强光照和黑暗条件下刺参体壁的基因表达差异

参对光照变化非常敏感，研究刺参对光照的分子响应非常重要。本研究应用RNA测序获取了刺参暴露于强光（“强光”）、正常光照（“对照”）和完全黑暗（“黑暗”）环境下体壁的基因表达谱情况，通过“对照”与“黑暗”，“对照”与“强光”和“黑暗”与“强光”的比较，在|log₂ ratio|≥1和FDR≤0.001的标准下分别发现了1161、113和1705个差异表达基因（DEGs）。基因本体分析表明，“cellular process”和“binding”在“生物过程”和“分子功能”类别中的DEGs富集最多，而“cell”和“cell part”在“细胞组分”类别中的DEGs富集最多。将DEGs与Kyoto Encyclopedia基因和基因组数据库上的于214、41和229条通路进行比对，发现了51、2和57条通路分别显著富集。本研究发现的光特异性DEGs可作为深入研究刺参对光照变化的生化适应机制的重要目标基因。     

Geochemistry and environmental effect of cadmium in the super-large Jinding Pb-Zn deposit,Yunnan Province,China

The Jinding Pb-Zn deposit in Yunnan Province is the representative of a Cd-enriched area and mining activities lead to the release of Cd into the hypergenic ecosystem, resulting in Cd pollution. The concentrations of Cd vary greatly from one type to another type of rocks in the mining district. In the host rock, Cd concentrations range from 50×10^-6 to 650×10^-6 with an average of 310×10^-6. In primary ores, Cd concentrations range from 14×10^-6 to 2800×10^-6 with an average of 767×10^-6. However, in oxidized ores, Cd concentrations are highest, varying within the range of 110×10^-6 to 8200×10^-6 , averaging 1661×10^-6. It is shown that the oxidized ores are the main carder and environmental source of Cd. Leaching test showed that Pb/Zn ores are easy to oxidize and thereafter release Cd and other harmful elements. These leached elements in the leachate may be precipitated rapidly in the order of Zn〉Pb〉Cd. As for the concentration distribution of Cd in the Bijiang River, it is estimated to be 15.7 μg/L Cd in water, 49.3 mg/L in suspended substances, and 203.7 mg/L in sediments. The average value of Cd in soil from the polluted area is 83.0 mg/kg. Natural weathering of Cd-rich rocks and minerals imposes a potential environmental risk on the aquatic ecosystem of the Bijiang catchment.     

Differential gene expression in the body wall of the sea cucumber(Apostichopus japonicus) under strong lighting and dark conditions

Sea cucumber, Apostichopus japonicus is very sensitive to light changes. It is important to study the influence of light on the molecular response of A. japonicus. In this study, RNA-seq provided a general overview of the gene expression profiles of the body walls of A. japonicus exposed to strong light("light"), normal light("control") and fully dark("dark") environment. In the comparisons of "control" vs. "dark", "control" vs. "light" and "dark" vs."light", 1 161, 113 and 1 705 differentially expressed genes(DEGs) were identified following the criteria of|log_2 ratio|≥1 and FDR≤0.001, respectively. Gene ontology analysis showed that "cellular process" and "binding"enriched the most DEGs in the category of "biological process" and "molecular function", while "cell" and "cell part" enriched the most DEGs in the category of "cellular component". And the DEGs were mapped to 214, 41 and229 pathways in the Kyoto Encyclopedia of Genes and Genomes database, and 51, 2 and 57 pathways were significantly enriched, respectively. Light-specific DEGs identified in this study will be important targets for further investigation to establish the biochemical mechanisms involved in the adaption of this sea cucumber to changes in the level of environmental light.     

基于扎根理论与文本分析的海洋旅游目的地意象研究

旅游目的地意象在旅游者决策与目的地开发中具有重要作用，也是旅游研究中的热点领域。海洋旅游目的地意象的研究对于提高目的地竞争力与吸引力具有重要意义。本文以夏威夷为例，基于国内旅游者发布在网络上的游记，利用扎根理论与文本分析法对夏威夷的认知意象、情感意象与总体意象进行分析。研究发现，“海洋自然吸引物”、“海洋人文吸引物”、“海洋旅游活动”、“目的地软环境”、“旅游服务”、“公共场所与服务”是构成海洋旅游目的地认知意象的六个主范畴；在此基础上，通过提取游记中的情感语句并结合“情感环状模型”发现夏威夷的情感意象以正面情感为主；从语义网络与重游意愿来看，夏威夷的总体意象较为积极，并未出现明显的负面感知要素。研究旨在为国内海洋旅游目的地的发展提供一定的经验与借鉴。     

安徽省硫铁矿成因类型及潜力分析

安徽省是我国硫铁矿储量大省,截止2013年底,全省累计查明非伴生硫铁矿9.1亿吨,保有资源储量8.2亿吨,居全国第二位,华东第一位。     

安徽滁州地区闪长质岩锆石U-Pb年龄与地球化学:岩石成因和动力学意义

用LA-ICP-MS测得安徽滁州2个闪长玢岩样品中锆石~(206)Pb/~(238)U年龄为126.19±0.44Ma和126.4±0.7Ma,结合前人研究,得出滁州地区岩体的侵位时代应为120~130Ma之间,为早白垩世。岩石地球化学研究显示,Si O_2含量变化范围为56.75%~60.90%,具有高Al_2O_3(14.82%~15.77%)、Mg O(4%)、Sr(750×10~(-6))、Sr/Y(62~110)、La/Yb(20~36),低Y、Yb的特征,同时富集轻稀土元素和大离子亲石元素,亏损高场强元素,Eu异常不明显,属于典型的埃达克质岩。Mg~#值为39~45,K_2O/Na_2O值为0.57~0.96,平均值为0.75,明显低于大别造山带加厚下地壳埃达克岩,Ce/Pb值较低,大多集中在3~5之间,类似于陆壳而明显低于洋壳。研究认为,安徽滁州地区埃达克质岩由拆沉下地壳部分熔融形成,埃达克质岩浆在上升过程中与地幔橄榄岩发生反应,导致熔体Mg O、Cr、Ni等含量增加。早白垩世中国东部地壳伸展减薄导致下地壳拆沉,地幔物质的参与带来铜、金等成矿物质,埃达克质岩可作为该地区重要的找矿标志。     

Study of intensity fluctuations in cosmic rays during Forbush-decreases on the basis of the data obtained with the inzmiran scintillation supertelescope

The one-minute, five-minute, and hourly values of cosmic-ray intensity obtained with a scintillation telescope at Izmiran have been used to carry out the correlational and spectral analysis of large Forbush-decreases of cosmic rays in 1978. The spectra of power obtained for the periods of interplanetary disturbances are characterized by individual spectral lines whose amplitude exceeds at least a 95% interval of reliability.     

Hydrogeochemical processes of thallium in a rural area of Southwest Guizhou, China

The water system in a rural area of Lanmuchang in Southwest Guizhou is facing a risk of thallium (Tl) contamination due to Tl mineralization around the area. The major trace elements and Tl in the water system are studied to understand the hydrogeochemical processes of Tl constrained by Tl mineralization. The results showed that the dispersion pattern of Tl follows a descending order in concentration from mine groundwater (deep groundwater) →stream water→shallow groundwater→background water, reflecting the impact of Tl mineralization on the hydrogeochemical composition. Tl concentrations in stream water in both regimes are remarkably higher (2-30 fold) downstream than up- and mid-streams, probably caused by the unidentified discharge of deep groundwater. Low Tl levels are detected in the current drinking water, however, the highly elevated Tl in stream water and ground water may pose a potential environmental risk through daily washing and agricultural irrigation. This study suggests that human activities, such as agricultural irrigation, could intensify the environmental risk of Tl.     

Evaluation of body weight of sea cucumber Apostichopus japonicus by computer vision

Apostichopus japonicus(Holothuroidea,Echinodermata) is an ecological and economic species in East Asia.Conventional biometric monitoring method includes diving for samples and weighing above water,with highly variable in weight measurement due to variation in the quantity of water in the respiratory tree and intestinal content of this species.Recently,video survey method has been applied widely in biometric detection on underwater benthos.However,because of the high flexibility of A.japonicus body,video survey method of monitoring is less used in sea cucumber.In this study,we designed a model to evaluate the wet weight of A.japonicus,using machine vision technology combined with a support vector machine(SVM) that can be used infield surveys on the A.japonicus population.Continuous dorsal images of free-moving A.japonicus individuals in seawater were captured,which also allows for the development of images of the core body edge as well as thorn segmentation.Parameters that include body length,body breadth,perimeter and area,were extracted from the core body edge images and used in SVM regression,to predict the weight of A.japonicus and for comparison with a power model.Results indicate that the use of SVM for predicting the weight of 33 A.japonicus individuals is accurate(R~2=0.99) and compatible with the power model(R~2=0.96).The image-based analysis and size-weight regression models in this study may be useful in body weight evaluation of A.japonicus in lab and field study.