Application of Atomic Force Microscopy to Observation of Marine Bacteria

The atomic force microscopy (AFM) is a recently developed, bench-top instrument that can image the surface structures of biological specimens at high resolution with simultaneous measurement of their size. This paper describes the application of AFM to marine bacteria. Both natural and cultured bacteria were retained on a filter or placed on glass, washed, air-dried and observed by AFM. The instrumental condition, the choice of suitable filter, effect of fixation and filtration, comparison with epifluorescent microscopic (EFM) count, and the size and shape of bacterial cells were investigated. An Isopore filter was best for concentration and subsequent observation because of its surface flatness. Cross section images showed that both rod and coccoid cells were flattened, the former usually having a two-humped shape. Bacterial cells were differentiated from non-living particles based on their cross section shape and size. Bacterial counts by AFM and EFM showed good agreement. Although size measurement is easily done by the instrument, AFM tends to overestimate the size of microspheres. More work is thus needed on the size measurement of living organisms. Because AFM easily provides images of natural bacterial cells at high magnification, it can be used as a new tool to study the fine structures of marine bacteria. This revised version was published online in July 2006 with corrections to the Cover Date.     

The squeezing potential of rock around tunnels: Theory and prediction with examples taken from Japan

Summary Large deformations of surrounding media around tunnels are often encountered during excavations in rocks with squeezing characteristics. These deformations may sometimes continue for a long period of time. Predictions of deformations of tunnels in such grounds are urgently needed, not because of stability concerns, but also of their sevicability. In the present study, the squeezing phenomenon of rock around tunnels and its mechanism and associated factors are first clarified by carefully studying failures of tunnels, and a survey of tunnels in squeezing rocks in Japan is presented and its results are summarised. Then, a practical method is proposed to predic the squeezing potential and deformation of tunnels in squeezing rock and this method has beeen applied to actual tunnelling projects, where squeezing problems were encountered, to check its applicability and validity. Finally, an extension of this method to the time-dependent behaviour of squeezing rocks is given and an application of this method to an actual tunnel is presented.     

Effects of grain morphology on critical state: a computational analysis

We introduce a new DEM scheme (LS-DEM) that takes advantage of level sets to enable the inclusion of real grain shapes into a classical discrete element method. Then, LS-DEM is validated and calibrated with respect to real experimental results. Finally, we exploit part of LS-DEM potentiality by using it to study the dependency of critical state (CS) parameters such as critical state line (CSL) slope \(\lambda \), CSL intercept \(\varGamma \), and CS friction angle \(\varPhi _{\mathrm{CS}}\) on the grain’s morphology, i.e., sphericity, roundness, and regularity. This study is carried out in three steps. First, LS-DEM is used to capture and simulate the shape of five different two-dimensional cross sections of real grains, which have been previously classified according to the aforementioned morphological features. Second, the same LS-DEM simulations are carried out for idealized/simplified grains, which are morphologically equivalent to their real counterparts. Third, the results of real and idealized grains are compared, so the effect of “imperfections” on real particles is isolated. Finally, trends for the CS parameters (CSP) dependency on sphericity, roundness, and regularity are obtained as well as analyzed. The main observations and remarks connecting particle’s morphology, particle’s idealization, and CSP are summarized in a table that is attempted to help in keeping a general picture of the analysis, results, and corresponding implications.     

The squeezing potential of rocks around tunnels; Theory and prediction

Summary The deformational behaviour of tunnels, which underwent large deformations, socalled squeezing, have been recently receiving great attention in the field of rock mechanics and tunnelling. Contrary to rockbursting phenomenon in which the deformation of the medium takes place instantaneously, the deformation of the surrounding rock in squeezing phenomenon takes place slowly and gradually when the resulting stress state following the excavation exceeds the strength of the surrounding medium. Although there are some proposals for the definition of squeezing rocks and prediction of their squeezing potential and deformations of tunnels in literature, it is difficult to say that they are concise and appropriate.In the first half of this paper, the squeezing phenomenon of rock about tunnels and its mechanism and associated factors are clarified by studying carefully observed failures in-situ and laboratory model tests. Then, an extensive survey of tunnels in squeezing rocks in Japan is presented and the results of this survey are summarised. In the second half of the paper, a new method is proposed to predict the squeezing potential and deformations of tunnels in squeezing rock. Then, the method is applied to actual tunnelling projects, where squeezing problems have been encountered, to check its validity and applicability. As a concrete example, an application of the method to predict the squeezing potential and deformations of the rock along a 300 m long section of an actual tunnel was made.     

Fate and transport of pollutants through a municipal solid waste landfill leachate in Sri Lanka

This study focuses on the characterization of leachate generated from Gohagoda dumpsite in Kandy, Sri Lanka, assessment of its spatial and temporal variations, and identification of subsurface canals and perched water bodies in the wetland system affected by the leachate flow. Leachate samples were collected monthly throughout dry and rainy seasons from different points of the leachate drainage channel over a period of 1 year and they were tested for quality parameters: pH, temperature, electrical conductivity, total dissolved soils, alkalinity, hardness, total solids, volatile solids, total suspended solids, volatile suspended solids, biochemical oxygen demand (BOD₅), chemical oxygen demand, nitrate-nitrogen, nitrite-nitrogen, phosphates, ammonium-nitrogen, chloride, dissolved organic carbon, total organic carbon and heavy metals. Sequential soil extraction procedures were performed for the characterization of leachate-affected local soil. A geophysical survey using direct current resistivity technique was conducted at locations downstream of the dumpsite. Leachate characteristics indicated that the leachate is in the methanogenic phase and the results strongly suggest that the leachate may be polluting the river where the leachate is discharged directly. Leachate exceeds the allowable limits of Sri Lankan wastewater discharge standards for many of the parameters. Significant difference (P < 0.05) was observed for most of organic and inorganic parameters among all sampling locations. Many parameters showed a negative correlation with pH. The affected soils showed high heavy metal concentrations. Resistivity study confirmed a confined leachate flow at the near surface with few subsurface canals. However, no separate subsurface plume movement was observed. The results of this research can effectively be used for the establishment of an efficient and effective treatment method for the Gohagoda landfill leachate.     

Melt inclusion analysis of the Unzen 1991–1995 dacite: implications for crystallization processes of dacite magma

Dacitic magma, a mixture of high-temperature (T) aphyric magma and low-T crystal-rich magma, was erupted during the 1991–1995 Mount Unzen eruptive cycle. Here, the crystallization processes of the low-T magma were examined on the basis of melt inclusion analysis and phase relationships. Variation in water content of the melt inclusions (5.1–7.2 wt% H₂O) reflected the degassing history of the low-T magma ascending from deeper levels (250 MPa) to a shallow magma chamber (140 MPa). The ascent rate of the low-T magma decreased markedly towards the emplacement level as crystal content increased. Cooling of magma as well as degassing-induced undercooling drove crystallization. With the decreasing ascent rate, degassing-induced undercooling decreased in importance, and cooling became more instrumental in crystallization, causing local and rapid crystallization along the margin of the magma body. Some crystals contain scores of melt inclusions, whereas there are some crystals without any inclusions. This heterogeneous distribution suggests the variation in the crystallization rate within the magma body; it also suggests that cooling was dominant cause for melt entrapment. Numerical calculations of the cooling magma body suggest that cooling caused rapid crystal growth and enhanced melt entrapment once the magma became a crystal-rich mush with evolved interstitial melt. The rhyolitic composition of melt inclusions is consistent with this model.Editorial responsibility: H Shinohara     

New technologies and systems for high quality citrus fruit production,labor-saving and orchard construction in mountain areas of Japan

Novel technologies have been necessary for improving fruit quality and productivity of citrus,labor-saving and orchard conservation on steep slope lands since aging of growers and decrease in the number of successors is remarkable in mountain areas of southwestern Japan. The purpose of this paper is to introduce new technologies for improving citrus production that have been developed in recent years. A new fruit quality control system using drip irrigation and liquid fertilization technique combined with year-round plastic mulching was developed, and it enables high quality and stable citrus fruit production. Water and/or nutrient solution is automatically supplied through drip tubes that are laid under the mulching sheets to give adequate water stress, so as to improve sugar and acid content of fruit. A new transportation system for steep sloping citrus orchards, which is a combination of the monorail system and contour narrow paths, was suggested. A small walking cultivator was developed to explain the procedure of narrow path excavation. After introducing the narrow path, working hours for fertilizer and chemicalherbicide application were reduced. Disasterpr evention mapping of citrus orchards on slope landswas developed based on computer-aided seepage estimation and topographic data. The mapping can show zones of both ascending flow and descendingflow of underground water during heavy rains incitrus orchards. The mapping is considered to be effective for the management of orchards andprevention of erosion on slope lands.     

Multi-scale limit load analysis for discontinuous rock mass based on the homogenization method

A new method to evaluate the strength of rock mass structures is proposed and examined. The method is based on the collapse load analysis of elasto-perfectly plastic material along with the homogenization method, which enables the multi-scale analyses for heterogeneous media. The homogenization process replaces a rock mass with cracks by an equivalent continuum medium with macroscopic stiffness while the failure criterion for the rock mass is estimated in the localization process. It is shown that both the averaged stiffness and the macroscopic failure criterion of the discontinuous rock mass are numerically obtained via the finite element analyses. Thus, the failure strength of a rock mass structure is evaluated by the collapse load analysis in the form of Linear Programming with the macroscopic failure criterion. This is the first attempt to apply the homogenization method to the strength analysis of rock mass. Copyright © 1999 John Wiley & Sons, Ltd.