地温场数值模拟软件包

为了实现多年来我国地热数据的挽救和共享，我们开发了“大地热流数据库”，该库的开发使用了Microsoft Visual C++6.0 和Visual Basic 6.0作为编成语言.该库是“中国岩石圈三维结构数据库”的子库之一.该库具备对原始数据的查询、增加、删除和编辑和地温场的数值模拟，针对岩石圈范围内的地质体，比如对地下一维、二维和三维温度场的有限元数值模拟，以方便地热工作者解决一些常见的地热问题.     

基于GIS 平台的中国大地热流数据库

中国大地热流数据库是中国岩石圈三维结构数据库的子库之一。该库底层数据库为Access 97,基于地理信息系统GIS平台,应用MO、 DAO及API技术,使用VB、 VC语言实现了数据库的各项功能。该库具有对原始数据进行添加、删除、编辑、备份、浏览、查询、显示和维护等功能,并且开发了常规的数据解释软件包,还可对原始数据进行一些常规处理,同时任意输出各种数据报表和图件等。另外,还开发有中英文双语言界面。     

Molecular cloning and mRNA expression analysis of myosin heavy chain (MyHC) from fast skeletal muscle of grass carp, Ctenopharyngodon idella

The myosin heavy chain (MyHC) is one of the major structural and contracting proteins of muscle. We have isolated the cDNA clone encoding MyHC of the grass carp, Ctenopharyngodon idella. The sequence comprises 5 934 bp, including a 5 814 bp open reading frame encoding an amino acid sequence of 1 937 residues. The deduced amino acid sequence showed 69% homology to rabbit fast skeletal MyHC and 73%–76% homology to the MyHCs from the mandarin fish, walleye pollack, white croaker, chum salmon, and carp. The putative sequences of subfragment-1 and the light meromyosin region showed 61.4%–80% homology to the corresponding regions of other fish MyHCs. The tissue-specific and developmental stage-specific expressions of the MyHC gene were analyzed by quantitative real-time PCR. The MyHC gene showed the highest expression in the muscles compared with the kidney, spleen and intestine. Developmentally, there was a gradual increase in MyHC mRNA expression from the neural formation stage to the tail bud stage. The highest expression was detected in hatching larva. Our work on the MyHC gene from the grass carp has provided useful information for fish molecular biology and fish genomics.     

Molecular cloning and mRNA expression analysis of myosin heavy chain (MyHC) from fast skeletal muscle of grass carp, Ctenopharyngodon idella

The myosin heavy chain(MyHC)is one of the major structural and contracting proteins of muscle.We have isolated the cDNA clone encoding MyHC of the grass carp,Ctenopharyngodon idella. The sequence comprises 5 934 bp,including a 5 814 bp open reading frame encoding an amino acid sequence of 1 937 residues.The deduced amino acid sequence showed 69%homology to rabbit fast skeletal MyHC and 73%–76%homology to the MyHCs from the mandarin fish,walleye pollack,white croaker,chum salmon,and carp.The putative sequences of subfragment-1 and the light meromyosin region showed 61.4%–80%homology to the corresponding regions of other fish MyHCs.The tissue-specific and developmental stage-specific expressions of the MyHC gene were analyzed by quantitative real-time PCR.The MyHC gene showed the highest expression in the muscles compared with the kidney,spleen and intestine.Developmentally,there was a gradual increase in MyHC mRNA expression from the neural formation stage to the tail bud stage.The highest expression was detected in hatching larva.Our work on the MyHC gene from the grass carp has provided useful information for fish molecular biology and fish genomics.