Enhanced a novel β-agarase production in recombinant Escherichia coli BL21(DE3) through induction mode optimization and glycerol feeding strategy

Agarases are hydrolytic enzymes that act on the hydrolysis of agar and have a broad range of applications in food,cosmetics and pharmaceutical industries. In this study, a glycerol feeding strategy based on induction mode optimization for high cell density and β-agarase production was established, which could effectively control acetate yield. First, exponential feeding strategy of glycerol with different overall specific growth rates(μ) was applied in the pre-induction phase. The results showed that the low μ(μ=0.2) was suggested to be the optimal for cell growth and β-agarase production. Second, the effects of induction temperature and the inducer concentration on cell growth and β-agarase production were investigated in the post-induction phase. When induced by isopropyl-β-d-thiogalactoside(IPTG), the strategy of 0.8 mmol/L IPTG induction at 20℃ was found to be optimal for β-agarase production. When cultivation was induced by continuous lactose feeding strategy of 1.0 g/(L·h), the β-agarase activity reached 112.5 U/mL, which represented the highest β-agarase production to date.Furthermore, the β-agarase was capable of degrading G. lemaneiformis powder directly to produce neoagarooligosaccharide, and the hydrolysates were neoagarotetraose(NA4) and neoagarohexaose(NA6). The overall research may be useful for the industrial production and application of β-agarase.     

南山站对陆地—5号资源卫星的可观测范围及以该站为中心的覆盖区域

本文简要介绍了陆地系列地球资源卫星的轨道特征，并以陆地—５号资源卫星为例，根据南山站的座标计算出该站对陆地—５号资源卫星的可观测范围及以该站为中心的卫星的覆盖区域。     

Magnetic stratigraphy observed in ferromanganese crust

Summary. A sample of ferromanganese crust deposit (SCHW-1D) was cut into two sets of 1 mm slices for palaeomagnetic study. Alternating field and thermal demagnetization, and isothermal remanent magnetization analyses were performed on each thin slice. The results reveal the presence of a stable magnetism and both normal and reversed polarity intervals in the specimens. The observed polarity intervals cannot be confidently correlated with the geomagnetic polarity time-scale of the last 10 Myr due to the polarity overlap inherent in the sampling technique. But the results confirm the slow accretion rate of ferromanganese deposit determined by ¹⁰Be method and suggest potential use of ferromanganese deposits in palaeomagnetic and tectonic studies.     

南海大鹏湾夜光藻种群在其生态群落中的地位和功能

分析了１９９１年上半年南海大鹏湾盐田水域夜光菏和浮游动、植物之间的种群动态关系，结果表明，在春季高峰期３月至５月初，调查海域共出现１２个夜光藻和浮游动、植物高峰。其中，以发生４次赤潮的夜光藻种群占主导地位，其次是枝角类的鸟啄尖头蚤，其最高数量高达９６２３８ｉｎｄ／ｍ＾３。相比之下，在夜光藻和枝角类岛啄尖头捕食压力的影响下，浮游植物的春季高峰并不显著。调查结果表明夜光藻的过度繁殖，不但对产浮游性卵的     

大鹏湾夜光藻种群的季节变化和分布特征

于１９９０年３月－１９９２年６月对中国南海大鹏湾盐田附近海域的夜光藻种群动态及时空分布进行了综合调查，结果表明，夜光藻种群的出现期间一般为每年的１－６月，最早是１９９１年的１２月，娄量高峰期为３－５月，种群密度的波动极为显著；调查期间共发生８次赤潮，且都发生在３月初至５月初；大鹏湾夜光藻赤潮的发生特点是，赤潮的发生和消失突然，持续时间短，一般不超过３ｄ。     

SNT地区氡法勘查砂岩型铀矿的应用效果

为了减小我国北方隐盲型砂岩铀矿找矿中钻孔布设的盲目性,实现快速评价,在二连盆地SNT地区开展了土壤氡法寻找砂岩型铀矿工作。经测量数据的统计处理,在测区内固定了有一定规模的氡异常晕6个。通过氡异常评价并结合该区地质、地球物理和地球化学条件,预测了3个远景段,经钻探在其中的一个远景段中发现了砂岩型铀矿化,表明在我国北方中一新生代盆地中开展土壤氡法寻找隐盲型砂岩铀矿是有效且可行的。     

城区水文循环机制与改善策略分析

在城区化过程中,由于人口快速成长且集中,随之而来的建筑物、道路等不透水面密度增加,导致地下水补给减少及破坏水文循环。为了解土地利用方式改变对长期水文量影响,建立一个适宜的城区水循环模式是非常重要的。选定台湾台北的六馆抽水站集水区进行分析,模式参数经过敏感度分析、率定与验证。采用改变不同透水铺面设置比例的方案,评估其对径流量、入渗量、蒸发散量的改变。并分析设置不同比例屋顶雨水贮集系统对地表径流量及供水量的改变。所建立的城区水循环模式是评估土地利用改变造成水文量的改变的一个有效工具,并可提供决策者做为城区土地利用决策的参考。     

^40Ar／^39Ar Fast Neutron Activation Ages of Quartz from the Jinman Vein Copper Dposit in Western Yunnan and Their Significance

Quartz samples collected from the Jinman vein copper deposit in the Lanping Basin of western Yunnan were determined by⁴⁰Ar/³⁹Ar fast neutron activation techniques, and the spectra are characterized as being saddle-shaped. The samples yielded a plateau age of 58.05 ± 0.54 Ma, a minimum appearance age of 56.76 ±0.81 Ma and an isochron age of 54.30 ± 0.15 Ma, the three ages being close to each other, indicating that the ages of the quartz samples so far determined are true and reliable. The plateau age represents the time of formation of Cu-bearing quartz veins, which is corresponding to Early Himalayan. This age is also consistent with the time at which a tectonically thermal event (60 Ma) took place within the Lanping Basin, Yunnan Province. In consideration of the fact that copper ore and other ore types in the vast area of western Yunnan are concentrated mainly in the Early Himalayan strata, the authors believe that there must have existed some indispensable key factors leading to metallogenesis on a large scale during the Early Himalayan period in western Yunnan and also constraining in union the formation of ore deposits there.     

A Laboratory Model for the Flow in Urban Street Canyons Induced by Bottom Heating

Water tank experiments are carried out to investigate the convection flow induced by bottom heating and the effects of the ambient wind on the flow in non-symmetrical urban street canyons based on the PIV (Particle Image Visualization) technique. Fluid experiments show that with calm ambient wind,the flows in the street canyon are completely driven by thermal force, and the convection can reach the upper atmosphere of the street canyon. Horizontal and vertical motions also appear above the roofs of the buildings. These are the conditions which favor the exchange of momentum and air mass between the street canyon and its environment. More than two vortices are induced by the convection, and the complex circulation pattern will vary with time in a wider street canyon. However， in a narrow street canyon， just one vortex appears. With a light ambient wind, the bottom heating and the associated convection result in just one main vortex. As the ambient wind speed increases, the vortex becomes more organized and its center shifts closer to the leeward building.     

三维重力内波和对流云街的启动机制

在一个下部为弱不稳定层、上部为稳定层的二层模式中，求解线性化的大气动力—热力学方程组，表明一个孤立山丘所引发的重力内波，在向下游传播过程中与边界层中对流相互作用，形成大气低层的涡漩活动，其图象与观测到的对流云街结构相似。