Denitrification has been measured during the last few years using two different methods in particular: isotope pairing measured on a triple-collector isotopic ratio mass spectrometer and N
2:Ar ratios measured on a membrane inlet mass spectrometer (MIMS). This study compares these two techniques in short-term batch experiments. Rates obtained using the original N
2∶Ar method were up to 3 to 4 times higher than rates obtained using the isotope pairing technique due to O
2 reacting with the N
2 during MIMS analysis. Oxygen combines with N
2 within the mass spectrometer ion source forming NO
+ which reduces the N
2 concentration. The decrease in N
2 is least at lower O
2 concentrations and since oxygen is typically consumed during incubations of sediment cores, the result is often a pseudo-increase in N
2 concentration being interpreted as denitrification activity. The magnitude of this ocygen effect may be instrument specific. The reaction of O
2 with N
2 and the subsequent decrease in N
2 was only partly correctly using an O
2 correction curve for the relationship between N
2 and O
2 concentrations. The O
2 corrected N
2∶Ar denitrification rates were lower, but still did not match the isotope pairing rates and the variability between replicates was much higher. Using a copper reduction column heated to 600°C to remove all of the O
2 from the sample before MIMS analysis resulted in comparable rates (slightly lower), and comparable variability between replicates, to the isotope pairing technique. The N
2:Ar technique determines the net N
2 production as the difference between N
2 production by denitrification and N
2 consumption by N-fixation, while N-fixation has little effect on the isotope pairing technique which determines a rate very close to the gross N
2 production. When the two different techniques were applied on the same sediment, the small difference in rates obtained by the two methods seemed to reflect N-fixation as also supported from measurements of ethylene production in acetylene enriched sediment cores. The N
2:Ar and isotope pairing techniques may be combined to provide simultaneous measurements of denitrification and N-fixation. Both techniques have several assumptions that must be met to achieve accurate rates; a number of tests are outlined that can be applied to demonstrate that these assumptions are being meet.
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