Enhanced a novel β-agarase production in recombinant Escherichia coli BL21(DE3) through induction mode optimization and glycerol feeding strategy

Agarases are hydrolytic enzymes that act on the hydrolysis of agar and have a broad range of applications in food,cosmetics and pharmaceutical industries. In this study, a glycerol feeding strategy based on induction mode optimization for high cell density and β-agarase production was established, which could effectively control acetate yield. First, exponential feeding strategy of glycerol with different overall specific growth rates(μ) was applied in the pre-induction phase. The results showed that the low μ(μ=0.2) was suggested to be the optimal for cell growth and β-agarase production. Second, the effects of induction temperature and the inducer concentration on cell growth and β-agarase production were investigated in the post-induction phase. When induced by isopropyl-β-d-thiogalactoside(IPTG), the strategy of 0.8 mmol/L IPTG induction at 20℃ was found to be optimal for β-agarase production. When cultivation was induced by continuous lactose feeding strategy of 1.0 g/(L·h), the β-agarase activity reached 112.5 U/mL, which represented the highest β-agarase production to date.Furthermore, the β-agarase was capable of degrading G. lemaneiformis powder directly to produce neoagarooligosaccharide, and the hydrolysates were neoagarotetraose(NA4) and neoagarohexaose(NA6). The overall research may be useful for the industrial production and application of β-agarase.     

敦煌-格尔木铁路沿线风动力环境特征

敦煌-格尔木铁路沿线地形复杂、起沙因素多变、沙源丰富，沙害问题日益严重。目前对其风沙活动规律还未有研究，不利于防沙工作的开展。为此，通过对自北向南的5个观测点（S1、S2、S3、S4、S5）风速和风向的观测、计算和分析，利用平均风速、起沙风况及输沙势对敦格铁路沿线的风动力环境特征进行研究。结果表明：S5、S4和S3的风况对铁路风沙灾害防治意义较大。S5年平均风速、起沙风频率和输沙势最大，春季风沙活动最为强烈，且风向单一、风力强劲，风沙运动方向基本与铁路垂直，沙粒易在铁路附近堆积。S4夏季风沙活动最为强烈；S3春季风沙活动最为强烈，且风向单一，S4和S3的风沙运动方向与铁路夹角小于90°，附近沙源广阔，铁路易受风沙侵蚀，阻碍交通运营。     

Finding KBO Flyby Targets for New Horizons

Development of the New Horizons mission to Pluto and the Kuiper Belt is now fully funded by NASA (Stern and Spencer, this volume). If all goes well, New Horizons will be launched in January 2006, followed by a Jupiter gravity assist in 2007, with Pluto arrival expected in either 2015 or 2016, depending on the launch vehicle chosen. A backup launch date of early 2007, without a Jupiter flyby, would give a Pluto arrival in 2019 or 2020. In either case, a flyby of at least one Kuiper Belt object (KBO) is planned following the Pluto encounter, sometime before the spacecraft reaches a heliocentric distance of 50 AU, in 2021 or 2023 for the 2006 launch, and 2027 or 2029 for the 2007 launch. However, none of the almost 1000 currently-known KBOs will pass close enough to the spacecraft trajectory to be targeted by New Horizons, so the KBO flyby depends on finding a suitable target among the estimated 500,000 KBOs larger than 40 km in diameter. This paper discusses the issues involved in finding one or more KBO targets for New Horizons. The New Horizons team plans its own searches for mission KBOs but will welcome other U.S, or international team who wish to become involved in exchange for mission participation at the KBO.     

山东温泉旅游资源分析及科学发展思考

山东省温泉资源分布广泛、储量大，利用历史十分悠久，在温泉资源开发应用过程中，应根据其分布、类型、开发利用现状，并结合国内外的科技成果而进行科学开发与综合利用，建设既有地方民俗文化特色又与国际接轨的可持续发展模式。     

由两块拼接反射镜组成的光学系统的衍射能量分布

LAMOST的光学系统由反射施密特改正板MA 和球面主镜MB 组成 ,MA 和MB 均为对角径 1 .1m的六角形子镜的拼镜面 .本文计算了星在子午圈上时望远镜观测不同的天区零视场和有视场时的光学系统衍射能量分布 ,还计算了MA 不变MB 改为整块的 6m镜以及MA 和MB 均为对角径 1 .5m的六角形子镜的拼镜面的衍射能量分布 .对这些计算结果进行分析 ,论证了六角形子镜尺寸选择的合理性     

桶形基础平台三维有限元稳定性分析

以我国第一座桶形基础采油平台工程实例为原型，采用三维有限元计算方法对桶形基础稳定性分析。平台在波浪、风等荷载作用下，其稳定性不仅与平台的整体结构有关，而且与桶形基础及其周围土体相互作用相关。在计算中，利用薄层摩擦单元模拟土与结构间的相互作用，取得了很好的效果。分析结果表明桶形基础采油平台在设计条件下运行是较为稳定的。     

Threshold condition of sand particles under codirectional combined wave and current flow

Flume experiments were carried out to determine the threshold of movement of sand on rippled surface and flat beds under the codirectional combined flows due to waves and currents. The results indicate that Shields's curve can be used to determine the threshold condition under combined flows, provided that the maximum bed shear stress is used.     

Evidence for sinistral strike-slip deformation in The Solomon Island arc

During the SOPACMAPS 2 crusie carried out by IFREMER (Institut Français de Recherche pour l'Exploitation de la Mer) and ORSTOM (Institut Français de Recherche Scientifique pour le dévelopement en Coopération) on theR/V L'Atalante, in the Central Solomon Arc area, multibeam bathymetric and imagery data and single-channel seismic reflection profiles were collected from an area of about 3500 km², to evaluate regional tectonics. Structural data geophysical profiles interpretation provide evidence for left-lateral transtensional tectonics on the southern edge of the Central Solomon Trough. This transtensional deformation is represented by faulting, block tilting, and rhombohedral deformation. The regional geology and the analysis of the sedimentary cover allow us to demonstrate that this tectonic occurred in two different phases during Oligocene to Miocene and Pliocene to Pleistocene times.     

台湾海峡毛颚动物分布的初步研究

本文分析了台湾海峡毛颚动物的种类组成、数量分布特征，并就其与环境因素的关系进行了初步探讨．结果显示与台湾海峡西部、东海等邻近海域相比，本区毛颚动物具种数少，数量小，季节变化幅度较小等特点．它的数量分布状况与闽斯沿岸流、南海西南季风漂流以及黑潮支流在本区运动消长有关。     

埕岛油田海上石油平台基础冲刷研究

海洋石油平台的建立会改变平台周围的水动力条件，其中平台的基础及与之连接的海底管缆(输油管道、注水管道、海底电缆等)附近产生冲刷现象，对平台和海底管缆的安全造成威胁。根据对研究区典型平台的4次实洲水深并结合以往资料，分析了埕岛油田海上石油平台基础冲刷的过程及其规律。