Induction of vitellogenin in vivo and in vitro in the model teleost medaka (Oryzias latipes): comparison of gene expression and protein levels

Quantification of the egg yolk precursor vitellogenin (VTG) in fish has become a standard technique to detect estrogenic effects of known chemicals and environmental samples. In the present study, we have analysed VTG induction by estradiol, ethynylestradiol and genistein exposure in the model teleost medaka (Oryzias latipes) and demonstrate that the medaka is a suitable model system to analyse estrogenic effects. By comparing VTG gene expression and protein levels we show that in principal both techniques can be used to study VTG induction in vivo (juvenile and adult males) and in vitro (primary cultures of male liver cells). If a short term in vivo or in vitro exposure is performed, detection of mRNA might be sufficient. For long term studies with the need to detect weak estrogenic chemicals and a precise quantification, immuno-chemical detection may be favoured.     

Temperature and its control of isotope fractionation by a sulfate-reducing bacterium

A synthesis of previous results, which we dub the “standard model,” provides a prediction as to how isotope fractionation during sulfate reduction should respond to physiological variables such as specific rate of sulfate reduction and environmental variables such as substrate availability and temperature. The standard model suggests that isotope fractionation should decrease with increasing specific rates of sulfate reduction (rate per cell). Furthermore, the standard model predicts that low fractionations should be found at both high and low temperatures whereas the highest fractionations should be found in the intermediate temperature range. These fractionation trends are controlled, as a function of temperature, by the balance between the transfer rates of sulfate into and out of the cell and the exchange between the sulfur pools internal to the organism. We test this standard model by conducting experiments on the growth physiology and isotope fractionation, as a function of temperature, by the sulfate-reducing bacterium Desulfovibrio desulfuricans (DSMZ 642). Our results contrast with the “standard model” by showing a positive correlation between specific rates of sulfate reduction and fractionation. Also by contrast with the standard model, we found the highest fractionations at low and high temperatures and the lowest fractionations in the intermediate temperature range. We develop a fractionation model which can be used to explain both our results as well as the results of the “standard model.” Differences in fractionation with temperature relate to differences in the specific temperature response of internal enzyme kinetics as well as the exchange rates of sulfate in and out of the cell. It is expected that the kinetics of these processes will show strain-specific differences.     

Luxury Tourism and Regional Economic Development in Mexico

This article argues that little attention has been paid to the territorial and socioeconomic impact of consumer–service globalization on Third World countries. It specifically examines the economic role of tourism in Mexico and its limited effect on the country's regional development. Despite governmental support in order to enhance economic growth, currency receipts, and employment, tourism contributes less than 5 percent to the gross domestic product, and the majority of tourism–related jobs are precarious and low–skilled. The promotion of luxury resorts in coastal areas has led to highly concentrated and fast–growing enclaves of mainly foreign investment. However, this strategy has failed to stimulate productive links between tourist centers and their hinterlands and has led to large–scale urbanization characterized by a considerable lack of basic services for the resident population.     

Seeing Conditions at Sutherland. Results of the 1992/93 seeing campaign

In order to decide whether the seeing conditions at SAAO/Sutherland justify the erection of a 3.5 m telescope and also to compare Sutherland with the Gamsberg/Namibia site, a seeing campaign covering 15 months has been carried out. For direct comparison with the results of the seeing campaign at Gamsberg twenty years before the same QUESTAR telescope was employed. The seeing is determined by the scattering of the star-trail exposed on a film in the focal plane of the telescope. The campaign commenced in February 1992. Up to May 1993, data for 204 nights, that is 47.3% of the total number of nights, were collected. Due to wind speeds above 30 km h^-1, 25 out of the 204 nights were not considered in the final reduction. The useful 179 nights are evenly distributed over the campaign period. The median seeing value for the whole period is = 0.52. There are differences during the year: the best season gives = 0.42, the worst = 0.67. Each night was divided into three intervals, although data for each of the three intervals were not always available. Generally, there is an improvement in the seeing during the course of a night. The results are compared to the seeing values of Gamsberg/Namibia and ESO/La Silla.     

The motion of a lunar satellite

Presented in this theory is a semianalytical solution for the problem of the motion of a satellite in orbit around the moon. The principal perturbations on such a body are due to the nonspherical gravity field of the moon, the attraction of the earth, and, to a lesser degree, the attraction of the sun. The major part of the problem is solved by means of the celebrated von Zeipel Method, first successfully applied to the motion of an artificial earth satellite by Brouwer in 1959. After eliminating from the Hamiltonian all terms with the period of the satellite and those with the period of the moon, it is suggested to solve the remaining problem with the aid of numerical integration of the modified equations of motion.This theory was written in 1964 and presented as a dissertation to Yale University in 1965. Since then a great deal has been learned about the gravity field of the moon. It seems that quite a number of recently determined gravity coefficients would qualify as small quantities of order two. Hence, according to the truncation criteria employed, they should be considered in the present theory. However, the author has not endeavored to update the work accordingly. The final results, therefore, are incomplete in the lunar gravitational perturbations. Nevertheless, the theory does give the largest such variations and it does present the methods by which perturbations may be derived for any gravity terms not actually developed.     

Planetary elements for 10 000 000 years

In 1950 Brouwer and van Woerkom published a secular theory of the variations of the planetary elements in analytical form. In the present paper we provide a graphical representation of this theory in the form of element plots for a time span of ten million years.     

Special Contributions: Pictorial Series of the Manifestations of the Dynamics of the Earth: 5. Kamchatka,Its Early Cartography and Recent Climate Changes

Studia Geophysica et Geodaetica -     

Influence of vegetation change on watershed hydrology: implications for paleoclimatic interpretation of lacustrine δ18O records

Stratigraphic shifts in the oxygen isotopic (¹⁸O) and trace element (Mg and Sr) composition of biogenic carbonate from tropical lake sediment cores are often interpreted as a proxy record of the changing relation between evaporation and precipitation (E/P). Holocene ¹⁸O and Mg and Sr records from Lakes Salpetén and Petén Itzá, Guatemala were apparently affected by drainage basin vegetation changes that influenced watershed hydrology, thereby confounding paleoclimatic interpretations. Oxygen isotope values and trace element concentrations in the two lowland lakes were greatest between ~ 9000 and 6800 ¹⁴C-yr BP, suggesting relatively high E/P, but pollen data indicate moist conditions and extensive forest cover in the early Holocene. The discrepancy between pollen- and geochemically-inferred climate conditions may be reconciled if the high early Holocene ¹⁸O and trace element values were controlled principally by low surface runoff and groundwater flow to the lake, rather than high E/P. Dense forest cover in the early Holocene would have increased evapotranspiration and soil moisture storage, thereby reducing delivery of meteoric water to the lakes. Carbonate ¹⁸O and Mg and Sr decreased between 7200 and 3500 ¹⁴C-yr BP in Lake Salpetén and between 6800 and 5000 ¹⁴C-yr BP in Lake Petén Itzá. This decline coincided with palynologically documented forest loss that may have led to increased surface and groundwater flow to the lakes. In Lake Salpetén, minimum ¹⁸O values (i.e., high lake levels) occurred between 3500 and 1800 ¹⁴C-yr BP. Relatively high lake levels were confirmed by ¹⁴C-dated aquatic gastropods from subaerial soil profiles ~ 1.0–7.5 m above present lake stage. High lake levels were a consequence of lower E/P and/or greater surface runoff and groundwater inflow caused by human-induced deforestation.     

A ∼6100 14C yr record of El Niño activity from the Galápagos Islands

Lithostratigrahic and mineralogic analyses of sediments from hypersaline Bainbridge Crater Lake, Galápagos Islands, provide evidence of past El Niño frequency and intensity. Laminated sediments indicate that at least 435 moderate to very strong El Niño events have occurred since 6100 ¹⁴C yr BP (7130 cal yr BP), and that frequency and intensity of events increased at about 3000 ¹⁴C yr BP (3100 cal yr BP). El Niño activity was present between 6100 and 4000 ¹⁴C yr BP (4600 cal yr BP) but infrequent. The Bainbridge record indicates that there has been considerable millennial-scale variability in El Niño since the mid-Holocene.     

Map-Based Exploratory Evaluation of Non-Medical Determinants of Population Health

Multi‐criteria evaluation (MCE) and decision‐making are increasingly combined with interactive tools to assist users with visual thinking and exploring decision strategies. The interactive control of criterion combination rules and the simultaneous observation of geographic space and criterion space provide a means of investigating the sensitivity of the decision outcome to the decision‐maker's preferences. The Analytic Hierarchy Process (AHP) is an MCE method that has been successfully implemented in management processes including those addressed by Geographic Information Systems. In this paper, we present a map‐based, interactive AHP implementation, which provides a link between a well‐understood decision support method and exploratory geographic visualization. Using a case study with public health data for the Province of Ontario, Canada, we demonstrate that exploratory map use increases the effectiveness of the AHP‐based evaluation of population health.