Regulation of Tilapia metallothionein gene expression by heavy metal ions

Tilapia is a common fish species inhabiting inland waters and estuarine regions in Hong Kong and Southeast Asia, and useful for bio-monitoring of metal pollution. Metallothionein (MT) gene expression in fish tissues has been useful to sub-lethal risk assessment as biomarker of exposure to metal ions in fishes inhabiting metal contaminated area. To investigate metal inductions of Tilapia MT gene expression in vivo, Tilapias were injected with different concentrations of heavy metals and tissues were then removed for quantitative PCR assay using mimic PCR methods. All of the metal ions tested (Cu(2+), Cd(2+), Hg(2+), Ni(2+), Pb(2+) and Zn(2+)) were able to induce hepatic MT mRNA levels. Renal MT mRNA levels of Cd(2+) and Zn(2+) treated fish was not induced with significant fold induction, however MT mRNA levels in gills were sensitive to the administrations of these metal ions. These data indicated that Tilapia MT mRNA levels in gills and liver are sensitive biomarker of exposure to various metal ions.     

Comparison of polycyclic aromatic hydrocarbon distributions and sedimentary organic matter characteristics in contaminated, coastal sediments from Pensacola Bay, Florida

In this study, we examined the distribution of polycyclic aromatic hydrocarbons (PAHs) in a contaminated coastal area and the characteristics of the natural organic matter in tandem. We present a detailed study of PAH concentration, distribution, and organic matter characteristics of three core samples from Pensacola Bay, Florida. Solid-state 13C Nuclear Magnetic Resonance (NMR), pyrolysis gas chromatography coupled with mass spectrometry (GC-MS), and tetramethyl ammonium hydroxide (TMAH) thermochemolysis GC-MS were applied to obtain structural details about the sedimentary organic matter. Elemental compositions (carbon and nitrogen) and estimates of black carbon contents are also reported. These coastal sediments were found to contain more PAHs in the upper 15 cm layers than in the bottom 15-25 cm samples. The samples that contained the most PAHs also contained the least amount of aromatic carbon and contained a significant amount of paraffinic carbon. Lignin-derived pyrolysis and TMAH thermochemolysis products were abundant and generally higher in all of the samples in comparison to those reported for modern coastal sediments, indicating a large flux of terrestrial carbon. The black carbon contents were found to range from 4.3% to 6.8%, which are significantly lower than other reports of black carbon in sediments, which represent as much as 65% of the total organic carbon content. The low black carbon content suggests that this type of refractory carbon may not be as responsible for regulating PAH distribution as indicated by other researchers.     

Inhibition of coral fertilisation and larval metamorphosis by tributyltin and copper

Fertilisation and larval metamorphosis of reef-building corals are important life history events leading to recruitment of juvenile corals to reef populations. Little is known of the sensitivity of these early life phases to pollution, or their relative susceptibility to certain toxicants compared with established coral colonies. Inhibition of fertilisation and larval metamorphosis of the coral Acropora millepora (Ehrenberg, 1834) was assessed in response to solutions of the antifoulants tributyltin (TBT) and copper (Cu) using laboratory-based bioassays. Nominal concentrations that inhibited 50% fertilisation and metamorphosis (IC50) were calculated from 4 h fertilisation and 24 h metamorphosis assays and were based on introduced dose. Cu was most potent towards fertilisation with an IC50 of 17.4 micrograms/l. TBT however, proved more toxic to larval metamorphosis having an IC50 of 2.0 micrograms/l. Inert surfaces coated with either Cu- or TBT-based antifouling paint also inhibited fertilisation and metamorphosis. The degree of inhibition was correlated with surface area of the paint coating. These results indicate fertilisation and metamorphosis of coral can be sensitive to active components of antifouling paints.     

基于Web搜索搭建OWS索引服务中心

OWS(OGC Web Service)为空间数据共享提供了可行的方法,但如何在浩瀚的互联网上快速、准确地查询到符合用户需要的OWS是一件比较困难的事情。在对Web搜索方法进行深入理解的基础上,提出一种基于全文检索搜索引擎搭建OWS索引服务的思路,并采用Google实现了OWS索引服务中心的原型系统,该系统可以为互联网用户提供OWS搜索服务。     

西藏雅鲁藏布江缝合带西段普兰纯橄岩中橄榄石的出溶特征及指示意义

西藏雅鲁藏布江缝合带西段普兰纯橄岩中橄榄石存在透辉石和磁铁矿共生出溶现象。这类纯橄岩非常新鲜,主要由橄榄石、尖晶石和透辉石等矿物组成。橄榄石的Fo值是90.1~90.7,铬尖晶石的Cr~#为19.8~20.8,远小于蛇绿岩地幔橄榄岩中常见纯橄岩中铬尖晶石的铬值(Cr~#60)。基于前人研究结果,提出这种现象是由于亏损方辉橄榄岩与含钛、铝、钙熔体发生交代作用,从而形成橄榄石的固溶体存在Ti~(4+)、Al~(3+)、Ca~(2+)、Fe~(3+),而Cr~(3+)进入铬尖晶石结晶。后期由于岩体在抬升过程中降温,橄榄石中出溶形成单斜辉石和磁铁矿,而铬尖晶石结晶被保留。依据铬尖晶石-橄榄石的矿物化学成分,识别出低铬纯橄岩具有最低的部分熔融程度为~8%,Cr~#最高的纯橄岩壳其部分熔融程度达到~50%,表明不同岩相间其形成背景存在明显差异。因此,笔者认为普兰蛇绿岩具有多阶段的过程。首先是在洋中脊环境下经历富Ca、Al和Na等熔体交代和部分熔融作用,后受到俯冲带玻安质熔体改造。     

内蒙古中部红格尔图地区花岗岩的成因及构造背景——LA-ICP-MS锆石U-Pb年龄、地球化学的制约

内蒙古察右后旗红格尔图花岗岩岩体位于索伦缝合带以南,主要为正长花岗岩和二长花岗岩,富硅(70.44%~78.80%),富碱(7.46%~10.74%),贫镁、铁、钛等,A/CNK值在0.95~1.41之间,碱铝指数AKI值在0.68~0.97之间,碱度率AR值在3.30~6.68之间,为弱过铝质-过铝质类碱性系列花岗岩;稀土元素总量变化范围大,轻稀土元素富集,重稀土元素亏损,Eu呈负异常(δEu=0.03~0.89);富集高场强元素Th、U、Hf、Ta、Y等,亏损大离子亲石元素Sr、Ba、Eu等;高场强元素和值((Zr+Nb+Ce+Y)350×10~(-6))明显偏低,该岩体属于高分异I型花岗岩,形成于后造山(后碰撞)伸展构造环境。LA-ICP-MS锆石同位素测年,获得锆石~(206)Pb/~(238)U年龄加权平均值分别为267.2±1.4Ma、269.2±1.6Ma和272.1±1.2Ma,表明该岩体形成于中二叠世,因此研究区内两大板块碰撞缝合的时间应该至少早于该岩体的形成时代,即应该至少早于267.2~272.1Ma。     

东喜马拉雅构造结东、西边界断裂对比及其构造演化过程

为了查明东喜马拉雅构造结东、西边界断裂的关系,及其印度与欧亚板块碰撞以来东喜马拉雅构造结的构造演化过程.在综合野外填图、构造观察、代表性岩石的锆石LA-ICP-MS U-Pb测年分析及前人研究的基础上,对东构造结两条边界断裂的几何学、运动学特征进行对比,讨论了两条断裂的多期次、多阶段的变形特征,还探讨了在东构造结地区自印度板块-欧亚板块碰撞以来的演化历史.结果显示,东构造结两条边界断裂几何学和运动学非常相似,构造变形具有明显的同时代、同期次特点,共同经历从碰撞、持续俯冲-折返、直到后期垮塌-隆升等一系列重要的地质事件.      

用E500编写简单的显示程序

用Ｅ５００编写简单的显示程序清华大学土木系测量教研室张远智，刘钊ＳＨＡＲＰＰＣ一Ｅ５００由于在系统功能、硬件配置、输入输出系统及语言环境等方面较ＰＣ一１５００有所改进，所以逐渐受到人们的关注。这其中，最引人注目的是内存容量的扩展和文件操作功能的引进，...     

St. Vincent and the Grenadines

Saint Vincent and the Grenadines conjures up images of a yachting haven and a quiet tourists' paradise. The conflicting demands of a growing population, a middle-income economy dominated by fishing, plantation agriculture and tourism, and environmental and social concerns, all contribute to marine stress on the limited, precious, but internationally important resources. While the vision exists to manage effectively coastal and offshore resources, the institutional, financial and social capital to achieve that vision is limited. Development of the fledgling partnerships between local communities, national governmental structures and the international research, government and donor organisations seems the best hope to conserve the environment and coastal livelihoods of the islands.