鳗弧菌(Vibrio anguillarum)M3菌株生长条件及其对蛋白酶产量的影响

采用体外测定细菌浓度、胞外产物(ECP)蛋白含量和蛋白酶活力的方法，进行了鳗弧菌M3菌株在2216E培养基中的培养条件研究。结果表明，该菌株用固体培养基培养至24h左右，可得到较高的菌体浓度、ECP蛋白含量和蛋白酶活力。采用响应面分析方法设计实验，用SAS统计软件分析数据，得到NaCl浓度、pH值和温度对菌体生长及蛋白酶产量影响的回归模型。在2216E培养基的基础上，添加不同氮源、碳源物质以及不同浓度蛋白胨进行生长研究。结果表明，胰大豆蛋白胨能促进菌体生长及ECP蛋白分泌；NH4Cl与酪蛋白水解物可抑制蛋白酶的产生；牙鲆肌肉匀浆对菌体、ECP蛋白产量和蛋白酶产生有不同程度的促进作用；培养基中蛋白胨浓度为4％时菌体量与ECP蛋白含量达最高值，在蛋白胨浓度为2％时蛋白酶分泌量已稳定；1％的葡萄糖、蔗糖、甘油均能显著地提高菌体及ECP蛋白产量，却抑制了蛋白酶的产生。     

A novel method for evaluating proneness of gas outburst based on gas-geological complexity

The gas outburst, resulting in substantial economic losses and even casualties, is the biggest obstacle in coal mines, mostly caused by an imbalance of gas-geological structure. For accurately measuring this proneness, in this paper, a novel evaluation method was proposed based on the gas-geology theory. In this method, a standardization model of statistical units was presented first, which was used to standardize and quantify the 12 chosen gas-geological factors; and then, an associated function was established for computing the gas-geological complexity index (GCI). With increasing GCI values, the evaluated area was divided into four grades: simple, medium, complex, and extremely complex region, in which the associated proneness of outbursts was SAFE, POTENTIAL, HIGH, STRONG, respectively. Taking the XueHu Coal Mine as an example, site verification was carried out with a good result. Research and application indicate that (1) gas outburst is unbalanced and closely related to the complex of the gas geological structure, showing a greater GCI leads to a higher outburst possibility; (2) the most likely area for the gas outburst is the extremely complex region and the transition zone between adjacent areas with different GCI grades; (3) upgrading-targeted control measures are the best way for preventing and controlling disasters caused by the gas and outburst unbalanced distribution. This novel method provided a reliable quantity approach for predicting and zonally managing gas outbursts and improving the effectiveness of outbursts prevention.

     

草鱼、鲢鱼和凡纳滨对虾多元化养殖系统结构优化的研究

采用陆基围隔实验法,开展了草鱼(Ctenopharyngodon idellus)、鲢鱼(Hypophthalmichthys molitrix)和凡纳滨对虾(Litopenaeus vannamei)三元混养结构优化的初步研究。实验于2009年5～10月进行,每月定期采样,分别测定了各混养系统水质和底质的变化、养殖生物的成活率和养殖产量,分析了各混养系统的饲料转化效率、氮磷利用率及产出投入比。实验结果初步表明,草鱼放养密度为0.77尾/m2时,既可以保证出池规格(>1 100 g/尾)又不影响收获产量(>8 400 kg/hm2),同时饲料转化效率(>52%)及氮磷利用率(N>30%,P>14%)也较高;鲢鱼放养密度为0.23尾/m2或0.45尾/m2,可以起到调节水质的作用;对虾放养密度越大(48.9尾/m2),底质被污染程度越小。在本实验条件下,最佳的混养模式:草鱼与鲢鱼混养比例为草鱼0.77尾/m2鲢鱼0.45尾/m2;草鱼、鲢鱼和对虾混养比例为草鱼0.77尾/m2鲢鱼0.23尾/m2凡纳滨对虾16.3尾/m2。     

养殖牙鲆鱼苗腹水症病原菌的鉴定及系统发育学分析

从患腹水症养殖牙鲆鱼苗分离到一株细菌B17，人工感染实验证实春具有致病性。用Biolog-GN细菌鉴定系统对B17进行测试，发现其与参考菌株的相似性较低，不能进行鉴定。常规生化测试表明，B17具有弧菌属的特征，其表型特征与Vibrio splendidus非常相似。为了进一步明确菌株B17的分类学地位，测定了其16SrRNA基因序列，分析了相关细菌相应序列的同源性，构建了系统发生树，结果表明菌株B17与V. splendidus的亲缘关系最近。综合上述结果，菌株B17可鉴定为V.splendidus。     

草鱼不同养殖模式底泥间隙水与上覆水营养盐的分布特征

于2009年6～10月采集草鱼(Ctenopharyngodon idellus)不同养殖模式围隔底泥的样品,对上覆水和间隙水中营养盐形态与含量进行分析,探讨间隙水和上覆水中营养盐浓度间的相关关系。结果表明:草鱼不同养殖模式围隔大部分间隙水中营养盐浓度要高于上覆水,上覆水与间隙水中PO43--P浓度呈极显著的正相关性(P<0.01)。上覆水和间隙水中的NO2--N和NO3--N浓度垂直分布特征随月变化规律较一致。养殖期间,间隙水溶解态无机氮(DIN)中NH4+-N比例最高,占95%以上。间隙水中NH4+-N浓度随深度增加而逐渐升高(0～10cm),而NO2--N和NO3--N浓度则随深度增加而减小(0～4cm)。表层底泥在0→1cm方向PO43--P浓度明显增加,从2～3cm层后多数养殖模式下浓度缓慢下降,而4→10cm方向上浓度会随深度增加而明显下降。总体而言,三元混养组中(GSL2)上覆水中营养盐的浓度一般要低于GSL1组和GSL3组,且养殖期间该组底层水DO浓度要高于GSL1组和GSL3组,初步认为是一种较为优化的草鱼混养模式。     

庐枞盆地浅表地壳速度成像与隐伏矿靶区预测

利用反射地震初至波可以精确反演地壳速度结构和构造信息.对庐枞盆地采集的高分辨地震数据,运用初至波层析成像方法,反演得到了该区1200 m以上的浅表地壳速度结构信息.找矿信息总是同地球化学异常联系紧密,对采集于炮孔深处的岩屑、泥砂样品进行了地球化学分析,发现了多处显著的金属元素异常.对五条测线的速度成像结果及其映射的地下岩性结构和炮孔地球化学分析结果进行了相关性对比分析.结果显示,庐枞盆地的沉积岩、火山岩和侵入岩具有不同的速度范围,侵入岩具有高波速特征,地球化学信息高异常往往出现在高速侵入岩体的上方;精细的速度信息蕴含着丰富的浅表地壳结构变化特征,与庐枞矿集区已知的地质、岩体、构造和矿体分布存在着良好的对应关系.速度成像结果可以准确刻画地下隐伏侵入岩体的空间分布形态,结合地球化学分析异常和重磁探测等信息,预测隐伏矿床,提供深部找矿靶区.     

Analysis of microscopic pore structures of the silty clay before and after freezing–thawing under the subway vibration loading

With the rapid development of the subway rail transit, the effect of the cyclic loading on the surrounding foundations and buildings has drawn wide attention. In addition to the in situ tests and the laboratory triaxial tests, microscopic tests also provide an effective way to clarify the physical and mechanical characteristics of soils. On the other hand, the characteristics of the soft silty clay before and after freezing–thawing has been less studied. In this paper, the scanning electron microscope (SEM) tests following the cyclic triaxial tests of silty clay layer were performed to investigate the variations of the microscopic pore structures of the layer before and after freezing–thawing. The corrected Otsu method was used to obtain the binary SEM images of silty clay. The porosity results demonstrate that the magnifications from 1000× up to 5000× were suitable for observation of the silty clay microstructures. The binary SEM images of soil pore structures were quantitatively analyzed, including the porosity, the size distribution, the pore shape coefficient, the pore orientation distribution and the fractal dimension. The pore orientation of samples without loading is arranged in the horizontal direction, while the pores of samples under cyclic loadings are arranged in the vertical. After freezing–thawing, the mean anisotropy value of the microscopic pore structures increased about 12% and the porosity of samples without loadings increases about 11.24%. The lower the freezing temperature is, the larger the porosity within the samples becomes. However, the freezing–thawing has little effect on the pore shape coefficient of the silty clay. The porosity of the silty clay increases with an increase in pore diameter, but it decreases with the increase in excess pore pressure. In addition, the microscopic pore structures of the silty clay exhibit fractal characteristics. The fractal dimension is reduced by the disturbance from the effect of freezing–thawing, coupled with the effect of cyclic loading.     

结晶岩地区深地震数据采集关键技术与方法

深反射地震是了解深部地质结构的主要手段,获取高品质的数据是给出合理地质解释的基础.在结晶岩地区,由于地层成层性差、非均质性严重,地震散射效应明显,导致地震波场复杂,同时结晶岩为非理想弹性体,不利于地震波能量转换,有效反射能量弱,信噪比低.加之深反射地震的目标层较深且受环境噪声干扰严重,高频信号衰减快,地震资料主频较低.这些因素使得在结晶岩区难以获得高品质的地震资料,为探索提高该类地区深反射地震资料质量的方法和技术,本文依托长江中下游成矿带2009—2014年深反射地震数据采集工作,在精细设计、严格施工的基础上,从激发和接收入手,开展了"轴向不耦合激发"、"宽频接收"和"宽线观测"等技术方法试验研究.结果表明,这些方法技术措施提高了下传弹性波能量,展宽了接收地震信号的频带,提高了覆盖次数和信噪比,有效改善了地震原始资料的品质和成像效果.研究结果对今后结晶岩地区深地震反射数据采集工作具有重要的实用价值和参考意义.