The effects of electrochemical oxidation on in-vivo fluorescence and toxin content in Microcystis aeruginosa culture

The increasing occurrence of cyanobacterial blooms in water bodies is a serious threat to the environment. Efficient in-lake treatment methods for the control of cyanobacteria proliferation are needed, their in-vivo detection to obtain a real-time response to their presence, as well as the information about their physiological state after the applied treatment. In-vivo fluorescence measurements of photosynthetic pigments have proved to be effective for quantitative and qualitative detection of phytoplankton in a water environment. In the experiment, chlorophyll and phycocyanin fluorescence sensors were used concurrently to detect stress caused by electrochemical oxidation applying an electrolytic cell equipped with borondoped diamond electrodes on a laboratory culture of cyanobacteria Microcystis aeruginosa PCC 7806. The inflicted injuries were reflected in a clear transient increase in the phycocyanin fluorescence signal(for 104 %? 43%) 24 h after the treatment, which was not the case for the chlorophyll fluorescence signal. In the next 72 h of observation, the fluorescence signals decreased(on 40% of the starting signal) indicating a reduction of cell number, which was confirmed by cell count(24% reduction of the starting concentration) and analysis of extracted chlorophyll and phycocyanin pigment. These results demonstrate the viability of the combined application of two sensors as a useful tool for in-vivo detection of induced stress, providing real-time information needed for the evaluation of the efficiency of the in-lake treatment and decision upon the necessity of its repetition. The electrochemical treatment also resulted in a lower free microcystins concentration compared to control.     

Design of Buoys for Mounting Wind Turbines at Exposed Sites

In this study, two designs for a buoy capable of supporting a 10 kW wind turbine and its tower were developed to operate at the University of New Hampshire’s Center of Ocean Renewable Energy testing site located off the Isles of Shoals, New Hampshire. The buoys are to be moored by a catenary chain system. To evaluate wave response, two Froude-scaled models were constructed, tested, and compared at the Ocean Engineering wave tank at the University of New Hampshire. These buoys have been implemented and compared with wave tank measurements of the spar displacement at a reference elevation 2.44 m above the mean water level.     

不饱和古罗糖醛酸三糖和甘露糖醛酸三糖的结构表征

以双功能褐藻胶裂解酶 (Pseudoalteromonas sp.strain No. 2 72 )分别降解均聚古罗糖醛酸(PG)和均聚甘露糖醛酸 (PM) ,经 Bio- Gel- P6和 POROS- HQ2 0分离得到 2种三糖纯品 ,经 ESI- MS,1 H- NMR,1 3 C- NMR,1 H- 1 H COSY,1 H- 1 3 C HMQC确定其结构为 Δ4,5-古罗糖醛酸 - α(1→ 4 ) - L-古罗糖醛酸 -α(1→ 4 ) - L -古罗糖醛酸 (简写为Δ GG)和Δ4,5-甘露糖醛酸 -β (1→ 4 ) - D-甘露糖醛酸 -β (1→ 4 ) - D-甘露糖醛酸 (ΔMM) ,为进一步从事构效关系研究提供结构信息。     

珠江口磨刀门整治前后水动力数值模拟

磨刀门是珠江的主要泄洪通道之一,径流分配居珠江三角洲八大口门之首。利用磨刀门1977年地形(大规模整治前)和2003年的地形,通过ECOMSED模型模拟了磨刀门海域洪枯季节水动力场,对潮流、余流、潮能通量特性进行了对比,发现整治后磨刀门水动力强度加大,而且流速滩槽分异明显,余流场与落潮流方向一致。20世纪70年代,余流自口门出来后在内海区右偏,现在磨刀门水道余流偏向西部浅滩;潮能通量密度加大,滩槽分布差异明显。     

目标采集技术在西部新区地震勘探中的应用

目标采集技术是根据复杂的地下地质构造提出来的,它是针对诸如小断层、小断块、复杂断裂带、特殊岩性、泥岩裂缝等主要地质构造目标而进行的有针对性的目标观测系统设计及目标采集施工方法设计的技术。针对目标采集的特点,在观测系统设计中提出了以地质模型正演模拟分析为核心的观测系统设计技术;在老资料分析和采集参数论证中提出了细化、量化分析技术。通过目标采集技术在西部探区的应用,有效的解决了西部新区勘探开发中广泛存在的复杂隐蔽油气藏勘探难题。     

Taxonomical and Biogeochemical Investigation of Elements in Holocene Mollusk Shells in the South and Southwestern Marmara Basin,Turkey

Concentrations of some heavy metals and trace elements such as Cr, Ga, Ni, Zn, Mo, Cu, Pb, Yb, Y, Nb, Ti, Sr, Ba, Mn, Sc, Co, V, Zr, Fe, Al, W, Se, Bi, Sb, As, Cd in recent mollusk shells and factors affecting their distribution and deposits collected from various depths in the southern and southwestern parts of the Marmara Sea are investigated. The distribution of the elements in the shells is categorized into four groups. Of these, concentrations of 12 elements （As, Bi, Cd, Co, Ga, Mo, Nb, Sb, Se, Sc, W and Yb） are below zero [（0.053-0.79）×10^-6]; concentrations of seven elements （Cr, Ni, Pb, V, Y, Zr and Cu） are （1.0-6.0）×10^-6; concentrations of four elements （Ti, Mn, Ba and Zn） are 10- 20×10^-6; and concentrations of five elements （Si, Al, Fe, Mg and Sr） are （47.44-268.11）×10^-6. The taxonomic characteristics of the 29 elements were studied separately in mollusk shells such as Chamalea gallina （Linn6）, Pitar rudis （Poli）, Nassarius reticulatus （Linn6）, Venerupis senescens （Coocconi）, Mytilus galloprovincialis （Lamarck）, Mytilaster lineatus （Gemelin in Linne） and Chlamys glabra. It was found that, in mollusk taxonomy, the elements have unique values. In other words, element concentrations in various mollusk shells depend mainly on the taxonomic characteristics of mollusks. In various bionomic environments different element distributions of the same species are attributed to the different geochemical characters of the each environment. Data obtained in this study indicate that the organisms are the most active and deterministic factors of the environment.     

三种类型烃-烃不混溶包裹体组合的特征和热力学条件的计算

在自然界广泛分布着烃-烃不混溶体系中捕获的流体包裹体,由于这些包裹体具有复杂的组成和相态,因此不混溶包裹体组合的判别和热力学参数的计算常常难以进行。根据烃-烃不混溶体系中两个端员组分流体包裹体室温下的相态特征和在温度-压力平面图上等容线交点显示的位置,划分成三种类型流体包裹体组合,本介绍了三种类型流体包裹体组合特征,叙述了不混溶烃-烃包裹体组合的测定和判别方法,并且阐述均一化包裹体相态方程和气-液平衡常数原理和方法与此同时列举了自然界简单的三种类型不混溶烃-烃包裹体组合的测定、判别和计算的几个实例,利用相态方程和气-液平衡常数,不但精确地计算出包裹体均一压力,并且精确地计算出流体密度和体积等热力学参数。最后,利用均一成气相和液相的两种包裹体在 p-T 平面图上等容线交点同样计算出流体包裹体组合的捕获温度和压力。     

饱和岩石的各向异性及非线性黏弹性响应

在MTS伺服压机上对不同饱和状态的砂岩、大理岩标本进行了垂直层理和平行层理两个方向的正弦波加载试验,研究了饱和岩石的各向异性及非线性黏弹性行为. 在最大载荷低于岩石的屈服点时,获得的衰减、杨氏模量、泊松比、波速都表现出显著的各向异性,同时它们还具有较明显的应变振幅效应和频率效应. 随着应变振幅的增大,衰减近似呈线性增长,杨氏模量和泊松比近似呈线性下降. 在0.005-4Hz频段内除衰减不明显依赖频率以外,杨氏模量、泊松比、波速对频率的依赖性都较强; 当频段扩大到15Hz时,这4个参数都表现出较强的频率效应. 饱和岩石的衰减、杨氏模量、泊松比、波速均随饱和液体的黏滞系数增大而增大.     

湖南省1：1万土地利用现状数据库系统应用研究

本文介绍了湖南省1:1万土地利用现状数据库建设工程自2000年启动以来, 在土地变更调查及数据统计汇总、土地资源调查评价、农村集体土地所有权登记发证、制定土地开发整理规划、建设用地审批和土地监察等方面的应用,分析了土地利用现状数据库应用中存在的问题,并提出了一些解决办法。     

工程岩体三维构模中钻孔数据处理方法

原始数据处理是工程岩体三维构模中存在的主要困难之一。从三维构模的角度,将工程岩体按几何特征抽象为4种结构单元类型。钻孔数据是描述工程岩体的重要原始数据,具有多尺度性。针对这个特点,提出钻孔数据纵向概化准则,解决工程岩体多尺度显示问题;根据地质学的地质数据解译方法,结合岩层层状分布的特点,提出层处理方法对钻孔数据进行概化处理,确定工程岩体所对应的结构单元类型,创建工程岩体结构单元三维构模数据,建立由钻孔数据所得到的工程岩体三维模型。