沉积岩有机质的红外光谱及其在石油有机地球化学中的初步应用

Types and degree of muturity of organic matters in sedimentary rocks as index for oil generation and evolution can be determined by infrared spectrometry. In normal cases,oil-generating rocks can be preliminarily identified by infrared spectra of solubable bitumen components. However, the spectra of condensed nuclei of aromatic hydrocarbon should be resorted to whenever the composition and structure of organic matters arc comparative complex and their degree ofmuturity are not all the same. Systematic researches of known samples show that a configuration of condensed aromatic hydrccarbon can be proposed on the basis of the index of aromatic hydrocarbon structure and the ratio of methyl and methylene to aromatic nucleus. It is evidenced by preliminary experience that oil- and coal-generating organisms can be distinguished from each other with the help of this configuration.     

应用同位素标记相对和绝对定量技术分析水蛭对IgA肾病血瘀证大鼠差异蛋白的表达

目的：运用同位素标记相对和绝对定量技术（iTRAQ）分析水蛭对IgA肾病血瘀证大鼠差异蛋白的表达，并从血清蛋白质水平阐释水蛭治疗IgA肾病血瘀证的作用机制。方法：选用6周龄的Wistar雄性大鼠80只，运用脂多糖牛血清白蛋白+脂多糖+四氯化碳方法建立实验性IgA肾病模型，造模成功后，随机分为模型对照组、强的松治疗对照组和水蛭治疗组，每组各20只，另取20只为空白对照组，给药喂养至14周末后进行采血，血液样本经过SDS-PAGE电泳后进行iTRAQ标记，运用离线二维液相色谱分离与点靶以及质谱分析及数据库检索对各组样本进行相对定量分析，并利用生物信息学软件进行分析，以上实验再重复进行2次。结果：取4组均有表达且差异倍数≥1.2或≤0.83为筛选标准的蛋白纳入数据分析，经水蛭治疗组治疗后下调的差异蛋白共21个，这些差异蛋白主要参与低密度脂蛋白颗粒受体分解代谢、脂肪酸体内平衡、胆固醇稳态以及线粒体分裂等生物过程，其差异可能与补体和凝血级联通路、代谢信号通路、嘌呤代谢和嘧啶代谢途径等有关。结论：应用iTRAQ标记方法可筛选出水蛭治疗IgA肾病血瘀证大鼠的差异蛋白表达，并能揭示水蛭治疗IgA肾病血瘀证的物质基础及活血祛瘀的疗效机制。