四川省地县分组交换网系统的设置和安装

介绍了四川我省地(市)县气象局通过X.25网络连接交换数据其服务器端和客户端x.25卡设置和操作系统有关参数的调整.     

一种水产动物病毒现场检测免疫芯片的制备与应用

采用原子力显微镜对氨基化玻片及6种不同方法修饰玻片进行表面特征分析,并比较了硝酸纤维素(NC)膜、PVDF膜以及以上不同修饰玻片对抗体的固定效率、固定效果,最终选择琼脂糖修饰玻片作为芯片载体。将纯化后的兔抗血清(捕获抗体)点样至琼脂糖修饰玻片上,制备免疫芯片,与待检样品(病毒感染的靶器官组织)匀浆液孵育形成复合物,该复合物被辣根过氧化物酶(HRP)标记的特异性单克隆抗体(单抗)识别,经底物显色,得到肉眼可见的检测结果。通过改变芯片制备及应用过程中的具体条件参数,对各条件进行了优化,并采用生物素-链亲和素(BAS)标记特异性单抗作为检测抗体以提高检测灵敏度。基于此夹心免疫分析原理制备的WSSV、LCDV现场检测免疫芯片,病毒的最低检出量分别为82.50ng/ml、0.88μg/ml,在一定的抗原浓度范围内,病毒浓度的对数值与信号强度呈线性关系;采用BAS放大检测信号后,病毒的最低检出量为12.38ng/ml、0.22μg/ml。该免疫芯片与酶联免疫吸附法(ELISA)及免疫荧光法(IFAT)对同种样品的检测结果高度一致。     

综合信息系统对地级用户资料处理的研究

综合信息系统对地级用户资料处理的研究蒋丽娟，缪贵平，向继涛（四川省气象局通信台邮码６１００７２）目前，随着计算机网络通信技术的飞速发展和广泛应用，特别是去年底成都区域气象中心的成立，整个西南区域气象业务体制已逐渐由通信方式向网络方式过渡，用户由原来的...     

利用Perl开发数据库和网页

本文阐述了一个实际的开发实例，通过该文章向大家提供一种开发网页和数据库的简便快捷方法，为今后气象领域在互联网上的应用提供参考。     

气象资料的Web方式调用

本文介绍了为遂宁民航开发的一个Web产品,该产品用Windows2000作为平台,SQ SERVER 7-0为数据库,用dveam veaver 4和Front pape开发的网页,通过它可以更方便地在intevnet网上调用和显示数据库资料.     

四川省地县分组交换网系统的设置和安装

介绍了四川我省地（市）县气象局通过x.25网络连接交换数据其服务器端和客户端x.25卡设置和操作系统有关参数的调整。     

气象资料的Web方式调用

本文介绍了为遂宁民航开发的一个Web产品，该产品用Windows2000作为平台，SQ SERVER 7．0为数据库，用dveam veaver 4和Front pape开发的网页，通过它可以更方便地在internet网上调用和显示数据库资料。     

Development and application of antibody microarray for white spot syndrome virus detection in shrimp

Detecting white spot syndrome virus (WSSV) in shrimp in high efficiency and veracity is important for disease prevention in aquaculture. Antibody-based microarray is a novel proteomic technology that can meet the requirements. In this study, we developed an antibody microarray for WSSV-detection in a specific and parallel way at multiple samples. First, seven slides each with different modifications were characterized by atomic force microscope, and were compared in the efficiency of immobilizing proteins. Of the seven, 3-dimensional structured agarose gel-modified slides were chosen appropriate for the microarray for having higher signal value and superior spot size. A purified rabbit anti-WSSV antibody was arrayed as the capture antibody of the microarray on the agarose gel-modified slides, and then the microarray slides were incubated in the tissue homogenate of sampled shrimp and the antibody-antigen complex was detected by Cy3-conjugated anti-WSSV monoclonal antibody. The results were measured by a laser chipscanner and analyzed with software. To obtain satisfied fluorescence signal intensity, optimal conditions were searched. The detection limit of the antibody microarray for WSSV is 0.62 μg/mL, with a proven long shelf life for 6 months at 4°C or 8 months at -20°C. Furthermore, concordance between antibody microarray and traditional indirect ELISA reached 100% for WSSV detection. These results suggest that the antibody microarray could be served as an effective tool for diagnostic and epidemiological studies of WSSV.     

利用Perl开发数据库和网页

本文阐述了一个实际的开发实例,通过该文章向大家提供一种开发网页和数据库的简便快捷方法,为今后气象领域在互联网上的应用提供参考.     

新一代天气预报电视会商系统概述

本文就四川省气象局建立的"全国天气预报电视会商系统"作一个简单的概述,让大家对气象部门的这套系统有一个了解和认识.