High matrix interference affecting detection of PAH metabolites in bile of Atlantic hagfish (Myxine glutinosa) used for biomonitoring of deep-water oil production

The characteristic biology and wide distribution of hagfish species makes them relevant for use in pollution biomonitoring at great water depths, particularly in regions where deep-water oil production may take place. The exposure of fish to petrogenic contaminants can normally be detected from the level of polycyclic aromatic hydrocarbon (PAH) metabolites in bile fluid. Some of these metabolites are strong fluorophores, allowing analytical detection by means of simple fluorometric techniques such as fixed wavelength fluorescence (FF) and synchronous fluorescence scanning (SFS). In the present study bile from Atlantic hagfish (Myxine glutinosa) collected in pristine areas (Barents Sea and southwestern Norway) displayed strong bile fluorescence levels, suggesting the presence of PAH contaminants. However, gas-chromatography-mass spectrometry (GC-MS) analyses ruled out PAHs as the origin for this fluorescence signal. Rather, the bile of Myxine contains components resulting in unusually strong background fluorescence interfering at the wavelength pairs used for detection of PAH metabolites. Possible background for the observed matrix interference and implications for detection of PAH metabolites in hagfish is discussed.     

Acidic compounds in biodegraded petroleum

Twelve oil samples have been characterised by titration, FT-IR and chromatographic analysis to determine the differences between the organic acid composition of biodegraded and non-biodegraded oils. The biodegraded oils have higher total acid and total base contents, both by titration and extraction. The molecular weight ranges of the extracted acids are lowest in the biodegraded oils, and the equivalent weight calculations indicate a dominance of multi-functional molecules. Gel permeation chromatography gives a molecular weight range with most of the molecules between 300 and 500 g/mol. FT-IR shows that the extracted acids from biodegraded oils are more carboxylic and aliphatic while the non-degraded oils are more phenolic. Molecular analysis of the derivatised extracts give UCM envelopes for biodegraded oils, and no molecular identification. The results indicate that the acidic constituents in biodegraded oils are a product of the biodegradation, as the composition is very different from the non-biodegraded oils.     

基于多期DEM和滑坡强度的滑坡风险评估

滑坡危险性定量评估是滑坡风险评估中的关键和难点,也是当前国际风险管理研究中的热点问题.以滑坡密集分布的黑方台南塬为研究区,以32处典型滑坡为研究对象,依据多期三维数字高程模型(DEM),提出了一种基于强度的滑坡危险性定量评估技术方法.根据多期三维地形信息的解译及野外调查,编制多期滑坡分布图,计算滑坡活动的频率.利用GIS技术,利用滑坡体积与速度的乘积计算滑坡强度.将滑坡危险性定义为滑坡频率和滑坡强度的乘积,同时调查和分析了黑方台地区各类承灾体的类型、价值及其在相应滑坡强度下的易损性,在此基础上开展了单体滑坡风险评估和黑方台南塬滑坡风险区划.     

Numerical methods for flow in a porous medium with internal boundaries

Flow of fluids and transport of solutes in porous media are subjects of wide interest in several fields of applications: reservoir engineering, subsurface hydrology, chemical engineering, etc. In this paper we will study two-phase flow in a model consisting of two different types of sediments. Here, the absolute permeability, the relative permeabilities and the capillary pressure are discontinuous functions in space. This leads to interior boundary value problems at the interface between the sediments. The saturation S_w will be discontinuous or experience large gradients at the interface. A new solution procedure for such problems will be presented. The method combines the modified method of characteristics with a weak formulation where the basis functions are discontinuous at the interior boundary. The modified method of characteristics will provide a good first approximation for the jump in the discontinuous basis functions, which leads to a fast converging iterative solution scheme for the complete problem. The method has been implemented in a two-dimensional simulator, and results from numerical experiments will be presented. This revised version was published online in August 2006 with corrections to the Cover Date.     

Offshore and shipping activities in the Norwegian Arctic areas: The environmental dimension: Case: Norsk Hydro''s drilling of well 7316/5-1, autumn 1992

This paper describes how Norsk Hydro planned and executed the safety, environment and emergency preparedness matters related to the exploration drilling of well 7316/5-1, the most northern well drilled on the Norwegian continental shelf. This well (1992) was Norsk Hydro's first experience with the new above-mentioned regulations. For later wells, Norsk Hydro developed both how to use and implement these new regulations.     

Modified adaptive local–global upscaling method for discontinuous permeability distribution

The paper is devoted to the upscaling method appropriate for single-phase flow in media with discontinuous permeability distribution. The suggested algorithm is a modification of the iterative adaptive local–global upscaling developed by Chen and coauthors. The key feature of this method is a consistency between local and coarse global calculated characteristics. In this work, we apply a modified procedure to determine the boundary conditions used in the local fine-scale computation. To increase the accuracy of these boundary conditions on each iteration, we involve an additional preliminary step based on the results of coarse scale calculations from the previous iteration. Numerical tests show an essential improvement of the accuracy of upscaled flow rates for most of the realizations of statistical permeability distribution. Although the developed method is universal, its efficiency increases with increasing of permeability contrast.     

Preparation of a Synthetic Titanite Glass Calibration Material for In Situ Microanalysis by Direct Fusion in Graphite Electrodes: A Preliminary Characterisation by EPMA and LA-ICP-MS

This paper describes a technique for the preparation of a titanite (CaTiSiO₅) glass calibration material for use in in situ microanalysis of major, minor, and trace elements in geological materials. The starting composition was a titanite matrix doped with minor and trace elements at ∼ 200 μg g^-1. The elements Sc, Y, REEs, Th and U were added in the form of nitrates in solution, and the elements V, Cr, Mn, Fe, Co, Ni, Zr, Nb, Hf and W were added as solid oxides. The synthetic titanite glass was produced by direct fusion by resistance heating in graphite electrodes at 1600-1700 °C, and quenched in air. Backscattered electron images indicate good homogeneity, with no signs of separate phases or vesicles, and analysis of the major elements Ca, Ti and Si by electron microprobe showed relative standard deviations between 0.5 and 0.7%, based on six independent measurements. Deviations from nominal concentrations for Ca, Si and Ti were measured to -1.2, -3.3 and -0.8%, respectively. The homogeneity of the trace elements in the glass was assessed by LA-ICP-MS analyses, using NIST SRM 610, 612 and 616 as external calibrators, and Ca as the internal standard element. Determinations were made both with a quadrupole mass spectrometer and a sector field instrument, and both raster and spot modes of analysis were used. For the majority of doped elements, precision was better than 10%, and relative deviations from nominal values were, with few exceptions, between 5 and 10%.     

Wave modelling - The state of the art

This paper is the product of the wave modelling community and it tries to make a picture of the present situation in this branch of science, exploring the previous and the most recent results and looking ahead towards the solution of the problems we presently face. Both theory and applications are considered.The many faces of the subject imply separate discussions. This is reflected into the single sections, seven of them, each dealing with a specific topic, the whole providing a broad and solid overview of the present state of the art. After an introduction framing the problem and the approach we followed, we deal in sequence with the following subjects: (Section) 2, generation by wind; 3, nonlinear interactions in deep water; 4, white-capping dissipation; 5, nonlinear interactions in shallow water; 6, dissipation at the sea bottom; 7, wave propagation; 8, numerics. The two final sections, 9 and 10, summarize the present situation from a general point of view and try to look at the future developments.