮凝结构,孔隙结构为具有较高的强度和空间稳定性的边－面－角联结的空间网架系统。由于特殊的孔隙网架结构以及“墨水瓶”型孔隙的存在,导致退汞过程出现滞留现象及产生吸附回线。研究表明,联合扫描电镜、压汞法、氮气吸附法能够准确、完整地对近海地区黏土孔隙体系特征进行定性与定量的评价。