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黑鲷嗅上皮的超微结构 总被引:8,自引:0,他引:8
通过扫描与透射电镜观察了黑鲷嗅上皮的超微结构,嗅上皮内侧是感觉上皮区,其主要的细胞类型是:纤毛感觉细胞,微绒毛感沉细胞,柱状细胞,支持细胞和基细胞,非感觉上皮分布在嗅上皮边缘及点缀于感觉上皮区,似指状结构,具有粘液细胞,支持细胞,基细胞等,根据上述观察结果,可认为黑鲷属以视觉为主进行活动的鱼类。 相似文献
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本研究首先从施氏鲟(Acipenser schrenckii)性腺转录组中获得nanos1(Asnanos1)基因全长c DNA,并对其序列的准确性和特征分别进行PCR验证和生物信息学分析。进一步利用荧光定量RT-PCR技术检测Asnanos1基因在雌、雄施氏鲟的性腺、心脏、鳃、脑、肾、肝脏、脾脏等组织中的表达量。利用荧光定量RT-PCR技术检测Asnanos1基因在雌、雄施氏鲟的性腺、心脏、鳃、脑、肾、肝脏、脾脏等组织中的表达量。结果显示:Asnanos1的c DNA全长为1 389 bp,其中,5′非编码区(UTR)为414 bp,3′UTR为279 bp,ORF为696 bp。该基因共编码231个氨基酸。Asnanos1基因在施氏鲟除心脏以外的各组织中均有表达,在雌、雄鱼的鳃、脑、肾、肝脏、脾脏等组织中表达量无显著差异,但在卵巢中的表达量显著高于其他各组织(P0.05)。所得到的结果可为人工培育全雌施氏鲟苗种提供一些基础资料。 相似文献
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扫描电镜和透射电镜研究显示:牙鲆Pardlichthys olivaceus嗅上 皮的边缘为非感觉区,内侧为连续的感觉区,属嗅觉鱼类。非感觉区由表皮细胞、粘液细胞及基底细胞等组成,表皮细胞在游离面相互连接形成似指纹状的的结构,感觉区由纤毛毛感觉细胞、毛感觉细胞,纤毛非感觉细胞、支持细胞及基底细胞等组成。 相似文献
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为了解中华蛸(Octopus sinensis)受精卵的孵化及幼体发育机制,本研究对其章鱼胺受体基因(OsOARβ2R)进行克隆和生物信息学分析,同时研究了OsOARβ2R在不同组织/器官、刚孵出的幼体不同饥饿时间及不同的胚胎发育时期表达水平的变化。结果为:OsOARβ2R开放阅读框全长为1 158 bp,编码385个氨基酸,有7个跨膜结构域,具有G蛋白偶联受体的共性,其中TM3、TM5、TM6、TM7 4个跨膜结构相对保守。经氨基酸同源对比及构建系统进化树分析,其与加州双斑蛸(O.bimaculoides)的OAR的一致性最高。荧光定量PCR结果显示,在成体10个组织/器官中,OsOARβ2R在后唾液腺中表达水平最高,其次是脑和小肠;在幼体饥饿实验中,随着饥饿时间的推移,OsOARβ2R的表达水平在饥饿2 d后显著降低,在饥饿3 d时表达水平显著升高,且表达水平达到最高,之后表达水平开始回落;OsOARβ2R在中华蛸整个胚胎发育周期均可检测到,且在多细胞期表达水平最高,后显著下降,黑珠期显著高于红珠期及初孵幼体。这些结果为研究中华蛸受精卵的孵化及幼体发育机制、提高人工育苗效率提供了基础资料。 相似文献
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对厦门海区的纹藤壶 Balanusamphitriteamphitrite和鳞笠藤壶 Tetraclitasquamosasquamosa 雄性生殖系统进行解剖学、组织学和细胞化学的研究。结果表明:两种藤壶的雄性生殖系统均由精巢、输精管、贮精囊及交接器4部分组成。精巢内依次排列有精原细胞、精母细胞、精子细胞和成熟精子。输精管由一层上皮细胞围成,内可见成熟精子,贮精囊前端管壁结构与输精管相似,后端管壁厚,可分为4层结构。交接器结构复杂,内层为射精管,外层结构与贮精囊后段相似。精子为线形,头部附属小滴内有4—6个致密斑,鞭毛沿头部一侧着生。初步探讨了不同发育阶段的雄性生殖细胞碱性蛋白的变化及附属小滴的细胞化学特性及生理学特性 相似文献
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The family Sciaenidae is remarkable for its species richness and economic importance. However, the cytogenetic data available in this fish group are still limited, especially those obtained using fl uorescence in situ hybridization(FISH). In the present study, the chromosome characteristics of a sciaenid species, Argyrosomus amoyensis, were examined with several cytogenetic methods, including dual-FISH with 18 S and 5 S rDNA probes, and a self-genomic in situ hybridization procedure(Self-GISH). The karyotype of A. amoyensis comprised 2 n=48 acrocentric chromosomes. A single pair of nucleolar organizer regions(NORs) was located at the proximal position of chromosome 1, which was positive for silver nitrate impregnation(AgNO_3) staining and denaturation-propidium iodide(DPI) staining but negative for Giemsa staining and 4',6-diamidino-2-phenylindole(DAPI) staining, and was confi rmed by FISH with 18 S rDNA probes. The 5 S rDNA sites were located at the centromeric region of chromosome 3. Telomeric FISH signals were detected at all chromosome ends with dif ferent intensities, but internal telomeric sequences(ITSs) were not found. Self-GISH resulted in strong signals distributed at the centromeric regions of all chromosomes. C-banding revealed not only centromeric heterochromatin, but also heterochromatin that located on NORs, in interstitial and distal telomeric regions of specifi c chromosomes. These results suggest that the karyotype of Amoy croaker was relatively conserved and primitive. By comparison with the reported cytogenetic data of other sciaenids, it can be deduced that although the karyotypic macrostructure and chromosomal localization of 18 S rDNA are conserved, the distribution of 5 S rDNA varies dynamically among sciaenid species. Thus, the 5 S rDNA sites may have different evolutionary dynamics in relation to other chromosomal regions, and have the potential to be ef fective cytotaxonomic markers in Sciaenidae. 相似文献