Chromosome Behavior of Heat Shock Induced Triploid in Fenneropenaeus Chinensis

INTRODUCTIONTriploidanimalsareusefulbecausetheyaresterile ,characterizedbypositivegrowthinthere productiveseason ,usefulfortesting ,forintroductionofnon nativespecies ,andforprotectionofde velopedstrains.Theinductionoftriploidyhadbeenreportedinmanyaquaculturespecies .Triploidshavebeensuccessfullyinducedinshrimpusingtemperatureshockorchemicalshock (CB ,6 DMAP)in 4species :Sicyoniaingentis (Xiangetal.,1 991 ) ,Fenneropenaeuschinensis (Xiangetal.,1 992 ;Daietal.,1 993;Baoetal.,1 993;Li…     

中国对虾(Penaeus chinensis)四倍体诱导研究

利用光控和行为学原理,控制中国对虾在适当时候产卵,在一定的时刻以温度休克和细胞松弛素的方法诱导受精卵发育成为四倍体。作者发展了对虾染色体制备技术,以中肠和触角腺、精巢为材料,从后期幼体直到8～9cm左右次成虾均获得较好的分裂中相。染色体倍性检测结果表明,最好的四倍体诱导成功率达66.7％。共获得10cm左右的实验对虾几千尾。初步观察表明,四倍体中国对虾具有一定的生长优势。     

基于自动观测站的鲁中地区土壤水分变化规律及精细化预报模型的研究

为了建立鲁中地区土壤水分精细化预报模型,利用2010—2013年农田土壤水分自动站逐日资料进行土壤水分年、月变化特征研究,并结合附近自动气象站资料,以土壤水分平衡方程、农田蒸散模型为基础,采用逐步回归和曲线估计等方法建立4—6月无降水条件下平原水浇田与山旱田土壤水分1 d、7 d降幅的经验预报模型。结果表明:鲁中地区0~100 cm土壤水分贮存量年变化趋势和0~50 cm基本一致,年最高出现在8月,最低出现在6月,年降幅最大出现在3—6月,易出现干旱。对预报模型进行回代和预报检验结果显示,回代平均相对误差为0.07%,7 d模型和1 d模型滚动预报第7天0~50 cm土壤水分贮存量,绝对误差分别为-0.15和-2.17 mm,平均相对误差分别为-0.07%和-1.56%,模型具有较强的理论基础和实用性,预报精度较高,为鲁中地区土壤墒情监测和精细化预报提供支持。     

驼背鲈引种驯养及人工诱导性腺发育和繁殖

为探讨通过人工诱导获得驼背鲈成熟亲鱼的途径，在室内水池进行驼背鲈驯养研究；用口服和注射性激素、控温、控光、营养强化、模拟生态条件等方法处理驼背鲈以诱导其性腺发育。结果表明，控光和模拟生态条件不能有效诱导性腺发育；激素、控温和营养强化相结合可诱导驼背鲈性腺发育并成熟。对其中１ 组诱导成熟的亲鱼（雌、雄各１ 尾）进行人工催产后，亲鱼自然产卵受精，孵出仔鱼２ 万尾。驼背鲈在驯养条件下生长较快；通过人工控制和诱导可达到性腺发育和成熟，性腺发育速度较快。     

Molecular markers for identifying a new selected variety of Pacific white shrimp Litopenaeus vannamei

Selective breeding of the Pacific white shrimp Litopenaeus vannamei during the last decade has produced new varieties exhibiting high growth rates and disease resistance.However,the identification of new varieties of shrimps from their phenotypic characters is difficult.This study introduces a new approach for identifying varieties of shrimps using molecular markers of microsatellites and mitochondrial control region sequences.The method was employed to identify a new selected variety,Kehai No.1(KH-1),from three representative stocks(control group):Zhengda;Tongwei;and a stock collected from Fujian Province,which is now cultured in mainland China.By pooled genotyping of KH-1 and the control group,five microsatellites showing differences between KH-1 and the control group were screened out.Individual genotyping data confirmed the results from pooled genotyping.The genotyping data for the five microsatellites were applied to the assignment analysis of the KH-1 group and the control group using the partial Bayesian assignment method in GENECLASS2.By sequencing the mitochondrial control regions of individuals from the KH-1 and control group,four haplotypes were observed in the KH-1 group,whereas14 haplotypes were obtained in the control group.By combining the microsatellite assignment analysis with mitochondrial control region analysis,the average accuracy of identification of individuals in the KH-1group and control group reached 89%.The five selected microsatellite loci and mitochondrial control region sequences were highly polymorphic and could be used to distinguish new selected varieties of L.vannamei from other populations cultured in China.     

Effect of salinity on growth and first sexual maturity of Exopalaemon carinicauda (Holthuis, 1950)

To determine the optimal salinity for growth and first sexual maturity of ExopaIaemon carinicauda, the effects of salinity on growth and reproductive performance of early juvenile prawns of E. carinicauda were evaluated under laboratory conditions. Postlarvae from the same female broodstock were reared at six salinity levels （5, 10, 15, 20, 25, and 30） for 12 weeks. The specific growth rate （SGR） and survival rate （SR） under different salinity levels in the first 6 weeks were calculated and compared. SGR was significantly influenced by salinity. Prawns reared in salinity of 10 grew significantly faster （P〈0.05） than those reared in salinities of 5, 20, 25, and 30. However, ANOVA confirmed that there was no significant effect among the six salinity levels on SR. For the next 6 weeks, the body length （BL）, body weight （BW） at the first sexual maturity, and the age at median sexual maturity （As0） of females were measured and compared. Female prawns reared at salinity of 10 presented significantly shorter A50, but no significant differences among the six salinity levels for BL and BW at the first sexual maturity were observed. Based on the above information, the optimal salinity for growth and first sexual maturity of juvenile E. carinicauda is approximately 10.     

Construction of bacterial artificial chromosome libraries for Zhikong Scallop Chlamys farreri

Two Large-insert genomic bacterial artificial chromosome （BAC） libraries of Zhikong scallop Chlamys farreri were constructed to promote our genetic and genomic research. High-quality megabase-sized DNA was isolated from the adductor muscle of the scallop and partially digested by BamH I and Mbo I, respectively. The BamH I library consisted of 53 760 clones while the Mbo I library consisted of 7 680clones. Approximately 96 % of the clones in BamH I library contained nuclear DNA inserts in average size of 100 kb, providing a coverage of 5.3 haploid genome equivalents. Similarly, the Mbo I library with an average insert of 145 kb and no insert-empty clones, thus providing a genome coverage of 1.1 haploid genome equivalents.     

大兴安岭地区加密观测前后气象资料的对比分析

1 引言 黑龙江省大兴安岭地区面积8.46×104km2,在2006年以前大兴安岭地区气象部门有6个地面气象观测站,平均1.41×104km2有一个地面观测站,气象站在空间密度和观测频次上,不能适应中小尺度天气系统的监测要求.目前,大兴安岭地区共有52个观测站覆盖在大兴安岭的所有乡镇、林场,平均30 km有1个雨量站.实行了1 h一次的自动上传气温、雨量数据.通过对2007年1～9月的加密观测前后资料的对比分析,揭示了大兴安岭地区的气候变化规律,为林区的气象服务提供科学的依据.     

脾胃湿热证与气象要素相关性初探

利用湖南中医药大学附属二医院2008年消化内科脾胃疾病湿热内蕴证与同期长沙市气象要素观测资料,通过回归分析,对脾胃疾病湿热内蕴证与气象要素的相关性进行了研究。结果表明,湿热内蕴证与气温、降水量、相对湿度呈正相关,与气压呈负相关,与风无显著相关。     

Structural analysis of a shrimp thymidylate synthase reveals species-specific interactions with dUMP and raltitrexed

Journal of Oceanology and Limnology - Thymidylate synthase (TS) is a key enzyme in the de novo biosynthesis of thymidine monophosphate, serving as a well-known drug target in chemotherapy against...