Effects of water-borne copper on metallothionein and lipid peroxidation in the marine amphipod Gammarus locusta

The aim of the current work was to determine over 10 days the effects of water-borne exposure of the marine amphipod Gammarus locusta to 4 microgCu l(-1) on the metallothionein (MT; measured by differential pulse polarography) protection system and lipid peroxidation (LP: thiobarbituric acid-reactive malondialdehyde equivalents) as a measure of oxidative damage. MT levels in exposed animals increased significantly at day 2 (36% > control; P < 0.001) and remained high at days 6 and 10 (55 and 38%, respectively, P < 0.001). The maximum level of MT at day 6 coincided with the highest Cu body-burden. LP increased within I day of exposure, indicative of Cu as an oxidative stressor. However, in contrast to MT, the highest LP level was seen at day 4 (68% > control, P < 0.001) before returning to control values by day 6, indicating a protective role of MT against the pro-oxidant effects of Cu.     

Microphytobenthos vertical migratory photoresponse as characterised by light-response curves of surface biomass

The migratory response of intertidal microphytobenthos to changes in irradiance was studied on undisturbed estuarine sediments. Two non-destructive optical techniques were used to trace variations in vivo of surface biomass: PAM fluorometry, for measuring the minimum fluorescence level (F_o); and spectral reflectance analysis, for quantifying the normalized difference vegetation index (NDVI). Following the formation of a dense biofilm at the surface, replicated sediment samples were simultaneously exposed to six different irradiance levels, ranging from 50 to 1500 μmol m⁻² s⁻¹, during a period of 120 min. The migratory photoresponse of the biofilms was characterised by constructing biomass vs. light curves (BLC), relating the accumulation of microalgal biomass after that period (estimated by F_o or NDVI) to the irradiance level incident on the surface. BLCs allow characterising the main features of the migratory photoresponse of intact biofilms. Typical BLC showed a clear biphasic pattern, with an increase in microalgal accumulation under irradiances below 100 μmol m⁻² s⁻¹, maximum values under 100–250 μmol m⁻² s⁻¹, and a gradual decrease of surface biomass under higher irradiances, indicating a strong photophobic downward migratory response. Similar BLC patterns were obtained when measuring F_o or NDVI. The construction of BLCs for biofilms from intertidal sites with distinctive sediment characteristics and diatom taxonomic composition allowed to detected significant differences in the migratory photoresponse. Biofilms from a muddy sediment exhibited considerably larger amplitude in the migratory photoresponse than the biofilms from a sandy mud site, especially under high irradiances. The photophobic migratory response to high light was found to vary among diatom species, particularly in the case of the biofilms from the muddy sediments.     

Multi-level assessment of chronic toxicity of estuarine sediments with the amphipod Gammarus locusta: II. Organism and population-level endpoints

This study aimed to test the performance of the amphipod Gammarus locusta (L.) in chronic sediment toxicity tests. It constitutes part of a multi-level assessment of chronic toxicity of estuarine sediments, integrating organism and population-level endpoints with biochemical markers responses. Here we account for organism and population-level effects, while biomarker responses were reported in a companion article. Five moderately contaminated sediments from Sado and Tagus estuaries were tested, comprising 3 muddy and 2 sandy sediments. These sediments either did not show acute toxicity or were diluted with control sediment as much as required to remove acute toxicity. Subsequent chronic tests consisted of 28-day exposures with survival, individual growth and reproductive traits as endpoints. Two of the muddy sediments induced higher growth rates in the amphipods, and improved reproductive traits. This was understood to be a consequence of the amount of organic matter in the sediment, which was nutritionally beneficial to the amphipods, while concurrently decreasing contaminant bioavailability. Biomarker responses did not reveal toxicant-induced stress in amphipods exposed to these sediments. One of the sandy sediments was acutely toxic at 50% dilution, but in contrast stimulated amphipod growth when diluted 75%. This was presumed to be an indication of a hormetic response. Finally the two remaining contaminated sediments showed pronounced chronic toxicity, affecting survival and reproduction. The sex ratio of survivors was highly biased towards females, and offspring production was severely impaired. The particulars of the responses of this amphipod were examined, as well as strengths versus limitations of the sediment test. This study illustrates the utility of this chronic test for toxicity assessment of contaminated estuarine sediments, with potential application all along Atlantic Europe.     

Interpolation by regularized spline with tension: I. Theory and implementation

Bivariate and trivariate functions for interpolation from scattered data are derived. They are constructed by explicit minimization of a general smoothness functional, and they include a tension parameter that controls the character of the interpolation function (e.g., for bivariate case the surface can be tuned from a membrane to a thin steel plate), Tension can be applied also in a chosen direction, for modeling of phenomena with a simple type of anisotropy. The functions have regular derivatives of all orders everywhere. This makes them suitable for analysis of surface geometry and for direct application in models where derivatives are necessary. For processing of large datasets (thousands of data points), which are now common in geosciences, a segmentation algorithm with a flexible size of overlapping neighborhood is presented. Simple examples demonstrating flexibility and accuracy of the functions are presented.On leave from the Department of Physical Geography and Cartography, Comenius University, Mlynská dolina, Bratislava, Czechoslovakia.On leave from the Institute of Physics, Dúbravská cesta 9, Bratislava, Czechoslovakia.     

A traveltime reciprocity discrepancy in the Podvin & Lecomte time3d finite difference algorithm

We have found that the extensively used finite difference scheme time3d produces time fields which are 'asymmetric' in the sense that traveltimes computed to the right of the source are faster than traveltimes computed to the left. All finite difference schemes will, as they are approximations to the wave equation, to some extent fail to obey reciprocity perfectly. We show, however, that the errors in time3d may be significant—and unnecessarily large. An asymmetry in the point source initialization has been identified, and after correction time3d produces time fields with an improved reciprocity.     

Geospatial analysis of a coastal sand dune field evolution: Jockey's Ridge, North Carolina

Preservation and effective management of highly dynamic coastal features located in areas under development pressures requires in-depth understanding of their evolution. Modern geospatial technologies such as lidar, real time kinematic GPS, and three-dimensional GIS provide tools for efficient acquisition of high resolution data, geospatial analysis, feature extraction, and quantification of change. These techniques were applied to the Jockey's Ridge, North Carolina, the largest active dune field on the east coast of the United States, with the goal to quantify its deflation and rapid horizontal migration. Digitized contours, photogrammetric, lidar and GPS point data were used to compute a multitemporal elevation model of the dune field capturing its evolution for the period of 1974– 2004. In addition, peak elevation data were available for 1915 and 1953. Analysis revealed possible rapid growth of the dune complex between 1915–1953, followed by a slower rate of deflation that continues today. The main dune peak grew from 20.1 m in 1915 to 41.8 m in 1953 and has since eroded to 21.9 m in 2004. Two of the smaller peaks within the dune complex have recently gained elevation, approaching the current height of the main dune. Steady annual rate of main peak elevation loss since 1953 suggests that increase in the number of visitors after the park was established in 1974 had little effect on the rate of dune deflation. Horizontal dune migration of 3–6 m/yr in southerly direction has carried the sand out of the park boundaries and threatened several houses. As a result, the south dune section was removed and the sand was placed at the northern end of the park to serve as a potential source. Sand fencing has been an effective management strategy for both slowing the dune migration and forcing growth in dune elevation. Understanding the causes of the current movements can point to potential solutions and suggest new perspectives on management of the dune as a tourist attraction and as a recreation site, while preserving its unique geomorphic character and dynamic behavior.