Microalgae, a sustainable source of multi beneficial components has been discovered and could be utilised in pharmaceutical, bioenergy and food applications. This study aims to investigate the sugaring-out effect on the recovery of protein from wet green microalga, Chlorella sorokiniana CY1 which was assisted with sonication. A comparison of monosaccharides and disaccharides as one of the phaseforming constituents shows that the monosaccharides, glucose was the most suitable sugar in forming the phases with acetonitrile to enhance the production of protein(52% of protein). The protein productivity of microalgae was found to be significantly influenced by the volume ratio of both phases, as the yield of protein increased to 77%. The interval time between the sonication as well as the sonication modes were influencing the protein productivity as well. The optimum protein productivity was obtained with 10 s of resting time in between sonication. Pulse mode of sonication was suitable to break down the cell wall of microalgae compared to continuous mode as a lower protein yield was obtained with the application of continuous mode. The optimum condition for protein extraction were found as followed: 200 g/L glucose as bottom phase with volume ratio of I:1.25, 10 s of resting time for ultrasonication, 5 s of ultrasonication in pulse mode and 0.25 g of biomass weight. The high yield of protein about 81% could be obtained from microalgae which demonstrates the potential of this source and expected to play an important role in the future. 相似文献
Water tank experiments were performed in order to investigate the behaviour of currents in pockmarks. A particle-seeded flow was visualised and quantified with the aid of the particle tracking velocimetry technique. The employed analogue pockmark is a 1:100 idealised scale model of a natural pockmark, while the highest Reynolds number in the experiments was one order of magnitude smaller than in nature. Interaction of the flow with the pockmark geometry resulted in an upwelling current downstream of the pockmark centre, along with enhanced water turbulence in the depression. Scaling-up the experimental measurements, it is found that the upwelling would be capable of preventing the settling of particles as large as very fine sand. Furthermore, the increased turbulence would support the suspended fine material, which can thus be transported away before settling. The net effect for a variable-direction near-bed current over long periods of time would be to winnow the settling sediments and reduce the sedimentation rate in pockmarks. These mechanisms may be responsible for the observed lack of sediment infill and the typical presence of relatively coarser sediments inside pockmarks compared to the surrounding bed. In contrast, sediments transported as bedload are likely to be deposited in pockmarks because of the weakening of near-bed currents as well as lateral flow convergence associated with the upwelling. Bedload, however, may not be the dominant mode of sediment transport in areas covered by cohesive sediments, where pockmarks are found. 相似文献
Suspended solids found in porewaters obtained in waterlogged soil sequences that included representative laterite–podzol transitions, associated brooks and major rivers of the Rio Negro watershed (Brazil) were studied using electron paramagnetic resonance (EPR) and Fourier-transform infrared spectroscopies. The main goal was to ascertain sources and track the evolution of suspended matter using a ubiquitous chemical species, FeIII complexed to organic matter (FeOM). Three size fractions were separated by tangential-flow (ultra)filtration: particulate (> 0.2 μm), dense (P) and light (Ps), and colloidal (5 kD < Col. < 0.2 μm) fractions. Quantitative results were acquired for Col. and Ps fractions which are predominantly organic in nature.FeOM concentration (in ‰ dry weight) was determined to be relatively low in suspended solids found in black waters from podzol porewaters and brooks whereas in the main rivers it was several times higher. FeOM concentrations were also correlated with Fe(II)/Fe(III) ratios in solution; these ratios were high in podzol porewaters and low in the rivers. Considering that organic complexation of Fe(II) is minor when compared to that of Fe(III), two interpretations were proposed to account for the above observation. First, [FeOM] was assumed to be distributed along a mixing line, with the clear waters from laterites and the black waters from podzols being its end-members. Consequently, [FeOM] can be used to trace the source of suspended material. Second, dissolved Fe(II) from podzol areas was considered to be progressively oxidized as pore waters move towards the mainstream. According to this mechanism, iron is complexed by organic matter or precipitated as oxides, thus producing an evolution of colloidal matter. As a result of these mechanisms' action, both the high production of Fe(II) and organic matter at the waterlogged podzol–laterite transition areas are major factors affecting iron export in the Rio Negro watershed. 相似文献
In some ore deposits, the grade distribution is heavy-tailed and high values make the inference of first- and second-order statistics nonrobust. In the case of gold data, high values are usually cut and the block estimation is performed using truncated grades. With this method, a part of the deposit is omitted, resulting in a potential bias on resources figures. Ad-hoc corrections can be applied on the final figures to take into account the tail of the grade distribution, but no theoretical guidelines are available. A geostatistical model has been developed to handle high values based on the assumption that for high grade zones, the only tangible information is the geometry. The grade variable z can be split into three parts: the truncated grade ($\operatorname{Min} (z, z_{\mathrm{e}})$ where ze is the top-cut grade), a weighted indicator above top-cut grade (1{z≥ze}), and a residual. Within this framework, the residual is poorly structured, and in most cases is a pure nugget-effect. Moreover, it has no spatial correlation with the truncated grade and the indicator above top-cut grade. This decomposition makes the variographic study more robust because variables (indicator and truncated grade) do not keep high grade values. The underlying hypotheses can be tested on experimental indicator variograms and the top-cut grade can be justified. Finally, the estimation is based on a truncated grade and indicator cokriging. The model is applied to blast holes from a gold deposit and on a simulated example. Both cases illustrate the benefits of keeping the high values in the estimation process via an appropriate mathematical model. 相似文献
The role of fluids in the deformation of continental serpentinites is investigated from structural, microstructural and petrographic analyses applied to a km-scale porphyroclast mantled in a viscous matrix of amphibolites. The clast is sited within a shear zone of the Palaeozoic Maures massif, France. Syntectonic fluid–rock interactions occurred from km to mm scales, at first on the clast borders (along the main rheological boundaries) then within the clast. They are accommodated macroscopically by slickenfibers faults and microscopically by shear microcracks within crack-seal veins, typifying an intermediate, brittle–ductile behaviour of serpentinites. Three main stages of deformation–serpentinisation processes occurred in relation with the left-lateral movement of the hosted shear zone. They developed under metamorphic conditions evolving from amphibolites to green-schists facies conditions ( 400 MPa/550 °C to 200 MPa/< 300 °C), as inferred from the surrounding sheared amphibolites. Deformation and serpentinisation increase through time although fluid pressure decreases. If the shape of the inclusion and its orientation relative to the shear zone mainly controlled the deformation pattern though time (P then R' shears), fluid pressure is required for starting deformation–serpentinisation processes along inherited anisotropy planes. Whatever the origin of fluids, they play a key role all along the deformation processes by influencing stress states within the shear zone at the onset of deformation and by changing at various scales and through time behaviour of the rock, depending of the intensity of serpentinisation and the rate of deformation. 相似文献
Evapotranspiration estimation is of crucial importance in arid and hyper-arid regions, which suffer from water shortage, increasing dryness and heat. A modeling study is reported here to cross-station assessment between hyper-arid and humid conditions. The derived equations estimate ET0 values based on temperature-, radiation-, and mass transfer-based configurations. Using data from two meteorological stations in a hyper-arid region of Iran and two meteorological stations in a humid region of Spain, different local and cross-station approaches are applied for developing and validating the derived equations. The comparison of the gene expression programming (GEP)-based-derived equations with corresponding empirical-semi empirical ET0 estimation equations reveals the superiority of new formulas in comparison with the corresponding empirical equations. Therefore, the derived models can be successfully applied in these hyper-arid and humid regions as well as similar climatic contexts especially in data-lack situations. The results also show that when relying on proper input configurations, cross-station might be a promising alternative for locally trained models for the stations with data scarcity.
Petrographic and geochemical studies of peridotites and melagabbros from the Maures massif (SE France) provide new constraints
on the Early Palaeozoic evolution of the continental lithosphere in Western Europe. Peridotites occur as lenses along a unit
rooted in the main Variscan suture zone. They are dominantly spinel peridotites and minor garnet–spinel peridotites. Spinel
peridotites represent both residual mantle and ultramafic cumulates. Mantle-related dunites and harzburgites display high
temperature textures, with olivine (Mg#0.90), orthopyroxene (Mg#0.90) and spinel (TiO2 < 0.2%; Cr#0.64–0.83) compositions typical of fore-arc upper mantle. Ultramafic cumulates are dunite adcumulates, harzburgite heteradcumulates
and mesocumulates, melagabbro heteradcumulates and amphibole peridotites, with olivine (Mg#0.85–0.89), orthopyroxene (Mg#0.86–0.89) and Cr-spinel (TiO2 = 0.5–3.3%; Cr#0.7–0.98) compositions typical of ultramafic cumulates. Cr-spinel compositions of both spinel peridotite types suggest their genesis
in a supra-subduction zone lithosphere. Core to rim zoning in spinel is related to the incomplete influence of regional metamorphism
and serpentinisation. The covariation of major and minor elements with Al2O3 for cumulates is consistent with igneous processes involving crystal accumulation. Both mantle and cumulate dunites and harzburgites
have U-shaped REE patterns and extremely low trace element contents, similar to peridotites from modern fore-arc peridotites
(South Atlantic) and from ophiolites related to supra-subduction zones (Semail, Cyclops, Pindos, Troodos). Melagabbros also
have U-shaped REE patterns similar to xenoliths from the Philippine island arc, but also similar to intrusive ultramafic cumulates
from the Semail nappe of Oman related to a proto-subduction setting. A wehrlite has a REE pattern similar to that of amphibole
peridotites reflecting metasomatism of clinopyroxene-bearing peridotites due to subduction-related fluids. The Maures spinel
peridotites and melagabbros are therefore interpreted as the lowermost parts of a crustal sequence and minor residual mantle
of lithosphere generated in a supra-subduction zone during Early Palaeozoic time. Garnet–spinel peridotites are chemically
close to melagabbros, but have recorded high pressure metamorphism before their retrogression similar to spinel peridotites
into amphibolites to greenschists facies metamorphism. They indicate burial to mantle depths of the margin of the supra-subduction
lithosphere during the Early Palaeozoic continental subduction. Both peridotite types were exhumed during the Upper Palaeozoic
continental collision. Comparable observations from other Variscan-related peridotites, in particular of the Speik complex
of the Autroalpine basement, and a common age for the subduction stage allow extension of these regional conclusions to a
broad area sharing the Cambrian suture zone, extending from the Ossa-Morena to the Bohemian massif. 相似文献
Monitoring of the near-nucleus activity of C/1995 O1 (Hale-Bopp) began in Teide Observatory in August 1995. During 1996 the
comet was observed on 72 nights between March 26 and November 13. A permanent fan structure was observed towards the north
during the whole period of observation. The position angle of the axis of this fan was measured and its variations with time
were used to determine the position of the North Pole of the cometary nucleus.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
The Imaging Magnetograph eXperiment (IMaX) is a spectropolarimeter built by four institutions in Spain that flew on board the Sunrise balloon-borne solar observatory in June 2009 for almost six days over the Arctic Circle. As a polarimeter, IMaX uses fast polarization modulation (based on the use of two liquid crystal retarders), real-time image accumulation, and dual-beam polarimetry to reach polarization sensitivities of 0.1%. As a spectrograph, the instrument uses a LiNbO3 etalon in double pass and a narrow band pre-filter to achieve a spectral resolution of 85 mÅ. IMaX uses the high-Zeeman-sensitive line of Fe i at 5250.2 Å and observes all four Stokes parameters at various points inside the spectral line. This allows vector magnetograms, Dopplergrams, and intensity frames to be produced that, after reconstruction, reach spatial resolutions in the 0.15??C?0.18 arcsec range over a 50×50 arcsec field of view. Time cadences vary between 10 and 33 s, although the shortest one only includes longitudinal polarimetry. The spectral line is sampled in various ways depending on the applied observing mode, from just two points inside the line to 11 of them. All observing modes include one extra wavelength point in the nearby continuum. Gauss equivalent sensitivities are 4 G for longitudinal fields and 80 G for transverse fields per wavelength sample. The line-of-sight velocities are estimated with statistical errors of the order of 5??C?40 m?s?1. The design, calibration, and integration phases of the instrument, together with the implemented data reduction scheme, are described in some detail. 相似文献