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毛蚶血清凝集素的凝集活性 总被引:2,自引:1,他引:2
以毛蚶为实验材料,对其血清中的凝集素进行研究.毛蚶血清凝集素对人的A,B,O血型红细胞、鸡和小鼠的红细胞均有凝集作用,凝集效价分别为256,256,8,16,64,同时它还能对白色念珠菌、啤酒酵母、溶藻胶弧菌等微生物具有凝集作用.毛蚶血清凝集素具有一定的pH值稳定性和热稳定性.在选用的12种单糖和寡糖中,L-鼠李糖、L-树胶醛糖、麦芽糖、D-半乳糖、蔗糖对毛蚶血清的凝集活性有明显的抑制作用.用弧菌对毛蚶进行免疫刺激时,其血清对鸡红细胞的凝集效价明显增加. 相似文献
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Information regarding antioxidant enzymes in amphioxus remains lacking, and this study was carried out to examine the activities of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPX) in different genders and tissues of amphioxus Branchiostoma belcheri tsingtauense. Results show that (1) CuZn-SOD, CAT and GPX activities in the whole amphioxus B. belcheri tsing- tauense were basically at the same levels in male and female amphioxus, whereas both T-SOD and Mn-SOD activities in male amphioxus were significantly higher than that in the female (P<0.05); (2) The testis had significantly higher T-SOD and CuZn-SOD activities than the ovary (P<0.05); (3) CuZn-SOD activity was undetectable in the guts of male and female amphioxus; (4) For both male and female am- phioxus, the activities of CAT and GPX in the gonads including testis and ovary were the lowest (P<0.05) among the tissues examined; (5) The gut and gill had the same level GPX activities while the gut had a higher CAT activity; (6) There was no clear difference in CAT and GPX activities in the corresponding tissues between male and female amphioxus. The study on SOD, CAT and GPX activities in different genders and tissues of the protochordate provides data for future comparison of amphioxus antioxidant enzymes with those of invertebrates and vertebrates. 相似文献
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盐度对牙鲆非特异性免疫功能的影响 总被引:6,自引:0,他引:6
实验以健康牙鲆成鱼为研究对象,分别饲养于盐度为40,30(对照组),20的海水中,以牙鲆血清的凝血活性、溶菌酶含量以及补体因子C3含量为指标,研究盐度对牙鲆非特异性免疫功能的影响。结果表明,盐度增加或降低3d后血清凝血活性升高4倍,并一直保持至12d不变;溶菌酶含量先是升高,在第5天达到高峰后又逐渐降低,至第12天的含量反而略低于对照组;C3的含量在第一天取样时就比对照组有了很大的增长,随后逐渐下降,在第7天后恢复正常。盐度变化导致牙鲆非特异性免疫因子变化,说明在养殖生产中维持稳定环境极为重要。 相似文献
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