Fast genetic mapping in barley: case studies of cuticle mutants using RNA-sequencing

Barley(Hordeum vulgare L.) is one of the earliest domesticated crop species and ranked as the fourth largest cereal production worldwide. Forward genetic studies in barley have greatly advanced plant genetics during the last century; however, most genes are identified by the conventional mapping method. Array genotyping and exome-capture sequencing have also been successfully used to target the causal mutation in barley populations, but these techniques are not widely adopted because of associated costs and partly due to the huge genome size of barley. This review summarizes three mapping cases of barley cuticle mutants in our laboratory with the help of RNA-sequencing. The causal mutations have been successfully identified for two of them and the target genes are located in the pericentromeric regions. Detailed information on the mapping-by-sequencing, mapping-and-sequencing, and RNA-sequencing assisted linkage mapping are presented and some limitations and challenges on the mapping assisted by RNA sequencing are also discussed. The alternative and elegant methods presented in this review may greatly accelerate forward genetics of barley mapping, especially for laboratories without large funding.     

对青藏东北缘现今块体划分、运动及变形的初步研究

利用2维非连续变形分析方法（DDA），以位移代替围压作为边界约束力，研究青藏东北缘现今块体划分及其运动变形。根据该地区地质构造及地震活动，以GPS点测量位移作为模拟结果约束点，得出了较合理的块体划分模型和随时间演化的主应变分布图，并把应变高值区与近几年来发生的5级以上地震作对比，得出了研究区内地震危险性可能较大的区域。另外，对模拟的甘青块体与阿拉善块体的边缘带断裂左旋运动做了大概计算。     

Discovery of 13 New Variable Stars in the Field of the Open Cluster NGC 2168 （M35）

A wide-field time-series CCD photometric survey of variable stars in the field of the open cluster NGC 2168 was carried out using the BATC Schmidt telescope. In total 13 new variable stars are discovered with three W UMa systems, one EA type and two EB type eclipsing binaries (one of them could be a W UMa system), and seven pulsating stars including three candidates of δScuti stars.     

Revisiting Sustainable Development of Dry Valleys in Hengduan Mountains Region

Dry valleys are a striking geographic landscape in Hengduan Mountains Region and are characterized by low rainfall, desert type of vegetation and fragile environment. Past efforts and resources have been concentrated mainly on rehabilitation of degraded ecosystem and fragile environment, particularly reforestation, while socio-economic development has been largely overlooked. Despite successes in pocket areas, the overall trend of unsustainability and environmental deterioration are continuing. It is important to understand that uplift of the Tibetan Plateau is the root cause of development of dry valleys, and development and formation of dry valleys is a natural process. Human intervention has played a secondary role in development of dry valleys and degradation of dry valleys though human intervention in many cases has speeded up environmental degradation of the dry valleys. It is important to understand that dry valleys are climatic enclaves and an integrated approach that combines rehabilitation of degraded ecosystems and socio-economic development should be adopted if the overall goal of sustainable development of dry valleys is to be achieved. Promotion of niche-based cash crops, rural energy including hydropower, solar energy, biogas and fuelwood plantation is recommended as the priority activities.     

Cytoplasmic regionalization in starfish oocyte occurrence and localization of cytoplasmic determinants responsible for the formation of archenteron and primary mesenchyme in starfish (asterias amurensis) oocytes

Starfish oocytes with intact germinal vesicles (GVs) were cut along desired planes with glass needles or ligated using silk thread loops into two parts and allowed to mature in vitro, and inseminated. The experimental results showed that (1) only the parts with GVs or partial GV contents (PGVCs) cleaved, those without any GV materials did not; but nucleated and non-nucleated fragments cut from mature eggs were able to divide; (2) the development of animal parts of oocytes containing GVs or PGVCs was like that of animal fragments of matured oocytes with female pronuclei; most of them gave rise to permanent blastulae, and just a few formed ectodermal vesicles with a little primary mesenchyme; (3) a large part of vegetal fragments with GVs or PGVCs, and the vegetal parts of mature eggs without female pronuclei developed into small but normal embryos; (4) the fragments containing GVs or PGVCs obtained from the oocytes along a plane parallel to the animal-vegetal (A-V) axis developed as normally as the halves (with or without female pronuclei) severed from mature eggs along the same axis. Based on the data above, it was concluded that (1) the non-chromatin materials in the oocyte GVs are indispensable for successful fertilization and cleavage of starfish eggs; (2) some factor (s) located asymmetrically in the vegetal hemispheres of starfish oocytes is (are) responsible for formation of the archenteron and primary mesenchyme. It is evident from the above findings that the oocyte cytoplasm of the starfish had already regionalized before the GV break-down. Contribution No. 1722 from the Institute of Oceanology, Academia Sinica     

Evolution of Electric Currents Associated with Two M-Class Flares

Using one-minute cadence vector magnetograms from Big Bear Solar Observatory (BBSO), we analyze the temporal behavior of derived longitudinal electric currents associated with two flares on July 26, 2002. One of the events is an M1.0 flare which occurred in active region NOAA 10044, while the other is an M8.7 flare in the adjacent region 10039. Rapid changes of magnetic fields in the form of flux emergence are found to be associated with both of these events. However, the temporal behavior of electric currents are very different. For the M1.0 flare, the longitudinal electric current density drops rapidly near the flaring neutral line; while for the M8.7 flare, the current density rapidly increases, confirming the picture of the current-carrying flux emergence. We offer a possible explanation for such a difference: magnetic reconnection at different heights for the two events, near the photosphere for the M1.0 flare, and higher up for the M8.7 flare.     

The formation and stability of the configuration of the planetary system HD 126611

Recent Doppler velocity measurements have revealed the existence of two planets orbiting the star HD 12661 on medium-eccentricity orbits. The inner planet has a period of 263.6 d and a mass of 2.3 Jupiter masses, and the outer planet has a period of 1444.5d and a mass of 1.57 Jupiter masses. The stability of this system requires the two planets to be in a state of mean-motion orbit resonances. By numerical method we have studied the orbit migration and stability of the system in its early ages under the action of the proto-stellar disk, and calculated the probabilities of the planets being captured into the mean -motion resonances during their migrations. It is found that at present the two planets are probably situated at the edge of the 11:2 mean-motion resonance and are in chaotic motions. This result may be helpful to clarify the arguments on the present configuration. Besides, it is indicated that very probably, after the formation of the system, the gaseous disk has almost disappeared before the planets migrated to the present configuration.     

浙西北江山─绍兴断裂带构造演化特征

江山—绍兴断裂带是中国南方最重要的构造带之一。它是江南陆块与华夏陆块神功运动形成的碰撞拼贴带。它表现出明显的多期变形、构造叠加的特征：神功运动形成近东西向平卧褶皱；印支运动沿断裂带有向北西强烈的推覆挤压。断裂带不同时期的活动反映了不同特征的构造环境，形成不同的构造变形特征，并对两侧的地质历史具有明显的控制作用。     

A Time Evolution Study of the Superhumps of the Dwarf Nova 1RXS J232953.9+062814 by Wavelet Transform

The time evolution behaviour of the superhumps of the dwarf nova1RXS J232953.9+ 062814 is investigated with the wavelet analysis method. On the basis of two nights CCD photometry performed during its first superoutburst as well as other published brightness data, we reveal the superhump's time-dependence as a function of periods and time. Our light curves, which phased in the rapid decay ending portion of the superoutburst and in the dawn of a following normal outburst, are important to help trace the superhump evolution for the star. Both the superhump period and the orbital period of the binary system are detected in the present data. We obtain P _sh=0.0458±0.0002d and P _orb=0.0450±0.0002d. They agree with those existing values. The two periods exchanged their roles during the superhump evolution. The general profile of brightness fading over the outbursts roughly followed an exponential decay law or a form of a five-order polynomial. This revised version was published online in July 2006 with corrections to the Cover Date.